产品号 #100-0277_C
抗小鼠IFN-gamma的大鼠单克隆IgG1抗体
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XMG1.2抗体与小鼠干扰素γ (IFN-γ)发生反应,IFN-γ是一种糖基化的II型干扰素,其活性形式包含约20 kDa亚基的同型二聚体。IFN‑γ 主要由活化的 T 淋巴细胞和自然杀伤(NK)细胞在抗原刺激下产生,这些抗原可包括同种异体抗原、有丝分裂原及肠毒素等。IFN-γ与IFN-γ受体的α亚基具有高亲和力结合,导致配体诱导的四聚体α2β2受体复合物的组装,该复合物能够通过激活JAK-STAT途径介导细胞对细胞因子的反应。IFN-γ受体在所有有核细胞表面表达。IFN-γ在多种生物过程中发挥重要功能,包括巨噬细胞活化,介导对病毒和细菌的免疫反应,以及调节细胞增殖和凋亡。当与适当的二抗配对时,XMG1.2抗体适用于流式细胞术等方法检测细胞内IFN-γ。XMG1.2抗体也被广泛用于各种检测中,以中和IFN-γ的功能活性。
亚型
一抗
靶抗原
IFNγ
别名
IFN-γ,免疫干扰素,干扰素-γ,巨噬细胞活化因子(MAF),T细胞干扰素,II型干扰素
活性物种
小鼠
偶联
APC,Biotin 或 生物素,FITC,PE
宿主物种
大鼠
细胞类型
树突状细胞(DCs),巨噬细胞,NK 细胞,T 细胞
应用
ELISAs,ELISpot,流式细胞术
研究领域
免疫
克隆
XMG1.2
基因编号
15978
同种型
IgG1,kappa
Figure 1. Data for Anti-Mouse IFN-gamma Antibody, Clone XMG1.2, APC
Flow cytometry analysis of (A) unstimulated C57BL/6 mouse splenocytes labeled with Anti-Mouse IFN-gamma Antibody, Clone XMG1.2, APC, and anti-mouse CD3e antibody, clone 145-2C11, PE and (B) PMA/ionomycin-stimulated C57BL/6 mouse splenocytes labeled with Anti-Mouse IFN-gamma Antibody, Clone XMG1.2, APC, and anti-mouse CD3e antibody, clone 145-2C11, PE.
Figure 2. Data for Anti-Mouse IFN-gamma Antibody, Clone XMG1.2, Biotin
Flow cytometry analysis of PMA/ionomycin-stimulated C57BL/6 mouse splenocytes labeled with Anti-Mouse IFN-γ Antibody, Clone XMG1.2, Biotin, followed by streptavidin (SAV) PE.
Figure 3. Data for Anti-Mouse IFN-gamma Antibody, Clone XMG1.2, FITC
Flow cytometry analysis of PMA/ionomycin-stimulated C57BL/6 mouse splenocytes labeled with Anti-Mouse IFN-gamma Antibody, Clone XMG1.2, FITC.
Figure 4. Data for Anti-Mouse IFN-gamma Antibody, Clone XMG1.2, PE
Flow cytometry analysis of (A) unstimulated C57BL/6 mouse splenocytes labeled with Anti-Mouse IFN-gamma Antibody, Clone XMG1.2, PE, and anti-mouse CD3e antibody, clone 145-2C11, APC and (B) PMA/ionomycin-stimulated C57BL/6 mouse splenocytes labeled with Anti-Mouse IFN-gamma Antibody, Clone XMG1.2, PE, and anti-mouse CD3e antibody, clone 145-2C11, APC.
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克隆 | XMG1.2 |
---|---|
Gene Id | 15978 |
Alternative Names | IFN-γ, Immune interferon, Interferon-gamma, Macrophage-activating factor (MAF), T cell interferon, Type II interferon |
同种型/isotype | IgG1, kappa |
用于小鼠干扰素γ的检测和测量
小鼠naïve CD4+ T细胞向Th1细胞分化的无血清培养补充
小鼠Naïve CD4+ T细胞的免疫磁珠负选
干扰素-γ
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