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Isolate Multiple Cell Types from a Single Sample with Sequential Isolation Sequential separation, for higher recovery of purified cells from limited sample size and isolation of multiple cell types, is ideal for chimerism analysis
Using EasySep™ Positive Selection Kits for Cell Depletion The EasySep™ system allows you to use a positive selection kit to deplete the labeled cell type, by making changes to the separation scheme of the protocol
DNase I Treatment for Clumpy Cell Suspensions To reduce cell clumping and minimize presence of free-floating DNA fragments, we recommend adding DNase I Solution (Catalog #07900) to the cell suspension
Total Nucleated Cell and Viable Cell Counts Accurate cell counts are important in experiments. Ideally both the total nucleated cell (TNC) count and the viability of the cells should be assessed
Isolating Cells from Atypical Samples Technical tip from our dedicated team of Product and Scientific Support specialists
Performing Total Nucleated Cell Counts with a Hemocytometer Technical tip from our dedicated team of Product and Scientific Support specialists
Preparing a Polymorphonuclear Cell Fraction from Whole Blood using Ammonium Chloride Lysis Technical tip from our dedicated team of Product and Scientific Support specialists
Improving Cell Recovery with EasySep™ Negative Selection Kits Technical tip from our dedicated team of Product and Scientific Support specialists
Using Cell Density Gradients to Optimize Your Cell Isolations RosetteSep™, a unique immunodensity cell isolation platform, isolates highly purified cells by density gradient centrifugation without columns or magnets
Isolate Virtually Any Cell Type Using EasySep™ or EasySep™ Release Indirect Selection Kits Use any biotinylated, FITC-, PE- or APC-conjugated antibody to label your desired cells and mix with EasySep™ reagents to obtain highly purified cells
Regulatory T Cells Overview of the development, phenotype and functions of regulatory T cells
Working with Small Sample Volumes or Low Start Cell Numbers Using the Purple EasySep™ and EasyPlate™ EasySep™ Platforms Technical tip from our dedicated team of Product and Scientific Support specialists