STEMdiff™ 感觉神经元分化试剂盒

用于从人 iPS 细胞衍生的神经嵴细胞生成感觉神经元前体的分化试剂盒

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产品号 #（选择产品）

产品号 #100-0341_C

用于从人 iPS 细胞衍生的神经嵴细胞生成感觉神经元前体的分化试剂盒

产品优势

通过消除昂贵的背根神经节外植体工作流程来减少动物使用
使用 NCC 中间体和 STEMdiff™ 神经嵴分化试剂盒，从多个人类诱导多能干细胞 (iPS) 系中分化出感觉神经元
通过简单易用的培养基格式简化 PSC 衍生的感觉神经元生成
通过集成 BrainPhys™ 培养基支持神经元活动和成熟，获得生理相关结果

产品组分包括

STEMdiff™ 感觉神经元分化基础培养基，100 mL
STEMdiff™ 感觉神经元分化补充剂，1 mL

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Need a high-quality cell source? Use the hiPSC SCTi003-A (female) or SCTi004-A (male) control lines, manufactured with mTeSR™ Plus.

要查看实验方案所需的所有配套产品，请参阅《实验方案与技术文档》。

STEMdiff™ Neural Crest Differentiation Kit
Cell culture kit for establishment of hPSC-derived neural crest cells
STEMdiff™ Sensory Neuron Maturation Kit
Maturation kit for generation of functional sensory neurons from human iPS cell-derived sensory neuronal precursor cells
Labeling Antibodies
Compatible antibodies for purity assessment of isolated cells

总览

实验数据

产品说明书及文档

应用领域

总览

用这个易于使用的培养系统在培养皿中产生功能性的、人类特异性的感觉神经元。使用STEMdiff™神经嵴分化试剂盒获得多能干细胞（PSC）来源的神经嵴细胞（NCCs）后，使用无血清STEMdiff™感觉神经元分化试剂盒生成感觉神经元前体。STEMdiff™感觉神经元成熟试剂盒可用于维持和成熟感觉神经元前体。得到的细胞brn3a阳性，超过70%的tuj1阳性。以BrainPhys™提供的生理葡萄糖和渗透压条件为基础培养基，神经元表现出对感觉配体和温度变化的反应活性。生成的功能性人类感觉神经元群可用于药物发现和疼痛研究应用。

亚型
专用培养基

细胞类型
神经细胞，PSC衍生，神经元

种属
人

应用
细胞培养，分化

品牌
BrainPhys，STEMdiff

研究领域
疾病建模，药物发现和毒理检测，神经科学

制剂类别
无血清

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实验数据

Experimental Protocol for STEMdiff™ Sensory Neuron Differentiation and Maturation Kits

Figure 1. Schematic for the STEMdiff™ Sensory Neuron Culture System Protocol

Sensory neuron precursors can be generated in 6 days from hPSC-derived neural crest cells. For the generation of neural crest cells, see documentation for STEMdiff™ Neural Crest Differentiation Kit (Catalog #08610). For the maturation of sensory neuron precursors to sensory neurons, see the PIS.

Figure 2. STEMdiff™ Sensory Neuron Kits Promote Differentiation Across Multiple Embryonic Stem and Induced Pluripotent Stem Cell Lines

NCCs generated from hPSCs in mTeSR™ Plus using the STEMdiff™ Neural Crest Differentiation Kit were differentiated and matured to sensory neurons using the STEMdiff™ Sensory Neuron Differentiation and Maturation Kits. (A) Sensory neurons were generated after hPSC-derived NCCs were cultured with the STEMdiff™ Sensory Neuron Differentiation Kit for 6 days and then the STEMdiff™ Sensory Neuron Maturation Kit for 6 days. The resulting cultures contain a population of cells expressing sensory neuron markers peripherin (green) and BRN3A (red) along with (B) neuronal marker class III β-tubulin (TUJ1, red). (C) Midbrain neuron controls generated with STEMdiff™ Midbrain Neuron Differentiation and Maturation Kits do not have detectable peripherin (green) or BRN3A (red) expression, although they express (D) neuronal marker class III β-tubulin (TUJ1, red). Nuclei are labeled with DAPI (blue). Human ES and iPS cell lines were maintained in either mTeSR™1, TeSR™-E8™, or mTeSR™ Plus and differentiated with STEMdiff™ Neural Crest Differentiation Kit, followed by STEMdiff™ Sensory Neuron Differentiation and Maturation Kits. The percentage expression of (E) BRN3A+ and (F) TUJ1+ cells in the resulting cultures was quantified. This differentiation generated BRN3A+ sensory neurons (25.3% ± 6.9%, mean ± SEM; n=7 cell lines, 3 - 23 replicates per condition) that expressed neuronal marker class III β-tubulin (TUJ1; 90.3% ± 4.1%, mean ± SEM; n=4 cell lines, 3 - 12 replicates per condition). Numbers are % positive over total DAPI in a tiled image. NCCs = neural crest cells; hPSCs = human pluripotent stem cells; ES = embryonic stem; iPS = induced pluripotent stem