搜索结果: 'easysep direct'

科学海报
Fast and Easy Isolation of CD27-Positive Human Memory B Cells Using EasySep™ Releasable RapidSpheres™

产品类型：

Conference：

AAI 2017

产品号#：

17864

产品名：

EasySep™ 人记忆B细胞分选试剂盒
36:41

视频
How to Isolate Human Regulatory T Cells (Tregs) Using the Easy 50 EasySep™ Magnet

发布日期: 05/07/2021
14:21

视频
How to Isolate Immune Cells from Large-Volume Samples Using the Easy 250 EasySep™ Magnet

发布日期: 04/20/2022
技术公告
Achieve Scalable, High-Quality Nucleic Acid Extractions with the EasySep™ Total Nucleic Acid Extraction Kit

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细胞类型：

B细胞，NK细胞，T细胞，其他细胞系，单个核细胞，单核细胞，巨噬细胞，树突状细胞（DCs），淋巴细胞，癌细胞及细胞系，粒细胞及其亚群，肿瘤细胞

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发布日期: 09/11/2025
科学海报
Optimized Capture and Purification of Nucleic Acid Using the EasySep™ Total Nucleic Acid Extraction Kit, A Robust Magnetic Particle-Based Extraction System

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科学海报
Immunomagnetic Cell Isolation of Untouched Lymphocyte Populations Directly from Whole Blood

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Conference：

AAI 2014

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19655

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18000

19659

19655RF

89671

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产品名：

EasySep™ Direct人总淋巴细胞分选试剂盒

EasySep™ Direct人T细胞分选试剂盒

EasySep™ Direct人CD4+ T细胞分选试剂盒

EasySep™ Direct人CD8+ T细胞分选试剂盒

EasySep™ Direct 人B-CLL细胞分选试剂盒

EasySep™ Direct人B细胞分选试剂盒

EasySep™磁极

EasySep™ Direct人Pan-粒细胞分选试剂盒

RoboSep™ Direct人总淋巴细胞分选试剂盒

EasySep™ Direct HLA交叉配型T细胞分选试剂盒

RoboSep™ Direct HLA交叉配型T细胞分选试剂盒

EasySep™ Direct HLA交叉配型B细胞分选试剂盒

RoboSep™ Direct HLA交叉配型B细胞分选试剂盒
R. J. Komban et al. ( 2019) Nature communications 10 1 2423

Activated Peyer's patch B cells sample antigen directly from M cells in the subepithelial dome.

The germinal center (GC) reaction in Peyer's patches (PP) requires continuous access to antigens,but how this is achieved is not known. Here we show that activated antigen-specific CCR6+CCR1+GL7- B cells make close contact with M cells in the subepithelial dome (SED). Using in situ photoactivation analysis of antigen-specific SED B cells,we find migration of cells towards the GC. Following antigen injection into ligated intestinal loops containing PPs,40{\%} of antigen-specific SED B cells bind antigen within 2 h,whereas unspecifc cells do not,indicating B cell-receptor involvment. Antigen-loading is not observed in M cell-deficient mice,but is unperturbed in mice depleted of classical dendritic cells (DC). Thus,we report a M cell-B cell antigen-specific transporting pathway in PP that is independent of DC. We propose that this antigen transporting pathway has a critical role in gut IgA responses,and should be taken into account when developing mucosal vaccines. View Publication

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产品号#：

19854

19854RF

产品名：

EasySep™小鼠B细胞分选试剂盒

RoboSep™ 小鼠B细胞分选试剂盒
科学海报
Rapid, one-step isolation of granulocytes directly from whole blood without RBC lysis, sedimentation or density spearation

产品类型：

Conference：

KEYSTONE 2016

产品号#：

19659

产品名：

EasySep™ Direct人Pan-粒细胞分选试剂盒
Staton PJ et al. (APR 2006) Journal of immunology (Baltimore,Md. : 1950) 176 7 3978--86

IL-7 is a critical factor in modulating lesion development in Skn-directed autoimmunity.

In a murine model of autoimmunity targeted against the epidermal cell Ags,Skn,adoptive transfer of Skn-immune T cells to immunosuppressed recipients elicits skin lesions in areas of mild epidermal trauma. In this study,we examined peripheral regulation of Skn-induced autoreactivity disrupted by rendering the mice immunoincompetent. We found that regulation of Skn-directed autoimmunity was restored by cotransfer of normal syngeneic spleen cells at twice the concentration of Skn-immune cells and was evidenced by significantly reduced lesion severity by days 5-7 post-cotransfer compared with animals given injections of Skn-immune cells alone. Enrichment and depletion of normal CD4(+) or CD8(+) spleen cells and RT-PCR analysis of selected cytokines identified CD4(+) cells as the regulatory cells in the cotransfer inoculum; however,significant reduction in lesion severity was observed only when there was a concomitant increase in levels of IL-7. The role of IL-7 was further supported in that mice cotransferred with Skn-immune cells plus normal spleen cells,but also treated with anti-IL-7 Ab,no longer exhibited reduced lesion severity. To determine whether IL-7 expression without normal spleen cell cotransfer could modulate lesion development,an IL-7-encoding plasmid (pCMV-Tag1-IL-7) was topically delivered to sites flanking the stressed skin site in Skn-induced autoimmune mice. Daily application of 15 mug of pCMV-Tag1-IL-7 significantly suppressed lesion severity. Our results support a mechanism for CD4(+) T cells and IL-7 in contributing to the control of autoreactivity. View Publication

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Li Y et al. (FEB 2016) Journal of Immunology 196 4 1617--25

Hepatic Stellate Cells Directly Inhibit B Cells via Programmed Death-Ligand 1.

We demonstrated previously that mouse hepatic stellate cells (HSCs) suppress T cells via programmed death-ligand 1 (PD-L1),but it remains unknown whether they exert any effects on B cells,the other component of the adaptive immune system. In this study,we found that mouse HSCs directly inhibited B cells and that PD-L1 was also integrally involved. We found that HSCs inhibited the upregulation of activation markers on activated B cells,as well as the proliferation of activated B cells and their cytokine/Ig production in vitro,and that pharmaceutically or genetically blocking the interaction of PD-L1 with programmed cell death protein 1 impaired the ability of HSCs to inhibit B cells. To test the newly discovered B cell-inhibitory activity of HSCs in vivo,we developed a protocol of intrasplenic artery injection to directly deliver HSCs into the spleen. We found that local delivery of wild-type HSCs into the spleens of mice that had been immunized with 4-hydroxy-3-nitrophenylacetyl-Ficoll,a T cell-independent Ag,significantly suppressed Ag-specific IgM and IgG production in vivo,whereas splenic artery delivery of PD-L1-deficient HSCs failed to do so. In conclusion,in addition to inhibiting T cells,mouse HSCs concurrently inhibit B cells via PD-L1. This direct B cell-inhibitory activity of HSCs should contribute to the mechanism by which HSCs maintain the liver's immune homeostasis. View Publication

产品类型：

产品号#：

19854

19854RF

产品名：

EasySep™小鼠B细胞分选试剂盒

RoboSep™ 小鼠B细胞分选试剂盒
技术窍门
优化EasySep™ Releasable RapidSpheres™间接正选试剂盒的使用
产品手册
EasySep™细胞分选技术 - 更高效地分离细胞

产品类型：

品牌：

EasySep

产品号#：

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产品名：

EasySep™人CD4+CD127low CD25+调节性T细胞分选试剂盒

EasySep™人CD3正选试剂盒II

RoboSep™ 人CD3正选试剂盒II

EasySep™人CD4正选试剂盒II

RoboSep™ 人CD4正选试剂盒II

EasySep™人CD8正选试剂盒 II

RoboSep™ 人CD8正选试剂盒 II

EasySep™人CD19正选试剂盒II

RoboSep™ 人CD19正选试剂盒II

EasySep™人CD56正选试剂盒 II

RoboSep™ 人CD56正选试剂盒 II

EasySep™人CD14正选试剂盒II

RoboSep™ 人CD14正选试剂盒II

EasySep™ 人脐带血CD34正选试剂盒 II

RoboSep™ 人脐带血CD34正选试剂盒II

EasySep™人T细胞分选试剂盒

RoboSep™ 人T细胞分选试剂盒

EasySep™人CD4+ T细胞分选试剂盒

RoboSep™ 人CD4+ T细胞分选试剂盒

EasySep™人CD8+ T细胞分选试剂盒

RoboSep™ 人CD8+ T细胞分选试剂盒