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抗人OCT4（OCT3）抗体，克隆3A2A20

小鼠单克隆IgG2b抗体，抗人OCT4（OCT3）
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产品号 #60093_C

小鼠单克隆IgG2b抗体，抗人OCT4（OCT3）

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总览
实验数据
产品说明书及文档
应用领域
相关材料与文献
相关产品

总览

该单克隆抗体可识别人八聚体结合转录因子4（OCT4），又称 OCT3 或 OCT3/4，是一种分子量约为 40 kDa 的同源域转录因子，隶属于 POU 转录因子家族。在未分化的人胚胎干细胞（ES）、诱导多能干细胞（iPS）、胚胎癌细胞（EC）和胚胎生殖细胞（EG）中均有表达。OCT4 能结合八聚体基序 5'-ATTTGCAT-3'，并通过与其他转录因子（如 SOX2）相互作用，调控多个基因的表达，包括 FBX15、FGF-4、REX1、SOX2 和骨桥蛋白（osteopontin），在维持细胞多能性方面发挥关键作用。OCT4 的表达水平在细胞分化过程中会下调，因此被广泛用作干细胞多能性的标志物，同时也是某些人类恶性生殖细胞肿瘤的诊断标志物之一。OCT4 与其他转录因子共同表达，已被用于将体细胞重编程为 iPS 细胞。目前已发现 OCT4 存在多种剪接亚型，其中至少有两种在人类中具有功能活性。

亚型
一抗
 
靶抗原
OCT4 (OCT3)
 
别名
OCT-3；OCT-4；OCT3；八聚体结合转录因子 4；POU 结构域类 5 转录因子 1；POU5F1
 
活性物种
人
 
偶联
Alexa Fluor 488，PE，未偶联的
 
宿主物种
小鼠
 
细胞类型
多能干细胞
 
种属
人
 
应用
流式细胞术，免疫细胞化学，免疫荧光，Western印迹
 
研究领域
干细胞生物学
 
克隆
3A2A20
 
基因编号
5460
 
同种型
IgG2b，kappa
 

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实验数据

Data for Alexa Fluor® 488-Conjugated

Figure 1. Data for Alexa Fluor® 488-Conjugated

(A) Flow cytometry analysis of ES cells cultured with mTeSR™1 on Corning® Matrigel®. The ES cells (filled histogram) or HT1080 fibrosarcoma cells (negative control; dashed line histogram) were fixed and labeled with Anti-Human OCT4 (OCT3) Antibody, Clone 3A2A20, Alexa Fluor® 488. Labeling of the ES cells with Mouse IgG2b, kappa Isotype Control Antibody, Clone MPC-11, Alexa Fluor® 488 (Catalog #60072AD) is shown (solid line histogram). (B) Human ES cells were cultured with TeSR™-E8™ on glass coverslips coated with Vitronectin XF™ (Catalog #07180), then fixed and labeled with Anti-Human OCT4 (OCT3) Antibody, Clone 3A2A20, Alexa Fluor® 488. Inset shows labeling of human ES cells with Mouse IgG2b, kappa Isotype Control Antibody, Clone MPC-11, Alexa Fluor® 488. (C) DAPI counterstaining of the cells shown in figure (B); nuclear localization of the OCT4 (OCT3) marker is evident.

Data for PE-Conjugated

Figure 2. Data for PE-Conjugated

(A) Flow cytometry analysis of human ES cells cultured with mTeSR™1 on Corning® Matrigel®. The ES cells (filled histogram) or HT1080 fibrosarcoma cells (negative control; dashed line histogram) were fixed and labeled with Anti-Human OCT4 (OCT3) Antibody, Clone 3A2A20, PE. Labeling of the ES cells with Mouse IgG2b, kappa Isotype Control Antibody, Clone MPC-11, PE (Catalog #60072PE) is shown (solid line histogram). (B) Human ES cells were cultured with TeSR™-E8™ on glass coverslips coated with Vitronectin XF™ (Catalog #07180), then fixed and labeled with Anti-Human OCT4 (OCT3) Antibody, Clone 3A2A20, PE. Inset shows labeling of human ES cells with Mouse IgG2b, kappa Isotype Control Antibody, Clone MPC-11, PE. (C) DAPI counterstaining of the cells shown in figure (B); nuclear localization of the OCT4 (OCT3) marker is evident.

Data for Unconjugated

Figure 3. Data for Unconjugated

(A) Human induced pluripotent stem (iPS) cells were cultured with TeSR™-E8™ on glass coverslips coated with Vitronectin XF™ (Catalog #07180), then fixed and stained with Anti-Human OCT4 (OCT3) Antibody, Clone 3A2A20, followed by goat anti-mouse IgG, FITC. Inset shows cells labeled with a mouse IgG2b, kappa isotype control antibody followed by goat anti-mouse IgG, FITC.
(B) Flow cytometry analysis of human embryonic stem (ES) cells cultured with mTeSR™1 on Corning® Matrigel®. The ES cells (filled histogram) or HT1080 fibrosarcoma cells (negative control, dashed line histogram) were fixed and labeled with Anti-Human OCT4 (OCT3) Antibody, Clone 3A2A20, followed by goat anti-mouse IgG, FITC. Labeling of the ES cells with a mouse IgG2b, kappa isotype control antibody followed by goat anti-mouse IgG, FITC is shown (solid line histogram). (C) Flow cytometry analysis of human iPS cells cultured with TeSR™-E8™ on Vitronectin XF™. The cells were fixed and labeled with Anti-Human OCT4 (OCT3) Antibody, Clone 3A2A20, followed by goat anti-mouse IgG, FITC (filled histogram) or a mouse IgG2b, kappa isotype control antibody followed by goat anti-mouse IgG, FITC (solid line histogram). (D) Western blot analysis of denatured/reduced cell lysates with Anti-Human OCT4 (OCT3) Antibody, Clone 3A2A20. Lane 1, human ES cells cultured with mTeSR™1 on Corning® Matrigel®, lane 2 (negative control), mouse E13.5 neural progenitor cells cultured with NeuroCult™ Proliferation Kit (Mouse, Catalog #05702).

产品说明书及文档

请在《产品说明书》中查找相关支持信息和使用说明，或浏览下方更多实验方案。

Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet
Product Name
Anti-Human OCT4 (OCT3) Antibody, Clone 3A2A20
Catalog #
60093.1, 60093
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Human OCT4 (OCT3) Antibody, Clone 3A2A20, PE
Catalog #
60093PE.1, 60093PE
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Human OCT4 (OCT3) Antibody, Clone 3A2A20, Alexa Fluor® 488
Catalog #
60093AD.1, 60093AD
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Human OCT4 (OCT3) Antibody, Clone 3A2A20
Catalog #
60093.1, 60093
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Human OCT4 (OCT3) Antibody, Clone 3A2A20, PE
Catalog #
60093PE.1, 60093PE
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Human OCT4 (OCT3) Antibody, Clone 3A2A20, Alexa Fluor® 488
Catalog #
60093AD.1, 60093AD
Lot #
All
Language
English

应用领域

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相关材料与文献

技术资料 (3)

Reliable Antibodies for Your Research
Brochure
Reliable Antibodies for Your Research
hPSC Differentiation: Tools for Pluripotent Stem Cell-Derived Research
Brochure
hPSC Differentiation: Tools for Pluripotent Stem Cell-Derived Research
Products for Human Pluripotent Stem Cells
Brochure
Products for Human Pluripotent Stem Cells

文献 (2)

Trend of telomerase activity change during human iPSC self-renewal and differentiation revealed by a quartz crystal microbalance based assay. Zhou Y et al. Scientific reports 2014 NOV

Abstract

Telomerase plays an important role in governing the life span of cells for its capacity to extend telomeres. As high activity of telomerase has been found in stem cells and cancer cells specifically, various methods have been developed for the evaluation of telomerase activity. To overcome the time-consuming procedures and complicated manipulations of existing methods, we developed a novel method named Telomeric Repeat Elongation Assay based on Quartz crystal microbalance (TREAQ) to monitor telomerase activity during the self-renewal and differentiation of human induced pluripotent stem cells (hiPSCs). TREAQ results indicated hiPSCs possess invariable telomerase activity for 11 passages on Matrigel and a steady decline of telomerase activity when differentiated for different periods, which is confirmed with existing golden standard method. The pluripotency of hiPSCs during differentiation could be estimated through monitoring telomerase activity and compared with the expression levels of markers of pluripotency gene via quantitative real time PCR. Regular assessment for factors associated with pluripotency or stemness was expensive and requires excessive sample consuming, thus TREAQ could be a promising alternative technology for routine monitoring of telomerase activity and estimate the pluripotency of stem cells.
View publication
Evidences for the involvement of cell surface glycans in stem cell pluripotency and differentiation Alisson-Silva F et al. Glycobiology 2014 MAY

Abstract

Induced pluripotent stem (iPS) cells are somatic cells that have been reprogrammed to a pluripotent state via the introduction of defined transcription factors. Although iPS is a potentially valuable resource for regenerative medicine and drug development, several issues regarding their pluripotency, differentiation propensity and potential for tumorigenesis remain to be elucidated. Analysis of cell surface glycans has arisen as an interesting tool for the characterization of iPS. An appropriate characterization of glycan surface molecules of human embryonic stem (hES) cells and iPS cells might generate crucial data to highlight their role in the acquisition and maintenance of pluripotency. In this study, we characterized the surface glycans of iPS generated from menstrual blood-derived mesenchymal cells (iPS-MBMC). We demonstrated that, upon spontaneous differentiation, iPS-MBMC present high amounts of terminal $\$-galactopyranoside residues, pointing to an important role of terminal-linked sialic acids in pluripotency maintenance. The removal of sialic acids by neuraminidase induces iPS-MBMC and hES cells differentiation, prompting an ectoderm commitment. Exposed $\$-galactopyranose residues might be recognized by carbohydrate-binding molecules found on the cell surface, which could modulate intercellular or intracellular interactions. Together, our results point for the first time to the involvement of the presence of terminal sialic acid in the maintenance of embryonic stem cell pluripotency and, therefore, the modulation of sialic acid biosynthesis emerges as a mechanism that may govern stem cell differentiation.
View publication
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更多信息

更多信息
种属 Human
克隆 3A2A20
Gene Id 5460
Alternative Names OCT-3, OCT-4, OCT3, octamer-binding transcription factor 4, POU domain class 5 transcription factor 1, POU5F1
同种型/isotype IgG2b, kappa
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