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抗小鼠CD45抗体，克隆30-F11

大鼠单克隆IgG2b抗体，抗小鼠CD45
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产品号 #（选择产品）

产品号 #60030_C

大鼠单克隆IgG2b抗体，抗小鼠CD45

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总览
实验数据
产品说明书及文档
应用领域
相关材料与文献
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总览

30-F11抗体识别小鼠CD45的所有亚型和同种异构抗原（CD45.1和CD45.2）上的一个细胞外表位。CD45是一种I型跨膜糖蛋白，在大多数血细胞表面表达，除了成熟红细胞、血小板和浆细胞外（这些细胞类型在分化过程中会丢失CD45表达）。CD45是蛋白酪氨酸磷酸酶家族的一员，在许多免疫调节过程中起作用，包括细胞活化、生长、分化和致癌转化。CD45的大细胞质部分包含两个酪氨酸磷酸酶结构域（其中一个具有酶活性），参与调节细胞内底物（如Src激酶Lck和Fyn）的功能。CD45的几种亚型已经被鉴定出来，其表达根据细胞类型和功能状态而不同。编码胞外RA， RB和RC多肽序列的三个外显子（4,5,6）的选择性剪接产生了多达8个分子量在180 - 240 kDa范围内的异构体。

亚型
一抗
 
靶抗原
CD45
 
别名
LCA，白细胞共同抗原，Ly-5，C型蛋白酪氨酸磷酸酶受体，PTPRC，T200
 
活性物种
小鼠
 
偶联
Alexa Fluor 488，APC，Biotin 或 生物素，FITC，PE，PerCP-Cyanine5.5，未偶联的
 
宿主物种
大鼠
 
细胞类型
粒细胞及其亚群，造血干/祖细胞，淋巴细胞，髓系细胞
 
种属
小鼠
 
应用
CyTOF，流式细胞术，功能学筛选，免疫细胞化学，免疫荧光，免疫组化，免疫沉淀，Western印迹
 
研究领域
免疫
 
克隆
30-F11
 
基因编号
19264
 
同种型
IgG2b，kappa
 

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实验数据

Data for Unconjugated

Figure 1. Data for Unconjugated

Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD45 Antibody, Clone 30-F11, followed by a mouse anti-rat IgG2b antibody, FITC (filled histogram), or Rat IgG2b, kappa Isotype Control Antibody, Clone RTK4530 (Catalog #60077), followed by a mouse anti-rat IgG2b antibody, FITC (solid line histogram).

Data for Alexa Fluor® 488-Conjugated

Figure 2. Data for Alexa Fluor® 488-Conjugated

Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD45 Antibody, Clone 30-F11, Alexa Fluor® 488 (filled histogram) or Rat IgG2b, kappa Isotype Control Antibody, Clone RTK4530, Alexa Fluor® 488 (Catalog #60077AD) (solid line histogram).

Data for APC-Conjugated

Figure 3. Data for APC-Conjugated

Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD45 Antibody, Clone 30-F11, APC (filled histogram) or Rat IgG2b, kappa Isotype Control Antibody, Clone RTK4530, APC (Catalog #60077AZ) (solid line histogram).

Data for Biotin-Conjugated

Figure 4. Data for Biotin-Conjugated

Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD45 Antibody, Clone 30-F11, Biotin, followed by streptavidin (SAV) APC (filled histogram), or Rat IgG2b, kappa Isotype Control Antibody, Clone RTK4530, Biotin (Catalog #60077BT), followed by SAV APC (solid line histogram).

Data for FITC-Conjugated

Figure 5. Data for FITC-Conjugated

Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD45 Antibody, Clone 30-F11, FITC (filled histogram) or Rat IgG2b, kappa Isotype Control Antibody, Clone RTK4530, FITC (Catalog #60077FI) (solid line histogram).

Data for PE-Conjugated

Figure 6. Data for PE-Conjugated

Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD45 Antibody, Clone 30-F11, PE (filled histogram) or Rat IgG2b, kappa Isotype Control Antibody, Clone RTK4530, PE (Catalog #60077PE) (solid line histogram).

Data for PerCP-Cy55-Conjugated

Figure 7. Data for PerCP-Cy55-Conjugated

Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD45 Antibody, Clone 30-F11, PerCP-Cy5.5 (filled histogram) or a rat IgG2b, kappa PerCP-Cy5.5 isotype control antibody (solid line histogram).

产品说明书及文档

请在《产品说明书》中查找相关支持信息和使用说明，或浏览下方更多实验方案。

Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet
Product Name
Anti-Mouse CD45 Antibody, Clone 30-F11, Alexa Fluor® 488
Catalog #
60030AD, 60030AD.1
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Mouse CD45 Antibody, Clone 30-F11, APC
Catalog #
60030AZ, 60030AZ.1
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Mouse CD45 Antibody, Clone 30-F11, Biotin
Catalog #
60030BT.1, 60030BT
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Mouse CD45 Antibody, Clone 30-F11, FITC
Catalog #
60030FI.1
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Mouse CD45 Antibody, Clone 30-F11, PE
Catalog #
60030PE, 60030PE.1
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Mouse CD45 Antibody, Clone 30-F11, PerCP-Cy5.5
Catalog #
60030PS, 60030PS.1
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Mouse CD45 Antibody, Clone 30-F11
Catalog #
60030.1, 60030
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Mouse CD45 Antibody, Clone 30-F11, Alexa Fluor® 488
Catalog #
60030AD, 60030AD.1
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Mouse CD45 Antibody, Clone 30-F11, APC
Catalog #
60030AZ, 60030AZ.1
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Mouse CD45 Antibody, Clone 30-F11, Biotin
Catalog #
60030BT.1, 60030BT
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Mouse CD45 Antibody, Clone 30-F11, FITC
Catalog #
60030FI.1
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Mouse CD45 Antibody, Clone 30-F11, PE
Catalog #
60030PE, 60030PE.1
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Mouse CD45 Antibody, Clone 30-F11, PerCP-Cy5.5
Catalog #
60030PS, 60030PS.1
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Mouse CD45 Antibody, Clone 30-F11
Catalog #
60030.1, 60030
Lot #
All
Language
English

应用领域

本产品专为以下研究领域设计，适用于工作流程中的高亮阶段。探索这些工作流程，了解更多我们为各研究领域提供的其他配套产品。

Research Area
Workflow Stages

更多信息

更多信息
种属 Mouse
克隆 30-F11
Gene Id 19264
Alternative Names LCA, Leukocyte common antigen, Ly-5, Protein tyrosine phosphatase receptor type C, PTPRC, T200
同种型/isotype IgG2b, kappa
法律声明:

Alexa Fluor is a registered trademark of Life Technologies Corporation. Antibodies conjugated to Alexa Fluor® are licensed for internal research use only and sale is expressly conditioned on the buyer not using the antibody for manufacturing, performing a service or medical test, or otherwise generating revenue. For use other than research, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com.

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