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  • 搜索结果: 'methocult media formulations for human hematopoietic cells serum containing'

搜索结果: 'methocult media formulations for human hematopoietic cells serum containing'

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  3. Preclinical ex vivo expansion of cord blood hematopoietic stem and progenitor cells: duration of culture; the media, serum supplements, and growth factors used; and engraftment in NOD/SCID mice.
    Reference
    Lam AC et al. (DEC 2001) Transfusion 41 12 1567--76

    Preclinical ex vivo expansion of cord blood hematopoietic stem and progenitor cells: duration of culture; the media, serum supplements, and growth factors used; and engraftment in NOD/SCID mice.

    BACKGROUND: Ex vivo expansion of cord blood (CB) hematopoietic stem and progenitor cells increases cell dose and may reduce the severity and duration of neutropenia and thrombocytopenia after transplantation. This study's purpose was to establish a clinically applicable culture system by investigating the use of cytokines,serum-free media,and autologous plasma for the expansion of CB cells and the engraftment of expanded product in nonobese diabetic/severe combined immunodeficient (NOD/SCID) mice. STUDY DESIGN AND METHODS: Enriched CB CD34+ cells were cultured in four media (Iscove's modified Dulbecco's medium with FCS,Gibco; X-Vivo-10,BioWhittaker; QBSF-60,Quality Biological; and StemSpan SFEM,Stem Cell Technologies) with four cytokine combinations (thrombopoietin [TPO],SCF,Flt-3 ligand [FL] with and without G-CSF,and/or IL-6). The effect of autologous CB plasma was also investigated. The read-out measures were evaluated on Days 8 and 12. After expansion at the optimized condition,cultured cells were transplanted into sublethally irradiated NOD/SCID mice. The engraftment of human CD45+ cells and subsets in the bone marrow,spleen,and peripheral blood was determined. RESULTS: QBSF-60 or StemSpan SFEM supported high yields of early progenitors (CD34+ cells,textlessor= 64.8-fold; CD34+CD38- cells,330-fold; CFU-granulocyte erythroid macrophage megakaryocyte [GEMM],248-fold) and CFUs of the myeloid (CFU-GM,407-fold) and erythroid (BFU/CFU-E,144-fold) lineages. The expansion of the megakaryocytic lineage was consistently higher in X-Vivo-10 (CFU-megakaryocyte,684-fold). Autologous plasma promoted colony formation but reduced CD34+ cells and CFU-GEMM. The addition of G-CSF or IL-6 improved cell yields; G-CSF was more effective for committed progenitors. Expansion products from cultures in QBSF-60 with the cytokines engrafted and differentiated into the myeloid and lymphoid lineages in NOD/SCID mice. CONCLUSION: The data supported the strategy of expansion. The optimized condition may be applicable to clinical expansion for the abrogation or reduction of posttransplant cytopenia. View Publication

    产品类型：

    产品号#：

    09600

    09650

    产品名：

    StemSpan™SFEM

    StemSpan™SFEM

  10. Human Colony-Forming Unit (CFU) Assays Using MethoCult™
    Technical Manual
    Human Colony-Forming Unit (CFU) Assays Using MethoCult™

    产品类型：

    Cell Culture Media and Supplements

    产品号#：

    00217

    00217UK

    00215

    00215GER

    00215UK

    00602

    00603

    00606

    00607

    00608

    00609

    04436

    04064

    04100

    04230

    04236

    04431

    04434

    04444

    04464

    04531

    04535

    04545

    04536

    04564

    09300

    04035

    04330

    04034

    04044

    04435

    04445

    04534

    04544

    04437

    04447

    00215US.4

    00215CA.3

    00215GER.3

    0

    产品名：

    MethoCult™SF H4436

    入门套件的MethoCult™H4034优化

    MethoCult™H4100

    MethoCult™H4230

    MethoCult™SF H4236

    MethoCult™H4431

    MethoCult™H4434经典

    MethoCult™H4434经典

    入门套件为MethoCult™H4434经典

    MethoCult™H4531

    MethoCult™H4535富集无EPO

    MethoCult™ H4535 Enriched，不含EPO

    MethoCult™SF H4536

    入门套件MethoCult™H4534经典无EPO

    含有10% 牛血清白蛋白（BSA）的 Iscove's MDM

    MethoCult™H4035 Optimum无EPO

    MethoCult™H4330

    MethoCult™H4034 Optimum

    MethoCult™H4034 Optimum

    MethoCult™H4435富集

    MethoCult™H4435富集

    MethoCult™H4534经典无EPO

    MethoCult™H4534经典无EPO

    MethoCult™表达

    MethoCult™表达

    Standardization of the Hematopoietic Progenitor Assay Training Course

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