搜索结果: 'methocult media formulations for mouse hematopoietic cells serum containing'

Reference

Lam BS et al. (JAN 2011) Blood 117 4 1167--75

Pharmacologic modulation of the calcium-sensing receptor enhances hematopoietic stem cell lodgment in the adult bone marrow.

The ability of hematopoietic stem cells (HSCs) to undergo self-renewal is partly regulated by external signals originating from the stem cell niche. Our previous studies with HSCs obtained from fetal liver of mice deficient for the calcium-sensing receptor (CaR) have shown the crucial role of this receptor in HSC lodgment and engraftment in the bone marrow (BM) endosteal niche. Using a CaR agonist,Cinacalcet,we assessed the effects of stimulating the CaR on the function of murine HSCs. Our results show that CaR stimulation increases primitive hematopoietic cell activity in vitro,including growth in stromal cell cocultures,adhesion to extracellular matrix molecules such as collagen I and fibronectin,and migration toward the chemotactic stimulus,stromal cell-derived factor 1α. Receptor stimulation also led to augmented in vivo homing,CXCR4-mediated lodgment at the endosteal niche,and engraftment capabilities. These mechanisms by which stimulating the CaR dictates preferential localization of HSCs in the BM endosteal niche provide additional insights into the fundamental interrelationship between the stem cell and its niche. These studies also have implications in the area of clinical stem cell transplantation,where ex vivo modulation of the CaR may be envisioned as a strategy to enhance HSC engraftment in the BM. View Publication

产品类型：

产品号#：

03434

03444

产品名：

MethoCult™GF M3434

Reference

Shimakura Y et al. (JAN 2000) Stem cells (Dayton,Ohio) 18 3 183--9

Murine stromal cell line HESS-5 maintains reconstituting ability of Ex vivo-generated hematopoietic stem cells from human bone marrow and cytokine-mobilized peripheral blood.

Human bone marrow (BM) or mobilized peripheral blood (mPB) CD34(+) cells have been shown to loose their stem cell quality during culture period more easily than those from cord blood (CB). We previously reported that human umbilical CB stem cells could effectively be expanded in the presence of human recombinant cytokines and a newly established murine bone marrow stromal cell line HESS-5. In this study we assessed the efficacy of this xenogeneic coculture system using human BM and mPB CD34(+) cells as materials. We measured the generation of CD34(+)CD38(-) cells and colony-forming units,and assessed severe-combined immunodeficient mouse-repopulating cell (SRC) activity using cells five days after serum-free cytokine-containing culture in the presence or the absence of a direct contact with HESS-5 cells. As compared with the stroma-free culture,the xenogeneic coculture was significantly superior on expansion of CD34(+)CD38(-) cells and colony-forming cells and on maintenance of SRC activity. The PKH26 study demonstrated that cell division was promoted faster in cells cocultured with HESS-5 cells than in cells cultured without HESS-5 cells. These results indicate that HESS-5 supports rapid generation of primitive progenitor cells (PPC) and maintains reconstituting ability of newly generated stem cells during ex vivo culture irrespective of the source of samples. This xenogeneic coculture system will be useful for ex vivo manipulation such as gene transduction to promote cell division and the generation of PPC and to prevent loss of stem cell quality. View Publication

产品类型：

产品号#：

04064

04034

04044

产品名：

入门套件的MethoCult™H4034优化

MethoCult™H4034 Optimum

Reference

Pirson L et al. (JUL 2006) Stem cells (Dayton,Ohio) 24 7 1814--21

Despite inhibition of hematopoietic progenitor cell growth in vitro, the tyrosine kinase inhibitor imatinib does not impair engraftment of human CD133+ cells into NOD/SCIDbeta2mNull mice.

There is potential interest for combining allogeneic hematopoietic cell transplantation (HCT),and particularly allogeneic HCT with a nonmyeloablative regimen,to the tyrosine kinase inhibitor imatinib (Glivec; Novartis,Basel,Switzerland,http://www.novartis.com) in order to maximize anti-leukemic activity against Philadelphia chromosome-positive leukemias. However,because imatinib inhibits c-kit,the stem cell factor receptor,it could interfere with bone marrow engraftment. In this study,we examined the impact of imatinib on normal progenitor cell function. Imatinib decreased the colony-forming capacity of mobilized peripheral blood human CD133(+) cells but not that of long-term culture-initiating cells. Imatinib also decreased the proliferation of cytokine-stimulated CD133(+) cells but did not induce apoptosis of these cells. Expression of very late antigen (VLA)-4,VLA-5,and CXCR4 of CD133(+) cells was not modified by imatinib,but imatinib decreased the ability of CD133(+) cells to migrate. Finally,imatinib did not decrease engraftment of CD133(+) cells into irradiated nonobese diabetic/severe combined immunodeficient/beta2m(null) mice conditioned with 3 or 1 Gy total body irradiation. In summary,our results suggest that,despite inhibition of hematopoietic progenitor cell growth in vitro,imatinib does not interfere with hematopoietic stem cell engraftment. View Publication

产品类型：

产品号#：

05150

04435

04445

04960

04902

04900

04961

04901

04963

04962

04970

04971

产品名：

MyeloCult™H5100

MethoCult™H4435富集

MegaCult™-C胶原蛋白和不含细胞因子的培养基

胶原蛋白溶液

MegaCult™-C培养基无细胞因子

MegaCult™-C胶原蛋白和细胞因子培养基

MegaCult™-C细胞因子培养基

双室载玻片试剂盒

MegaCult™-C cfu染色试剂盒

MegaCult™-C不含细胞因子完整试剂盒

MegaCult™-C细胞因子完整试剂盒

Reference

Andreani M et al. (JAN 2011) Haematologica 96 1 128--33

Quantitatively different red cell/nucleated cell chimerism in patients with long-term, persistent hematopoietic mixed chimerism after bone marrow transplantation for thalassemia major or sickle cell disease.

BACKGROUND: Persistent mixed chimerism represents a state in which recipient and donor cells stably co-exist after hematopoietic stem cell transplantation. However,since in most of the studies reported in literature the engraftment state was observed in the nucleated cells,in this study we determined the donor origin of the mature erythrocytes of patients with persistent mixed chimerism after transplantation for hemoglobinopathies. Results were compared with the engraftment state observed in singly picked out burst-forming unit - erythroid colonies and in the nucleated cells collected from the peripheral blood and from the bone marrow. DESIGN AND METHODS: The donor origin of the erythrocytes was determined analyzing differences on the surface antigens of the erythrocyte suspension after incubation with anti-ABO and/or anti-C,-c,-D,-E and -e monoclonal antibodies by a flow cytometer. Analysis of short tandem repeats was used to determine the donor origin of nucleated cells and burst-forming unit - erythroid colonies singly picked out after 14 days of incubation. RESULTS: The proportions of donor-derived nucleated cells in four transplanted patients affected by hemoglobinopathies were 71%,46%,15% and 25% at day 1364,1385,1314 and 932,respectively. Similar results were obtained for the erythroid precursors,analyzing the donor/recipient origin of the burst-forming unit - erythroid colonies. In contrast,on the same days of observation,the proportions of donor-derived erythrocytes in the four patients with persistent mixed chimerism were 100%,100%,73% and 90%. Conclusions Our results showed that most of the erythrocytes present in four long-term transplanted patients affected by hemoglobinopathies and characterized by the presence of few donor engrafted nucleated cells were of donor origin. The indication that small proportions of donor engrafted cells might be sufficient for clinical control of the disease in patients affected by hemoglobinopathies is relevant,although the biological mechanisms underlying these observations need further investigation. View Publication

产品类型：

产品号#：

04434

04444

84434

84444

产品名：

MethoCult™H4434经典

PancreaCult™类器官生长培养基（小鼠） 建立和维持小鼠胰腺外分泌类器官的细胞培养基

Reference

Yasui K et al. (JAN 2003) Stem cells (Dayton,Ohio) 21 2 143--51

Differences between peripheral blood and cord blood in the kinetics of lineage-restricted hematopoietic cells: implications for delayed platelet recovery following cord blood transplantation.

Cord blood (CB) cells are a useful source of hematopoietic cells for transplantation. The hematopoietic activities of CB cells are different from those of bone marrow and peripheral blood (PB) cells. Platelet recovery is significantly slower after transplantation with CB cells than with cells from other sources. However,the cellular mechanisms underlying these differences have not been elucidated. We compared the surface marker expression profiles of PB and CB hematopoietic cells. We focused on two surface markers of hematopoietic cell immaturity,i.e.,CD34 and AC133. In addition to differences in surface marker expression,the PB and CB cells showed nonidentical differentiation pathways from AC133(+)CD34(+) (immature) hematopoietic cells to terminally differentiated cells. The majority of the AC133(+)CD34(+) PB cells initially lost AC133 expression and eventually became AC133(-)CD34(-) cells. In contrast,the AC133(+)CD34(+) CB cells did not go through the intermediate AC133(-)CD34(+) stage and lost both markers simultaneously. Meanwhile,the vast majority of megakaryocyte progenitors were of the AC133(-)CD34(+) phenotype. We conclude that the delayed recovery of platelets after CB transplantation is due to both subpopulation distribution and the process of differentiation from AC133(+)CD34(+) cells. View Publication

产品类型：

产品号#：

04064

04960

04902

04900

04961

04901

04963

04962

04970

04971

产品名：

入门套件的MethoCult™H4034优化

MegaCult™-C胶原蛋白和不含细胞因子的培养基

胶原蛋白溶液

MegaCult™-C培养基无细胞因子

MegaCult™-C胶原蛋白和细胞因子培养基

MegaCult™-C细胞因子培养基

双室载玻片试剂盒

MegaCult™-C cfu染色试剂盒

MegaCult™-C不含细胞因子完整试剂盒

MegaCult™-C细胞因子完整试剂盒

Reference

Smith MS et al. (SEP 2010) Cell host & microbe 8 3 284--91

Granulocyte-colony stimulating factor reactivates human cytomegalovirus in a latently infected humanized mouse model.

Human cytomegalovirus (HCMV) is a significant cause of morbidity and mortality in organ transplant recipients. The use of granulocyte-colony stimulating factor (G-CSF)-mobilized stem cells from HCMV seropositive donors is suggested to double the risk of late-onset HCMV disease and chronic graft-versus-host disease in recipients when compared to conventional bone marrow transplantation with HCMV seropositive donors,although the etiology of the increased risk is unknown. To understand mechanisms of HCMV transmission in patients receiving G-CSF-mobilized blood products,we generated a NOD-scid IL2Rγ(c)(null)-humanized mouse model in which HCMV establishes latent infection in human hematopoietic cells. In this model,G-CSF induces the reactivation of latent HCMV in monocytes/macrophages that have migrated into organ tissues. In addition to establishing a humanized mouse model for systemic and latent HCMV infection,these results suggest that the use of G-CSF mobilized blood products from seropositive donors pose an elevated risk for HCMV transmission to recipients. View Publication

产品类型：

产品号#：

70008

70008.1

70008.2

70008.3

70008.4

70008.5

70008.6

产品名：

冻存的人脐带血CD34+细胞

人脐带血CD34+细胞，冷冻

抗小鼠CD45R抗体，克隆RA3-6B2 大鼠单克隆IgG2a抗体，抗人、小鼠、猫CD45R（B220）

Technical Bulletin

Fully Automated, High-Purity Cell Isolation for Chimerism Labs

产品类型：

Cell Isolation Products

细胞类型：

B 细胞，T 细胞，淋巴细胞，髓系细胞

产品号#：

21000

20119

20155

17881

17881RF

17884

17884RF

17875

17875RF

17871

17871RF

17874

17874RF

17885

17885RF

17878

17878RF

17876

17876RF

17882

17882RF

17886

17886RF

17888

17888RF

17889

17889RF

17873

17873RF

产品名：

RoboSep™- S

RoboSep™尖端抛光化合物

RoboSep™分选管套装

EasySep™HLA嵌合全血CD15阳性选择试剂盒

EasySep™HLA嵌合全血髓系阳性选择试剂盒

EasySep™HLA嵌合棕褐色大衣CD56阳性选择试剂盒

EasySep™HLA嵌合全血CD3阳性选择试剂盒

EasySep™HLA嵌合全血CD19阳性选择试剂盒

EasySep™HLA嵌合全血CD33阳性选择试剂盒

EasySep™人白细胞抗原嵌合棕毛CD14阳性选择试剂盒

EasySep™人CD33阳性选择试剂盒II

EasySep™HLA嵌合全血CD66b阳性选择试剂盒

EasySep™HLA嵌合全血B细胞阳性选择试剂盒

EasySep™HLA嵌合全血CD4阳性选择试剂盒

EasySep™HLA嵌合全血CD8阳性选择试剂盒

EasySep™HLA嵌合全血淋巴细胞阳性选择试剂盒

Nature Research Round Table: Maintenance of Human Pluripotent Stem Cells In Vitro

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Nature Research Round Table: Maintenance of Human Pluripotent Stem Cells In Vitro

发布日期: 11/1/19

Nature Research Round Table: Genomic Integrity of Human Pluripotent Stem Cells

15:47

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Nature Research Round Table: Genomic Integrity of Human Pluripotent Stem Cells

发布日期: 11/1/19

Human Primary Cells - Get the Cells You Need from STEMCELL Technologies

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Human Primary Cells - Get the Cells You Need from STEMCELL Technologies

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搜索结果: 'methocult media formulations for mouse hematopoietic cells serum containing'

Pharmacologic modulation of the calcium-sensing receptor enhances hematopoietic stem cell lodgment in the adult bone marrow.

Murine stromal cell line HESS-5 maintains reconstituting ability of Ex vivo-generated hematopoietic stem cells from human bone marrow and cytokine-mobilized peripheral blood.

Despite inhibition of hematopoietic progenitor cell growth in vitro, the tyrosine kinase inhibitor imatinib does not impair engraftment of human CD133+ cells into NOD/SCIDbeta2mNull mice.

Quantitatively different red cell/nucleated cell chimerism in patients with long-term, persistent hematopoietic mixed chimerism after bone marrow transplantation for thalassemia major or sickle cell disease.

Differences between peripheral blood and cord blood in the kinetics of lineage-restricted hematopoietic cells: implications for delayed platelet recovery following cord blood transplantation.

Granulocyte-colony stimulating factor reactivates human cytomegalovirus in a latently infected humanized mouse model.

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