抗小鼠CD4抗体，克隆RM4-4

抗小鼠CD4的大鼠单克隆IgG2b抗体

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产品号 #（选择产品）

产品号 #60029_C

抗小鼠CD4的大鼠单克隆IgG2b抗体

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总览

实验数据

产品说明书及文档

应用领域

总览

RM4-4抗体可特异性识别小鼠CD4。CD4是一种分子量约为55 kDa的单链1型跨膜糖蛋白，属于免疫球蛋白 (Ig) 超家族成员；CD4包含4个细胞外Ig样结构域。CD4在大多数胸腺细胞和T细胞亚群（T辅助性/诱导细胞）中表达水平相对较高，而在树突状细胞中表达水平较低。小鼠的单核细胞/巨噬细胞不表达CD4。CD4与MHC II的非多态性区域结合，并通过增强TCR与MHC II-抗原复合物结合的亲和力，在MHC II限制性抗原识别中作为T细胞受体（TCR）的共受体。CD4还通过其胞内结构域与胞质酪氨酸激酶（如Lck）的相互作用，将信号从TCR放大至细胞质。RM4-4抗体的结合不会阻断抗体克隆RM4-5或GK1.5的结合。

亚型
一抗

靶抗原
CD4

别名
L3T4，T4

活性物种
小鼠

偶联
APC，Biotin 或生物素，FITC，PE，未偶联的

宿主物种
大鼠

细胞类型
T 细胞，T 细胞，CD4+，T 细胞，调节性细胞

种属
小鼠

应用
流式细胞术，功能学筛选

研究领域
免疫

克隆
RM4-4

基因编号
12504

同种型
IgG2b，kappa

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实验数据

Figure 1. Data for Unconjugated

Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD4 Antibody, Clone RM4-4, followed by a mouse anti-rat IgG2b antibody, FITC (filled histogram), or Rat IgG2b, kappa Isotype Control Antibody, Clone RTK4530 (Catalog #60077), followed by a mouse anti-rat IgG2b antibody, FITC (solid line histogram).

Figure 2. Data for APC-Conjugated

Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD4 Antibody, Clone RM4-4, APC (filled histogram) or Rat IgG2b, kappa Isotype Control Antibody, Clone RTK4530, APC (Catalog #60077AZ) (solid line histogram).

Figure 3. Data for Biotin-Conjugated

Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD4 Antibody, Clone RM4-4, Biotin, followed by streptavidin (SAV) APC (filled histogram), or Rat IgG2b, kappa Isotype Control Antibody, Clone RTK4530, Biotin (Catalog #60077BT), followed by SAV APC (solid line histogram).

Figure 4. Data for FITC-Conjugated

Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD4 Antibody, Clone RM4-4, FITC (filled histogram) or Rat IgG2b, kappa Isotype Control Antibody, Clone RTK4530, FITC (Catalog #60077FI) (solid line histogram).

Figure 5. Data for PE-Conjugated

Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD4 Antibody, Clone RM4-4, PE (filled histogram) or Rat IgG2b, kappa Isotype Control Antibody, Clone RTK4530, PE (Catalog #60077PE) (solid line histogram).