抗小鼠CD117抗体，克隆2B8

大鼠单克隆IgG2b抗体，抗小鼠CD117（c-Kit）

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产品号 #（选择产品）

产品号 #60025_C

大鼠单克隆IgG2b抗体，抗小鼠CD117（c-Kit）

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总览

实验数据

产品说明书及文档

应用领域

总览

2B8抗体识别CD117 （c-Kit），CD117 （c-Kit）是一种~145 kDa的c-Kit配体（干细胞因子/钢铁因子）的1型跨膜受体，广泛表达于骨髓造血干细胞，包括多能祖细胞、红系祖细胞、B和T淋巴细胞前体，以及肥大细胞。CD117属于酪氨酸激酶受体家族，具有5个免疫球蛋白样C2型结构域和1个细胞质蛋白激酶结构域。c-Kit配体与CD117结合可诱导二聚化和自磷酸化，从而激活对造血干细胞增殖和分化至关重要的几种细胞内信号通路。CD117信号通过磷酸酶和受体的快速内吞和降解来调控。2B8抗体不会阻断c-Kit配体的结合。CD117的突变与各种类型的肿瘤和骓斑（一种常染色体显性色素沉着异常）有关。

该抗体克隆已被验证可用于评估EasySep™试剂盒（包括EasySep™小鼠造血祖细胞分选试剂盒（产品号 #19856））分选的细胞纯度。

亚型
一抗

靶抗原
CD117 (c-Kit)

别名
c-KIT，cKIT，干细胞因子受体（SCFR）

活性物种
小鼠

偶联
Alexa Fluor 488，APC，Biotin 或生物素，FITC，PE，未偶联的

宿主物种
大鼠

细胞类型
造血干/祖细胞

种属
小鼠

应用
细胞分选，流式细胞术，免疫细胞化学，免疫荧光，免疫组化，免疫沉淀

研究领域
干细胞生物学

克隆
2B8

基因编号
16590

同种型
IgG2b，kappa

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实验数据

Figure 1. Data for Alexa Fluor® 488-Conjugated

(A) Flow cytometry analysis of C57BL/6 mouse bone marrow cells labeled with Anti-Mouse CD117 Antibody, Clone 2B8, Alexa Fluor® 488 and anti-mouse CD45R APC. (B) Flow cytometry analysis of C57BL/6 mouse bone marrow cells pre-labeled with Anti-Mouse CD117 Antibody, Clone 2B8, Alexa Fluor® 488 and processed with the EasySep™ Mouse CD117 Positive Selection Kit (Catalog #18757). Histograms show labeling of bone marrow (Start) and isolated cells (Isolated); partial blocking may be observed. Labeling with a rat IgG2b, kappa Alexa Fluor® 488 isotype control antibody is shown (solid line histogram).

Figure 2. Data for PE-Conjugated

(A) Flow cytometry analysis of C57BL/6 mouse bone marrow cells labeled with Anti-Mouse CD117 Antibody, Clone 2B8, PE and anti-mouse CD45R APC. (B) Flow cytometry analysis of C57BL/6 mouse bone marrow cells processed with the EasySep™ Mouse PE Positive Selection Kit (Catalog #18554), and labeled with Anti-Mouse CD117 Antibody, Clone 2B8, PE. Histograms show labeling of bone marrow (Start) and isolated cells (Isolated). Labeling with a rat IgG2b, kappa PE isotype control antibody is shown (solid line histogram).

Figure 3. Data for Unconjugated

(A) Flow cytometry analysis of C57BL/6 mouse bone marrow cells labeled with Anti-Mouse CD117 (c-Kit) Antibody, Clone 2B8, followed by a mouse anti-rat IgG2b antibody, FITC and Anti-Mouse CD45R Antibody, Clone RA3-6B2, PE (Catalog #60019PE). (B) Flow cytometry analysis of C57BL/6 mouse bone marrow cells labeled with Rat IgG2b, kappa Isotype Control Antibody, Clone RTK4530 (Catalog #60077), followed by a mouse anti-rat IgG2b antibody, FITC and Anti-Mouse CD45R Antibody, Clone RA3-6B2, PE.

Figure 4. Data for APC-Conjugated

(A) Flow cytometry analysis of C57BL/6 mouse bone marrow cells labeled with Anti-Mouse CD117 Antibody, Clone 2B8, APC and Anti-Mouse CD45R Antibody, Clone RA3-6B2, FITC (Catalog #60019FI). (B) Flow cytometry analysis of C57BL/6 mouse bone marrow cells labeled with Rat IgG2b, kappa Isotype Control Antibody, Clone RTK4530, APC (Catalog #60077AZ) and Anti-Mouse CD45R Antibody, Clone RA3-6B2, FITC.

Figure 5. Data for Biotin-Conjugated

(A) Flow cytometry analysis of C57BL/6 mouse bone marrow cells labeled with Anti-Mouse CD117 Antibody, Clone 2B8, Biotin, followed by streptavidin (SAV) APC and Anti-Mouse CD45R Antibody, Clone RA3-6B2, FITC (Catalog #60019FI). (B) Flow cytometry analysis of C57BL/6 mouse bone marrow cells labeled with Rat IgG2b, kappa Isotype Control Antibody, Clone RTK4530, Biotin (Catalog #60077BT), followed by SAV APC and Anti-Mouse CD45R Antibody, Clone RA3-6B2, FITC.

Figure 6. Data for FITC-Conjugated

(A) Flow cytometry analysis of C57BL/6 mouse bone marrow cells labeled with Anti-Mouse CD117 Antibody, Clone 2B8, FITC and Anti-Mouse CD45R Antibody, Clone RA3-6B2, APC (Catalog #60019AZ). (B) Flow cytometry analysis of C57BL/6 mouse bone marrow cells labeled with Rat IgG2b, kappa Isotype Control Antibody, Clone RTK4530, FITC (Catalog #60077FI) and Anti-Mouse CD45R Antibody, Clone RA3-6B2, APC.