抗小鼠Gr-1抗体，克隆RB6-8C5

大鼠单克隆IgG2b抗体，抗小鼠Gr-1 （Ly-6G/Ly-6C）

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产品号 #60028_C

大鼠单克隆IgG2b抗体，抗小鼠Gr-1 （Ly-6G/Ly-6C）

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总览

实验数据

产品说明书及文档

应用领域

总览

RB6-8C5抗体识别结构上相关的小鼠Ly-6G （21 - 25 kDa）和Ly-6C（14 - 16 kDa）GPI锚定蛋白，这两种蛋白共同构成粒细胞受体-1抗原（Gr-1）。Gr-1在单核细胞、中性粒细胞、巨噬细胞的亚群、浆细胞样树突状细胞和T细胞上表达。骨髓单核细胞在发育过程中短暂表达Gr-1，其表达水平与粒细胞分化和成熟密切相关。在外周，Gr-1主要存在于中性粒细胞上，是中性粒细胞的实用标志物。RB6-8C5既可以与Ly-6G又可以与Ly-6C结合，而另一种常用的抗Gr-1抗体克隆1A8只能与Ly-6G特异性结合。据报导，1A8抗体可用于检测外周血中表达Ly-6G的粒细胞，而RB6-8C5抗体也可以结合表达Ly-6C的淋巴细胞、单核细胞和树突状细胞。

该抗体克隆已被验证可用于评估EasySep™试剂盒（包括EasySep™小鼠中性粒细胞富集试剂盒（产品号 #19762））分选的细胞纯度。

亚型
一抗

靶抗原
Gr-1 (Ly-6G/Ly-6C)

别名
Gr 1，Gr1，Ly 6G，Ly6G，Ly-6G/Ly-6C

活性物种
小鼠

偶联
Alexa Fluor 488，APC，Biotin 或生物素，FITC，PE，PerCP-Cyanine5.5，未偶联的

宿主物种
大鼠

细胞类型
粒细胞及其亚群，单核细胞，髓系细胞

种属
小鼠

应用
细胞分选，流式细胞术，免疫细胞化学，免疫沉淀，Western印迹

研究领域
免疫

克隆
RB6-8C5

基因编号
17067/546644

同种型
IgG2b，kappa

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实验数据

Figure 1. Data for Alexa Fluor® 488-Conjugated

Flow cytometry analysis of C57BL/6 mouse bone marrow cells labeled with Anti-Mouse Gr-1 Antibody, Clone RB6-8C5, Alexa Fluor® 488 (filled histogram) or a rat IgG2b, kappa Alexa Fluor® 488 isotype control antibody (solid line histogram).

Figure 2. Data for Biotin-Conjugated

Flow cytometry analysis of C57BL/6 mouse bone marrow cells labeled with Anti-Mouse Gr-1 Antibody, Clone RB6-8C5, Biotin, followed by streptavidin (SAV) APC (filled histogram), or a biotinylated rat IgG2b, kappa isotype control antibody, followed by SAV APC (solid line histogram).

Figure 3. Data for PE-Conjugated

Flow cytometry analysis of C57BL/6 mouse bone marrow cells labeled with Anti-Mouse Gr-1 Antibody, Clone RB6-8C5, PE (filled histogram) or a rat IgG2b, kappa PE isotype control antibody (solid line histogram).

Figure 4. Data for Unconjugated

Flow cytometry analysis of C57BL/6 mouse bone marrow cells labeled with Anti-Mouse Gr-1 Antibody, Clone RB6-8C5, followed by Goat Anti-Mouse IgG (H+L) Antibody, Polyclonal, FITC (Catalog #60138FI) (filled histogram), or a rat IgG2b, kappa isotype control antibody, followed by Goat Anti-Mouse IgG (H+L) Antibody, Polyclonal, FITC is shown (solid line histogram).

Figure 5. Data for APC-Conjugated

Flow cytometry analysis of C57BL/6 mouse bone marrow cells labeled with Anti-Mouse Gr-1 Antibody, Clone RB6-8C5, APC (filled histogram) or Rat IgG2b, kappa Isotype Control Antibody, Clone RTK4530, APC (Catalog #60077AZ) (solid line histogram).

Figure 6. Data for FITC-Conjugated

Flow cytometry analysis of C57BL/6 mouse bone marrow cells labeled with Anti-Mouse Gr-1 Antibody, Clone RB6-8C5, FITC (filled histogram) or Rat IgG2b, kappa Isotype Control Antibody, Clone RTK4530, FITC (Catalog #60077FI) (solid line histogram).

Figure 7. Data for PerCP-Cy5.5-Conjugated

Flow cytometry analysis of C57BL/6 mouse splenocytes (gated on granulocytes) labeled with Anti-Mouse Gr-1 Antibody, Clone RB6-8C5, PerCP-Cy5.5 (filled histogram) or a rat IgG2b, kappa isotype control antibody, PerCP-Cy5.5 (solid line histogram).