产品号 #60082_C
小鼠单克隆IgG1抗体,抗人、恒河猴、食蟹猴CD5
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UCHT2抗体识别CD5,CD5是一种~67 kDa的单链I型糖蛋白,是清道夫受体超家族的成员,在胸腺细胞、T细胞、B细胞亚群、外周血树突状细胞和一些白血病和淋巴瘤细胞,包括慢性B淋巴细胞白血病(B- CLL)细胞上组成性表达。CD5在胸腺细胞上低水平表达,而在成熟T细胞上高密度表达。可能的配体包括CD5L和CD72。CD5通过其与T细胞受体/CD3复合物和B细胞受体的物理和功能关联,调节T和B细胞受体信号传导、胸腺细胞成熟和T细胞/B细胞相互作用。对CD5敲除小鼠的研究表明,CD5负调控胸腺细胞和成熟T细胞中抗原受体介导的信号传导。UCHT2抗体结合位于CD5细胞外结构域的表位2(epitope 2)。
亚型
一抗
靶抗原
CD5
别名
Leu1,Leu-1,Ly-1,淋巴细胞抗原T1,T1,Tp67
活性物种
Capuchin Monkey,Chimpanzee,Common Marmoset,Cynomolgus,人,Owl Monkey,Rhesus
偶联
APC,Biotin 或 生物素,FITC,PE,PerCP-Cyanine5.5,未偶联的
宿主物种
小鼠
细胞类型
T 细胞
种属
人,非人灵长类
应用
流式细胞术,免疫细胞化学,免疫组化,Western印迹
研究领域
免疫,干细胞生物学
克隆
UCHT2
基因编号
921
同种型
IgG1,kappa
Figure 1. Data for APC-Conjugated
(A) Flow cytometry analysis of human buffy coat nucleated cells labeled with Anti-Human CD5 Antibody, Clone UCHT2, APC and Anti-Human CD20 Antibody, Clone 2H7, Alexa Fluor® 488 (Catalog #60008AD). (B) Flow cytometry analysis of human buffy coat nucleated cells labeled with a mouse IgG1, kappa APC isotype control antibody and Anti-Human CD20 Antibody, Clone 2H7, Alexa Fluor® 488. (C) Flow cytometry analysis of human buffy coat nucleated cells processed with the EasySep™ HLA Whole Blood CD3 Positive Selection Kit and labeled with Anti-Human CD5 Antibody, Clone UCHT2, APC. Histograms show labeling of buffy coat nucleated cells (Start) and isolated cells (Isolated). Labeling of start cells with a mouse IgG1, kappa APC isotype control antibody is shown (solid line histogram).
Figure 2. Data for Biotin-Conjugated
(A) Flow cytometry analysis of human buffy coat nucleated cells labeled with Anti-Human CD5 Antibody, Clone UCHT2, Biotin followed by streptavidin (SAV) APC and Anti-Human CD20 Antibody, Clone 2H7, PE (Catalog #60008PE).
(B) Flow cytometry analysis of human buffy coat nucleated cells labeled with a mouse IgG1, kappa biotin isotype control antibody followed by streptavidin (SAV) APC and Anti-Human CD20 Antibody, Clone 2H7, PE.
(C) Flow cytometry analysis of human buffy coat nucleated cells processed with the EasySep™ HLA CD3 Positive Selection Kit and labeled with Anti-Human CD5 Antibody, Clone UCHT2, Biotin followed by streptavidin (SAV) APC. Histograms show labeling of buffy coat nucleated cells (Start) and isolated cells (Isolated). Labeling of start cells with a mouse IgG1, kappa biotin isotype control antibody followed by SAV APC is shown (open histogram).
Figure 3. Data for FITC-Conjugated
(A) Flow cytometry analysis of human buffy coat nucleated cells labeled with Anti-Human CD5 Antibody, Clone UCHT2, FITC and Anti-Human CD20 Antibody, Clone 2H7, APC (Catalog #60008AZ). (B) Flow cytometry analysis of human buffy coat nucleated cells labeled with a mouse IgG1, kappa FITC isotype control antibody and Anti-Human CD20 Antibody, Clone 2H7, APC. (C) Flow cytometry analysis of human buffy coat nucleated cells processed with the EasySep™ HLA CD3 Positive Selection Kit and labeled with Anti-Human CD5 Antibody, Clone UCHT2, FITC. Histograms show labeling of buffy coat nucleated cells (Start) and isolated cells (Isolated). Labeling of start cells with a mouse IgG1, kappa FITC isotype control antibody is shown (solid line histogram).
Figure 4. Data for PE-Conjugated
(A) Flow cytometry analysis of human buffy coat nucleated cells labeled with Anti-Human CD5 Antibody, Clone UCHT2, PE and Anti-Human CD20 Antibody, Clone 2H7, APC (Catalog #60008AZ). (B) Flow cytometry analysis of human buffy coat nucleated cells labeled with a mouse IgG1, kappa PE isotype control antibody and Anti-Human CD20 Antibody, Clone 2H7, APC. (C) Flow cytometry analysis of human buffy coat nucleated cells processed with the EasySep™ HLA CD3 Positive Selection Kit and labeled with Anti-Human CD5 Antibody, Clone UCHT2, PE. Histograms show labeling of buffy coat nucleated cells (Start) and isolated cells (Isolated). Labeling of start cells with a mouse IgG1, kappa PE isotype control antibody is shown (solid line histogram).
Figure 5. Data for Unconjugated
Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Human CD5 Antibody, Clone UCHT2, followed by Goat Anti-Mouse IgG (H+L) Antibody, Polyclonal, FITC (Catalog #60138FI) (filled histogram), or a mouse IgG1, kappa isotype control antibody, followed by Goat Anti-Mouse IgG (H+L) Antibody, Polyclonal, FITC (solid line histogram).
Figure 6. Data for PerCP-Cy55-Conjugated
Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Human CD5 Antibody, Clone UCHT2, PerCP-Cy5.5 (filled histogram) or a mouse IgG1, kappa isotype control antibody, PerCP-Cy5.5 (solid line histogram).
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种属 | Human, Non-Human Primate |
---|---|
克隆 | UCHT2 |
Gene Id | 921 |
Alternative Names | Leu1, Leu-1, Ly-1, Lymphocyte antigen T1, T1, Tp67 |
同种型/isotype | IgG1, kappa |
小鼠单克隆IgG1, kappa同型对照抗体
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