STEMdiff™ 感觉神经元成熟试剂盒

用于从人iPS细胞衍生的感觉神经元前体细胞生成功能性感觉神经元的成熟试剂盒

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产品号 #（选择产品）

产品号 #100-0684_C

用于从人iPS细胞衍生的感觉神经元前体细胞生成功能性感觉神经元的成熟试剂盒

产品优势

通过省去昂贵的背根神经节组织外植流程，减少动物使用
在使用 STEMdiff™ 感觉神经元分化试剂盒后，顺利实现感觉神经元的成熟与维持
简单易用的培养基格式，简化由多能干细胞生成感觉神经元的流程
集成 BrainPhys™ 培养基，支持神经元活性和成熟，获得更符合生理特性的实验结果

产品组分包括

BrainPhys™ 神经元培养基，100 mL（目录号 #05797）
STEMdiff™ 感觉神经元成熟补充剂，25 mL

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要查看实验方案所需的所有配套产品，请参阅《实验方案与技术文档》。

STEMdiff™ Neural Crest Differentiation Kit
Cell culture kit for establishment of hPSC-derived neural crest cells
STEMdiff™ Sensory Neuron Differentiation Ki...
Differentiation kit for the generation of sensory neuronal precursors from human iPS cell-derived neural crest cells
Labeling Antibodies
Compatible antibodies for purity assessment of isolated cells

总览

实验数据

产品说明书及文档

应用领域

总览

使用这种易于使用的培养系统在培养皿中生成功能性的、人类特异性的感觉神经元。STEMdiff™ 感觉神经元成熟试剂盒是从人多能干细胞 (hPSC) 开始，最终生成可用于药物研发和疼痛研究应用的感觉神经元的工作流程的最后一步。使用STEMdiff™ 神经嵴分化试剂盒获得 PSC 衍生的神经嵴细胞 (NCC) 后，可使用无血清 STEMdiff™ 感觉神经元分化试剂盒生成感觉神经元前体细胞。然后，可以使用 STEMdiff™ 感觉神经元成熟试剂盒来维持和成熟感觉神经元前体细胞。最终获得的细胞中，BRN3A 阳性，且超过 70% 为 TUJ1 阳性。以 BrainPhys™ 为基础培养基，提供生理水平的葡萄糖和渗透压条件，使得成熟神经元能对感觉配体及温度变化作出反应，展现出功能活性。

亚型
专用培养基

细胞类型
神经细胞，PSC衍生，神经元

种属
人

应用
细胞培养，鉴定，分化，功能学筛选，免疫细胞化学，表型鉴定

品牌
BrainPhys，STEMdiff

研究领域
疾病建模，药物发现和毒理检测，神经科学

制剂类别
无血清

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实验数据

Experimental Protocol for STEMdiff™ Sensory Neuron Differentiation and Maturation Kits

Figure 1. Schematic for the STEMdiff™ Sensory Neuron Culture System Protocol

Sensory neurons can be generated in 7 days from sensory neuron precursors after 6 days in STEMdiff™ Sensory Neuron Differentiation Medium. For the differentiation of sensory neuron precursors from hPSC-derived neural crest cells, see the PIS.

Figure 2. STEMdiff™ Sensory Neuron Kits Promote Differentiation Across Multiple Embryonic Stem and Induced Pluripotent Stem Cell Lines

NCCs generated from hPSCs in mTeSR™ Plus using the STEMdiff™ Neural Crest Differentiation Kit were differentiated and matured to sensory neurons using the STEMdiff™ Sensory Neuron Differentiation and Maturation Kits. (A) Sensory neurons were generated after hPSC-derived NCCs were cultured with the STEMdiff™ Sensory Neuron Differentiation Kit for 6 days and then the STEMdiff™ Sensory Neuron Maturation Kit for 6 days. The resulting cultures contain a population of cells expressing sensory neuron markers peripherin (green) and BRN3A (red) along with (B) neuronal marker class III β-tubulin (TUJ1, red). (C) Midbrain neuron controls generated with STEMdiff™ Midbrain Neuron Differentiation and Maturation Kits do not have detectable peripherin (green) or BRN3A (red) expression, although they express (D) neuronal marker class III β-tubulin (TUJ1, red). Nuclei are labeled with DAPI (blue). Human ES and iPS cell lines were maintained in either mTeSR™1, TeSR™-E8™, or mTeSR™ Plus and differentiated with STEMdiff™ Neural Crest Differentiation Kit, followed by STEMdiff™ Sensory Neuron Differentiation and Maturation Kits. The percentage expression of (E) BRN3A+ and (F) TUJ1+ cells in the resulting cultures was quantified. This differentiation generated BRN3A+ sensory neurons (25.3% ± 6.9%, mean ± SEM; n=7 cell lines, 3 - 23 replicates per condition) that expressed neuronal marker class III β-tubulin (TUJ1; 90.3% ± 4.1%, mean ± SEM; n=4 cell lines, 3 - 12 replicates per condition). Numbers are % positive over total DAPI in a tiled image. NCCs = neural crest cells; hPSCs = human pluripotent stem cells; ES = embryonic stem; iPS = induced pluripotent stem