搜索结果: 'ipsc'

PBS-MINI MagDrive生物反应器 紧凑型生物反应器，配备一次性容器，用于hPSC和其他细胞类型的高通量3D悬浮培养
eTeSR™ 稳定的、无饲养层的培养基，适用于人类多能干细胞的单细胞传代
mFreSR™ 用于人胚胎干细胞和诱导多能干细胞的无血清冻存液
新品
STEMmatrix™ BME
基底膜提取物，在无饲养层的人多能干细胞 (hPSCs) 培养体系中有效支持细胞维持于未分化状态。
温和细胞解离试剂 cGMP，无酶细胞解离试剂
STEMdiff™ 心肌细胞铺板添加物
人多能干细胞衍生的心肌细胞在复苏及收集后提高铺板效率的专用添加物
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Start with High-Quality Human iPSC Solutions

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TeSR

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Jung-Klawitter S et al. (OCT 2016) Stem cell research 17 3 580--583

Generation of an iPSC line from a patient with tyrosine hydroxylase (TH) deficiency: TH-1 iPSC.

Fibroblasts from a male patient with compound heterozygous variants in the tyrosine hydroxylase gene (TH; OMIM: 191290; c.[385-CtextgreaterT]; [692-GtextgreaterC]/p.[R129*]; [R231P]),the rate-limiting enzyme for dopamine synthesis,were reprogrammed to iPSCs using episomal reprogramming delivering the reprogramming factors Oct3/4,Sox2,L-Myc,Lin28,Klf4 and p53 shRNA Okita et al. (2011). Pluripotency of TH-1 iPSC was verified by immunohistochemistry and RT-PCR analysis. Cells exhibited a normal karyotype and differentiated spontaneously into the 3 germ layers in vitro. TH-1 iPSC represents the first model system to study the pathomechanism of this rare metabolic disease and provides a useful tool for drug testing. View Publication

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85850

85857

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mTeSR™1

mTeSR™1
19:53

线上讲座
GMP-Grade Human iPSC Lines and Matched Research-Grade Lines
Stockmann M et al. (AUG 2013) Stem Cell Reviews and Reports 9 4 475--492

Developmental and Functional Nature of Human iPSC Derived Motoneurons

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07913

85850

85857

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Dispase（5 U/mL）

mTeSR™1

mTeSR™1
Chen G et al. (MAY 2011) Nature methods 8 5 424--9

Chemically defined conditions for human iPSC derivation and culture.

We re-examine the individual components for human embryonic stem cell (ESC) and induced pluripotent stem cell (iPSC) culture and formulate a cell culture system in which all protein reagents for liquid media,attachment surfaces and splitting are chemically defined. A major improvement is the lack of a serum albumin component,as variations in either animal- or human-sourced albumin batches have previously plagued human ESC and iPSC culture with inconsistencies. Using this new medium (E8) and vitronectin-coated surfaces,we demonstrate improved derivation efficiencies of vector-free human iPSCs with an episomal approach. This simplified E8 medium should facilitate both the research use and clinical applications of human ESCs and iPSCs and their derivatives,and should be applicable to other reprogramming methods. View Publication

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07180

07183

100-0763

72482

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Vitronectin XF™

CellAdhere™ 稀释缓冲液

Vitronectin XF™

HA-100 (Dihydrochloride)
Mekhoubad S et al. (MAY 2012) Cell stem cell 10 5 595--609

Erosion of dosage compensation impacts human iPSC disease modeling.

Although distinct human induced pluripotent stem cell (hiPSC) lines can display considerable epigenetic variation,it has been unclear whether such variability impacts their utility for disease modeling. Here,we show that although low-passage female hiPSCs retain the inactive X chromosome of the somatic cell they are derived from,over time in culture they undergo an erosion" of X chromosome inactivation (XCI). This erosion of XCI is characterized by loss of XIST expression and foci of H3-K27-trimethylation� View Publication

产品类型：

产品号#：

85850

85857

产品名：

mTeSR™1

mTeSR™1