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抗人CD5 抗体,克隆UCHT2

小鼠单克隆IgG1抗体,抗人、恒河猴、食蟹猴CD5

产品号 #(选择产品)

产品号 #60082_C

小鼠单克隆IgG1抗体,抗人、恒河猴、食蟹猴CD5

总览

UCHT2抗体识别CD5,CD5是一种~67 kDa的单链I型糖蛋白,是清道夫受体超家族的成员,在胸腺细胞、T细胞、B细胞亚群、外周血树突状细胞和一些白血病和淋巴瘤细胞,包括慢性B淋巴细胞白血病(B- CLL)细胞上组成性表达。CD5在胸腺细胞上低水平表达,而在成熟T细胞上高密度表达。可能的配体包括CD5L和CD72。CD5通过其与T细胞受体/CD3复合物和B细胞受体的物理和功能关联,调节T和B细胞受体信号传导、胸腺细胞成熟和T细胞/B细胞相互作用。对CD5敲除小鼠的研究表明,CD5负调控胸腺细胞和成熟T细胞中抗原受体介导的信号传导。UCHT2抗体结合位于CD5细胞外结构域的表位2(epitope 2)。

亚型
一抗
 
靶抗原
CD5
 
别名
Leu1,Leu-1,Ly-1,淋巴细胞抗原T1,T1,Tp67
 
活性物种
Capuchin Monkey,Chimpanzee,Common Marmoset,Cynomolgus,人,Owl Monkey,Rhesus
 
偶联
APC,Biotin 或 生物素,FITC,PE,PerCP-Cyanine5.5,未偶联的
 
宿主物种
小鼠
 
细胞类型
T 细胞
 
种属
人,非人灵长类
 
应用
流式细胞术,免疫细胞化学,免疫组化,Western印迹
 
研究领域
免疫,干细胞生物学
 
克隆
UCHT2
 
基因编号
921
 
同种型
IgG1,kappa
 

实验数据

Data for APC-Conjugated

Figure 1. Data for APC-Conjugated

(A) Flow cytometry analysis of human buffy coat nucleated cells labeled with Anti-Human CD5 Antibody, Clone UCHT2, APC and Anti-Human CD20 Antibody, Clone 2H7, Alexa Fluor® 488 (Catalog #60008AD). (B) Flow cytometry analysis of human buffy coat nucleated cells labeled with a mouse IgG1, kappa APC isotype control antibody and Anti-Human CD20 Antibody, Clone 2H7, Alexa Fluor® 488. (C) Flow cytometry analysis of human buffy coat nucleated cells processed with the EasySep™ HLA Whole Blood CD3 Positive Selection Kit and labeled with Anti-Human CD5 Antibody, Clone UCHT2, APC. Histograms show labeling of buffy coat nucleated cells (Start) and isolated cells (Isolated). Labeling of start cells with a mouse IgG1, kappa APC isotype control antibody is shown (solid line histogram).

Data for Biotin-Conjugated

Figure 2. Data for Biotin-Conjugated

(A) Flow cytometry analysis of human buffy coat nucleated cells labeled with Anti-Human CD5 Antibody, Clone UCHT2, Biotin followed by streptavidin (SAV) APC and Anti-Human CD20 Antibody, Clone 2H7, PE (Catalog #60008PE).
(B) Flow cytometry analysis of human buffy coat nucleated cells labeled with a mouse IgG1, kappa biotin isotype control antibody followed by streptavidin (SAV) APC and Anti-Human CD20 Antibody, Clone 2H7, PE.
(C) Flow cytometry analysis of human buffy coat nucleated cells processed with the EasySep™ HLA CD3 Positive Selection Kit and labeled with Anti-Human CD5 Antibody, Clone UCHT2, Biotin followed by streptavidin (SAV) APC. Histograms show labeling of buffy coat nucleated cells (Start) and isolated cells (Isolated). Labeling of start cells with a mouse IgG1, kappa biotin isotype control antibody followed by SAV APC is shown (open histogram).

Data for FITC-Conjugated

Figure 3. Data for FITC-Conjugated

(A) Flow cytometry analysis of human buffy coat nucleated cells labeled with Anti-Human CD5 Antibody, Clone UCHT2, FITC and Anti-Human CD20 Antibody, Clone 2H7, APC (Catalog #60008AZ). (B) Flow cytometry analysis of human buffy coat nucleated cells labeled with a mouse IgG1, kappa FITC isotype control antibody and Anti-Human CD20 Antibody, Clone 2H7, APC. (C) Flow cytometry analysis of human buffy coat nucleated cells processed with the EasySep™ HLA CD3 Positive Selection Kit and labeled with Anti-Human CD5 Antibody, Clone UCHT2, FITC. Histograms show labeling of buffy coat nucleated cells (Start) and isolated cells (Isolated). Labeling of start cells with a mouse IgG1, kappa FITC isotype control antibody is shown (solid line histogram).

Data for PE-Conjugated

Figure 4. Data for PE-Conjugated

(A) Flow cytometry analysis of human buffy coat nucleated cells labeled with Anti-Human CD5 Antibody, Clone UCHT2, PE and Anti-Human CD20 Antibody, Clone 2H7, APC (Catalog #60008AZ). (B) Flow cytometry analysis of human buffy coat nucleated cells labeled with a mouse IgG1, kappa PE isotype control antibody and Anti-Human CD20 Antibody, Clone 2H7, APC. (C) Flow cytometry analysis of human buffy coat nucleated cells processed with the EasySep™ HLA CD3 Positive Selection Kit and labeled with Anti-Human CD5 Antibody, Clone UCHT2, PE. Histograms show labeling of buffy coat nucleated cells (Start) and isolated cells (Isolated). Labeling of start cells with a mouse IgG1, kappa PE isotype control antibody is shown (solid line histogram).

Data for Unconjugated

Figure 5. Data for Unconjugated

Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Human CD5 Antibody, Clone UCHT2, followed by Goat Anti-Mouse IgG (H+L) Antibody, Polyclonal, FITC (Catalog #60138FI) (filled histogram), or a mouse IgG1, kappa isotype control antibody, followed by Goat Anti-Mouse IgG (H+L) Antibody, Polyclonal, FITC (solid line histogram).

Data for PerCP-Cy55-Conjugated

Figure 6. Data for PerCP-Cy55-Conjugated

Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Human CD5 Antibody, Clone UCHT2, PerCP-Cy5.5 (filled histogram) or a mouse IgG1, kappa isotype control antibody, PerCP-Cy5.5 (solid line histogram).

产品说明书及文档

请在《产品说明书》中查找相关支持信息和使用说明,或浏览下方更多实验方案。

Document Type
Product Name
Catalog #
Lot #
Language
Catalog #
60082AZ, 60082AZ.1
Lot #
All
Language
English
Catalog #
60082BT
Lot #
All
Language
English
Catalog #
60082FI, 60082FI.1
Lot #
All
Language
English
Catalog #
60082PE, 60082PE.1
Lot #
All
Language
English
Catalog #
60082PS
Lot #
All
Language
English
Catalog #
60082
Lot #
All
Language
English
Document Type
Safety Data Sheet
Catalog #
60082AZ, 60082AZ.1
Lot #
All
Language
English
Document Type
Safety Data Sheet
Catalog #
60082BT
Lot #
All
Language
English
Document Type
Safety Data Sheet
Catalog #
60082FI, 60082FI.1
Lot #
All
Language
English
Document Type
Safety Data Sheet
Catalog #
60082PE, 60082PE.1
Lot #
All
Language
English
Document Type
Safety Data Sheet
Catalog #
60082PS
Lot #
All
Language
English
Document Type
Safety Data Sheet
Catalog #
60082
Lot #
All
Language
English

应用领域

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相关材料与文献

技术资料 (2)

更多信息

更多信息
种属 Human, Non-Human Primate
克隆 UCHT2
Gene Id 921
Alternative Names Leu1, Leu-1, Ly-1, Lymphocyte antigen T1, T1, Tp67
同种型/isotype IgG1, kappa
质量保证:

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