Anti-Mouse CD19 Antibody, Clone 1D3

总览

1D3抗体识别小鼠CD19，CD19是一种~95 kDa的1型跨膜糖蛋白，在从早期pre-B细胞到浆细胞的所有发育阶段的B细胞表面表达，在终末分化的浆细胞中表达下调但仍然存在。CD19也存在于滤泡树突状细胞中。CD19通过与CD21和CD81相结合，作为B细胞受体的共受体，参与B细胞的活化和分化。CD19的激活伴随着细胞质结构域的磷酸化，从而促进与激酶的结合并诱导细胞内信号级联反应。CD19的突变可导致严重免疫缺陷综合征。交叉竞争分析发现抗体克隆1D3与克隆6D5识别相同表位。

亚型
一抗

靶抗原
CD19

别名
B4

活性物种
小鼠

偶联
APC，Biotin 或生物素，FITC，PE，PerCP-Cyanine5.5，未偶联的

宿主物种
大鼠

细胞类型
B 细胞

种属
小鼠

应用
流式细胞术，功能学筛选，免疫细胞化学，免疫荧光，免疫组化，免疫沉淀

研究领域
免疫

克隆
1D3

基因编号
12478

同种型
IgG2a，kappa

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实验数据

Figure 1. Data for Unconjugated

Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD19 Antibody, Clone 1D3, followed by a mouse anti-rat IgG2a antibody, FITC (filled histogram), or a rat IgG2a, kappa isotype control antibody followed by a mouse anti-rat IgG2a antibody, FITC (solid line histogram).

Figure 2. Data for APC-Conjugated

Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD19 Antibody, Clone 1D3, APC (filled histogram) or a rat IgG2a, kappa isotype control antibody, APC (solid line histogram).

Figure 3. Data for Biotin-Conjugated

Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD19 Antibody, Clone 1D3, Biotin, followed by streptavidin (SAV) APC (filled histogram), or a biotinylated rat IgG2a, kappa isotype control antibody followed by SAV APC (solid line histogram).

Figure 4. Data for FITC-Conjugated

Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD19 Antibody, Clone 1D3, FITC (filled histogram) or a rat IgG2a, kappa isotype control antibody, FITC (solid line histogram).

Figure 5. Data for PE-Conjugated

Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD19 Antibody, Clone 1D3, PE (filled histogram) or a rat IgG2a, kappa isotype control antibody, PE (solid line histogram).

Figure 6. Data for PerCP-Cy55-Conjugated

Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD19 Antibody, Clone 1D3, PerCP-Cy5.5 (filled histogram) or a rat IgG2a, kappa isotype control antibody, PerCP-Cy5.5 (solid line histogram).