抗小鼠CD117抗体，克隆ACK2

大鼠抗小鼠CD117（c-Kit）单克隆IgG2b抗体

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产品号 #（选择产品）

产品号 #60034_C

大鼠抗小鼠CD117（c-Kit）单克隆IgG2b抗体

总览

实验数据

产品说明书及文档

应用领域

总览

ACK2 单克隆抗体可识别 CD117（也称为 c-Kit），这是一种分子量约为 145 kDa 的 I 型跨膜受体，是 c-Kit 配体（即干细胞因子 / steel 因子）的受体。CD117 广泛表达于骨髓中的造血干细胞，包括多能干细胞、红系祖细胞以及 B 和 T 淋巴细胞前体细胞，同时也表达于肥大细胞表面。

CD117 属于酪氨酸激酶受体家族，结构上具有五个免疫球蛋白样的 C2 型结构域和一个胞质蛋白激酶结构域。当 c-Kit 配体与 CD117 结合后，可诱导其二聚化和自磷酸化，从而激活多个对造血干细胞的增殖和分化至关重要的细胞内信号通路。

CD117 的信号传导受到磷酸酶调控，以及受体的快速内吞和降解过程的调节。CD117 基因的突变与多种类型的肿瘤及“斑驳病”（piebald trait，一种常染色体显性遗传的色素异常）有关。

据报道，ACK2 抗体的结合可阻断 CD117 的功能。

分类
一抗

靶抗原
CD117 (c-Kit)

别名
c-KIT，cKIT，干细胞因子受体（SCFR）

反应种属
小鼠

偶联
Alexa Fluor 488，APC，FITC，PE，未偶联

宿主物种
大鼠

细胞类型
造血干/祖细胞

种属
小鼠

应用
流式细胞术，功能学筛选，免疫细胞化学，免疫荧光，免疫组化，免疫沉淀，Western印迹

研究领域
干细胞生物学

克隆
ACK2

Gene ID
16590

Isotype
IgG2b, κ轻链

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实验数据

Figure 1. Data for PE-Conjugated

Flow cytometry analysis of C57BL/6 mouse bone marrow cells labeled with Anti-Mouse CD117 Antibody, Clone ACK2, PE (filled histogram) or a rat IgG2b, kappa PE isotype control antibody (solid line histogram).

Figure 2. Data for Unconjugated

(A) Flow cytometry analysis of C57BL/6 mouse bone marrow cells labeled with Anti-Mouse CD117 Antibody, Clone ACK2, followed by a mouse anti-rat IgG2b antibody, FITC and Anti-Mouse CD45R Antibody, Clone RA3-6B2, PE (Catalog #60019PE). (B) Flow cytometry analysis of C57BL/6 mouse bone marrow cells labeled with Rat IgG2b, kappa Isotype Control Antibody, Clone RTK4530 (Catalog #60077), followed by a mouse anti-rat IgG2b antibody, FITC and Anti-Mouse CD45R Antibody, Clone RA3-6B2, PE.

Figure 3. Data for AlexaFluor® 488-Conjugated

(A) Flow cytometry analysis of C57BL/6 mouse bone marrow cells labeled with Anti-Mouse CD117 Antibody, Clone ACK2, Alexa Fluor® 488 and Anti-Mouse CD45R Antibody, Clone RA3-6B2, APC (Catalog #60019AZ). (B) Flow cytometry analysis of C57BL/6 mouse bone marrow cells labeled with Rat IgG2b, kappa Isotype Control Antibody, Clone RTK4530, Alexa Fluor® 488 (Catalog #60077AD) and Anti-Mouse CD45R Antibody, Clone RA3-6B2, APC.

Figure 4. Data for APC-Conjugated

(A) Flow cytometry analysis of C57BL/6 mouse bone marrow cells labeled with Anti-Mouse CD117 Antibody, Clone ACK2, APC and Anti-Mouse CD45R Antibody, Clone RA3-6B2, FITC (Catalog #60019FI). (B) Flow cytometry analysis of C57BL/6 mouse bone marrow cells labeled with Rat IgG2b, kappa Isotype Control Antibody, Clone RTK4530, APC (Catalog #60077AZ) and Anti-Mouse CD45R Antibody, Clone RA3-6B2, FITC.

Figure 5. Data for FITC-Conjugated

(A) Flow cytometry analysis of C57BL/6 mouse bone marrow cells labeled with Anti-Mouse CD117 Antibody, Clone ACK2, FITC and Anti-Mouse CD45R Antibody, Clone RA3-6B2, APC (Catalog #60019AZ). (B) Flow cytometry analysis of C57BL/6 mouse bone marrow cells labeled with Rat IgG2b, kappa Isotype Control Antibody, Clone RTK4530, FITC (Catalog #60077FI) and Anti-Mouse CD45R Antibody, Clone RA3-6B2, APC.