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抗小鼠CD45R抗体，克隆RA3-6B2

大鼠单克隆IgG2a抗体，抗人、小鼠、猫CD45R（B220）
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产品号 #60019_C

大鼠单克隆IgG2a抗体，抗人、小鼠、猫CD45R（B220）

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总览
实验数据
产品说明书及文档
应用领域
相关材料与文献
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总览

RA3-6B2抗体识别小鼠CD45外显子A限制性异构体胞外结构域上的一个表位。CD45是蛋白酪氨酸磷酸酶家族的一员，在许多免疫调节过程中起作用，包括细胞活化、生长、分化和致癌转化。CD45的大细胞质部分包含两个酪氨酸磷酸酶结构域（其中一个具有酶活性），参与调节Src激酶（如Lck和Fyn）的功能。CD45的几种亚型已经被鉴定出来，其表达根据细胞类型和功能状态而不同。编码胞外RA， RB和RC多肽序列的三个外显子的选择性剪接产生多达8个分子质量在200 - 220 kDa范围内的异构体。小鼠CD45的外显子A限制性异构体，又称为CD45R，包含RA， RB和RC区域，是最大的异构体。CD45R在B细胞谱系（早期pro-B细胞到成熟B细胞）上表达，在向浆细胞分化的过程中表达下调。CD45R也在T、NK、树突状细胞和淋巴因子激活的杀伤细胞（LAK）亚群上表达。

该抗体克隆已被验证可用于评估EasySep™试剂盒（包括EasySep™小鼠B细胞分选试剂盒（产品号#19854））分选的细胞纯度。

该抗体克隆已被验证可用于评估EasySep™试剂盒（包括EasySep™小鼠B细胞分选试剂盒（产品号#19854））分选的细胞纯度。

该抗体克隆已被验证可用于评估EasySep™试剂盒（包括EasySep™小鼠B细胞分选试剂盒（产品号#19854））分选的细胞纯度。

该抗体克隆已被验证可用于评估EasySep™试剂盒（包括EasySep™小鼠B细胞分选试剂盒（产品号#19854））分选的细胞纯度。

亚型
一抗
 
靶抗原
CD45R (B220)
 
别名
B220，蛋白酪氨酸磷酸酶受体C型，PTPRC
 
活性物种
Cat，人，小鼠
 
偶联
Biotin 或 生物素，FITC，PE，未偶联的
 
宿主物种
大鼠
 
细胞类型
B 细胞，白细胞，淋巴细胞
 
种属
人，小鼠，其它细胞系
 
应用
CyTOF，FACS，流式细胞术，功能学筛选，免疫细胞化学，免疫荧光，免疫组化，免疫沉淀，Western印迹
 
研究领域
免疫，移植研究
 
克隆
RA3-6B2
 
基因编号
19264/5788
 
同种型
IgG2a，kappa
 

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实验数据

Data for Unconjugated

Figure 1. Data for Unconjugated

Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD45R Antibody, Clone RA3-6B2, followed by a mouse anti-rat IgG2a antibody, FITC (filled histogram), or Rat IgG2a, kappa Isotype Control Antibody, Clone RTK2758 (Catalog #60076), followed by a mouse anti-rat IgG2a antibody, FITC (solid line histogram).

Data for PE-Conjugated

Figure 2. Data for PE-Conjugated

Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD45R Antibody, Clone RA3-6B2, PE (filled histogram) or Rat IgG2a, kappa Isotype Control Antibody, Clone RTK2758, PE (Catalog #60076PE) (solid line histogram).

Data for APC-Conjugated

Figure 3. Data for APC-Conjugated

Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD45R Antibody, Clone RA3-6B2, APC (filled histogram) or Rat IgG2a, kappa Isotype Control Antibody, Clone RTK2758, APC (Catalog #60076AZ) (solid line histogram).

Data for FITC-Conjugated

Figure 4. Data for FITC-Conjugated

Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD45R Antibody, Clone RA3-6B2, FITC (filled histogram) or Rat IgG2a, kappa Isotype Control Antibody, Clone RTK2758, FITC (Catalog #60076FI) (solid line histogram).

Data for PerCP-Cy55-Conjugated

Figure 5. Data for PerCP-Cy55-Conjugated

Flow cytometry analysis fo C57BL/6 mouse splenocytes labeled with Anti-Mouse CD45R Antibody, Clone RA3-6B2, PerCP-Cy5.5 (filled histogram) or a rat IgG2a, kappa isotype control antibody, PerCP-Cy5.5 (solid line histogram).

Data for Alexa Fluor® 488-Conjugated

Figure 6. Data for Alexa Fluor® 488-Conjugated

Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD45R Antibody, Clone RA3-6B2, Alexa Fluor® 488 (filled histogram) or Rat IgG2a, kappa Isotype Control Antibody, Clone RTK2758, Alexa Fluor® 488 (Catalog #60076AD) (solid line histogram).

Data for Biotin-Conjugated

Figure 7. Data for Biotin-Conjugated

Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD45R Antibody, Clone RA3-6B2, Biotin, followed by streptavidin (SAV) APC (filled histogram), or Rat IgG2a, kappa Isotype Control Antibody, Clone RTK2758, Biotin (Catalog #60076BT), followed by SAV APC (solid line histogram).

Figure 8. Data for PB-Conjugated

Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD45R Antibody, Clone RA3-6B2, Pacific Blue™ (filled histogram) or a rat IgG2a, kappa Pacific Blue™ isotype control antibody (solid line histogram).

产品说明书及文档

请在《产品说明书》中查找相关支持信息和使用说明，或浏览下方更多实验方案。

Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet
Product Name
Anti-Mouse CD45R Antibody, Clone RA3-6B2, Alexa Fluor® 488
Catalog #
60019AD, 60019AD.1
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Mouse CD45R Antibody, Clone RA3-6B2, APC
Catalog #
60019AZ, 60019AZ.1
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Mouse CD45R Antibody, Clone RA3-6B2, Biotin
Catalog #
100-0425, 100-0424, 60019BT
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Mouse CD45R Antibody, Clone RA3-6B2, FITC
Catalog #
100-0423, 60019FI
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Mouse CD45R Antibody, Clone RA3-6B2, Pacific Blue™
Catalog #
60019PB, 60019PB.1
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Mouse CD45R Antibody, Clone RA3-6B2, PE
Catalog #
100-0421, 100-0420
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Mouse CD45R Antibody, Clone RA3-6B2, PerCP-Cy5.5
Catalog #
60019PS, 60019PS.1
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Mouse CD45R Antibody, Clone RA3-6B2
Catalog #
100-0422, 60019
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Mouse CD45R Antibody, Clone RA3-6B2, Alexa Fluor® 488
Catalog #
60019AD, 60019AD.1
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Mouse CD45R Antibody, Clone RA3-6B2, APC
Catalog #
60019AZ, 60019AZ.1
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Mouse CD45R Antibody, Clone RA3-6B2, Biotin
Catalog #
100-0425, 100-0424, 60019BT
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Mouse CD45R Antibody, Clone RA3-6B2, FITC
Catalog #
100-0423, 60019FI
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Mouse CD45R Antibody, Clone RA3-6B2, Pacific Blue™
Catalog #
60019PB, 60019PB.1
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Mouse CD45R Antibody, Clone RA3-6B2, PE
Catalog #
100-0421, 100-0420
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Mouse CD45R Antibody, Clone RA3-6B2, PerCP-Cy5.5
Catalog #
60019PS, 60019PS.1
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Mouse CD45R Antibody, Clone RA3-6B2
Catalog #
100-0422, 60019
Lot #
All
Language
English

应用领域

本产品专为以下研究领域设计，适用于工作流程中的高亮阶段。探索这些工作流程，了解更多我们为各研究领域提供的其他配套产品。

Research Area
Workflow Stages

更多信息

更多信息
种属 Human, Mouse, Other
克隆 RA3-6B2
Gene Id 19264/5788
Alternative Names B220, Protein tyrosine phosphatase receptor type C, PTPRC
同种型/isotype IgG2a, kappa
法律声明:

Alexa Fluor and Pacific Blue are trademarks of Life Technologies Corporation. Antibodies conjugated to Alexa Fluor® or Pacific Blue™ are licensed for internal research use only and sale is expressly conditioned on the buyer not using the antibody for manufacturing, performing a service or medical test, or otherwise generating revenue. For use other than research, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com. 质量保证:

产品仅供研究使用，不用于针对人或动物的诊断或治疗。 欲获悉更多关于STEMCELL的质控信息，请访问 STEMCELL.CN/COMPLIANCE.
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