抗小鼠Ly-6G抗体，克隆1A8

大鼠单克隆IgG2a抗体，抗小鼠Ly-6G

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产品号 #（选择产品）

产品号 #60031_C

大鼠单克隆IgG2a抗体，抗小鼠Ly-6G

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总览

实验数据

产品说明书及文档

应用领域

总览

1A8抗体识别Ly-6G，Ly-6G是一种21 - 25 kDa的GPI锚定蛋白，与结构上相关的Ly-6C蛋白一起组成粒细胞受体-1抗原（Gr-1）。Gr-1在单核细胞、中性粒细胞、巨噬细胞的亚群、浆细胞样树突状细胞和T细胞上表达。骨髓单核细胞在发育过程中短暂表达Gr-1，其表达水平与粒细胞分化和成熟密切相关。在外周，Gr-1主要存在于中性粒细胞上，是中性粒细胞的实用标志物。1A8抗体特异性地与Ly-6G结合，而另一种常用的抗体克隆RB6-8C5，既可以与Ly-6G又可以与Ly-6C结合。据报导，1A8抗体可用于检测外周血中表达Ly-6G的粒细胞，而RB6-8C5抗体也可以结合表达Ly-6C的淋巴细胞、单核细胞和树突状细胞。

该抗体克隆已被验证可用于评估EasySep™试剂盒（包括EasySep™小鼠中性粒细胞富集试剂盒（产品号 #19762））分选的细胞纯度。

亚型
一抗

靶抗原
Ly-6G

别名
Gr-1，GR1，Ly6G

活性物种
小鼠

偶联
APC，Biotin 或生物素，FITC，Pacific Blue，PE，PerCP-Cyanine5.5，未偶联的

宿主物种
大鼠

细胞类型
粒细胞及其亚群，髓系细胞

种属
小鼠

应用
流式细胞术，功能学筛选，免疫细胞化学，免疫沉淀

研究领域
免疫

克隆
1A8

基因编号
546644

同种型
IgG2a，kappa

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实验数据

Figure 1. Data for FITC-Conjugated

Flow cytometry analysis of C57BL/6 mouse bone marrow cells labeled with Anti-Mouse Ly-6G Antibody, Clone 1A8, FITC (filled histogram) or a rat IgG2a, kappa FITC isotype control antibody (solid line histogram).

Figure 2. Data for PE-Conjugated

Flow cytometry analysis of C57BL/6 mouse bone marrow cells labeled with Anti-Mouse Ly-6G Antibody, Clone 1A8, PE (filled histogram) or a rat IgG2a, kappa PE isotype control antibody (solid line histogram).

Figure 3. Data for Unconjugated

(A) Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse Ly-6G Antibody, Clone 1A8, followed by a mouse anti-rat IgG2a antibody, FITC (filled histogram), or Rat IgG2a, kappa Isotype Control Antibody, Clone RTK2758 (Catalog #60076), followed by a mouse anti-rat IgG2a antibody, FITC (solid line histogram). (B) Immunohistochemical analysis of formalin-fixed, paraffin-embedded mouse uterine tissue labeled with Anti-Mouse Ly-6G Antibody, Clone 1A8, followed by anti-mouse, HRP. DAB substrate was used for visualization.

Figure 4. Data for APC-Conjugated

Flow cytometry analysis of C57BL/6 mouse bone marrow cells labeled with Anti-Mouse Ly-6G Antibody, Clone 1A8, APC (filled histogram) or Rat IgG2a, kappa Isotype Control Antibody, Clone RTK2758, APC (Catalog #60076AZ) (solid line histogram).

Figure 5. Data for Biotin-Conjugated

Flow cytometry analysis of C57BL/6 mouse bone marrow cells labeled with Anti-Mouse Ly-6G Antibody, Clone 1A8, Biotin, followed by streptavidin (SAV) APC (filled histogram), or Rat IgG2a, kappa Isotype Control Antibody, Clone RTK2758, Biotin (Catalog #60076BT), followed by SAV APC (solid line histogram).

Figure 6. Data for PerCP-Cy55-Conjugated

Flow cytometry analysis of C57BL/6 mouse bone marrow cells labeled with Anti-Mouse Ly-6G Antibody, Clone 1A8, PerCP-Cy5.5 (filled histogram) or a rat IgG2a, kappa isotype control antibody, PerCP-Cy5.5 (solid line histogram).

Figure 7. Data for PB-Conjugated

Flow cytometry analysis of C57BL/6 mouse bone marrow cells labeled with Anti-Mouse Ly-6G Antibody, Clone 1A8, Pacific Blue™ (filled histogram) or a rat IgG2a, kappa Pacific Blue™ isotype control antibody (solid line histogram).