Optimized Workflows for High-Efficiency Genome Editing in Stem and Primary Cell Types
CRISPR-Cas genome editing in cell culture systems is a powerful technique for disease modeling and the development of cellular therapies. Compared to work with immortalized cell lines, genome editing of stem and primary cells presents unique challenges, including issues related to efficient delivery and expression of CRISPR machinery, clonogenicity, and cytotoxicity. In this webinar, our in-house expert, Dr. Ashley Watson, discusses optimized workflows for CRISPR-Cas9 genome editing in human pluripotent stem cells and primary T cells.
Nature Research Round Table: Retinal Cell Therapy Using Human Embryonic Stem Cells
Dr. Peter Coffey from University College London shares his work with the London Project to Cure Blindness, which resulted in the first clinical stem cell trial to treat blindness, and discusses cell quality requirements for clinical trials. This presentation and the following Q&A session were moderated by Dr. Christine Mummery from the Leiden University Medical Center.
This presentation was part of a Round Table series titled “Challenges in Ensuring hPSC Quality”, hosted in partnership with Nature Research. Global experts gathered at the Springer Nature headquarters in London, UK, to tackle some of the most pertinent issues impacting the use of human pluripotent stem cells (hPSCs), ranging from fundamental biology research to therapeutic applications. Explore the full series here.
Note: Some original data from this presentation has been omitted to abide by copyright rules.
Nature Research Round Table: Parkinson's Disease Therapy with Human Embryonic Stem Cells
Dr. Malin Parmar from Lund University talks about her work on cell-based therapies to treat Parkinson’s disease, as well as regulatory, safety, and efficacy requirements for the clinic. This presentation and the following Q&A session were moderated by Dr. Christine Mummery from the Leiden University Medical Center.
This presentation was part of a Round Table series titled “Challenges in Ensuring hPSC Quality”, hosted in partnership with Nature Research. Global experts gathered at the Springer Nature headquarters in London, UK, to tackle some of the most pertinent issues impacting the use of human pluripotent stem cells (hPSCs), ranging from fundamental biology research to therapeutic applications. Explore the full series here.
Note: Some original data from this presentation has been omitted to abide by copyright rules.
How to Use Primary Cells to Develop the Next Therapy
Human primary cells are cells isolated directly from tissues, including blood and bone marrow. The use of human primary cells increases the physiological relevance of cell culture systems, enabling you to generate meaningful data that is more predictive of in vivo outcomes. Learn about the advantages of starting with human primary cells for your immunology and cell therapy research workflows. Key considerations for choosing the ideal starting material, donor characterization, regulatory challenges, and tips to streamline your cell-based assays with human primary and cultured cells are also covered.
“Laser-Based Cell Manipulation” Featuring Dr. Marinna Madrid
On this episode of the Stem Cell Podcast, Dr. Marinna Madrid from Cellino discusses how lasers and image-guided machine learning can make iPSC-derived cell therapies viable at a large scale.
EasySep™小鼠TIL（CD45）正选试剂盒
Maturation of Culture-Expanded Erythroid Progenitor Cells Technical tip from our dedicated team of Product and Scientific Support specialists
Optimized Workflows for High-Efficiency Genome Editing in Stem and Primary Cell Types CRISPR-Cas genome editing in cell culture systems is a powerful technique for disease modeling and the development of cellular therapies. Compared to work with immortalized cell lines, genome editing of stem and primary cells presents unique challenges, including issues related to efficient delivery and expression of CRISPR machinery, clonogenicity, and cytotoxicity. In this webinar, our in-house expert, Dr. Ashley Watson, discusses optimized workflows for CRISPR-Cas9 genome editing in human pluripotent stem cells and primary T cells.
Nature Research Round Table: Retinal Cell Therapy Using Human Embryonic Stem Cells Dr. Peter Coffey from University College London shares his work with the London Project to Cure Blindness, which resulted in the first clinical stem cell trial to treat blindness, and discusses cell quality requirements for clinical trials. This presentation and the following Q&A session were moderated by Dr. Christine Mummery from the Leiden University Medical Center.
Nature Research Round Table: Parkinson's Disease Therapy with Human Embryonic Stem Cells Dr. Malin Parmar from Lund University talks about her work on cell-based therapies to treat Parkinson’s disease, as well as regulatory, safety, and efficacy requirements for the clinic. This presentation and the following Q&A session were moderated by Dr. Christine Mummery from the Leiden University Medical Center.
How to Use Primary Cells to Develop the Next Therapy Human primary cells are cells isolated directly from tissues, including blood and bone marrow. The use of human primary cells increases the physiological relevance of cell culture systems, enabling you to generate meaningful data that is more predictive of in vivo outcomes. Learn about the advantages of starting with human primary cells for your immunology and cell therapy research workflows. Key considerations for choosing the ideal starting material, donor characterization, regulatory challenges, and tips to streamline your cell-based assays with human primary and cultured cells are also covered.
CAR T Cell Manufacturing Workflow: Isolation, Activation and Expansion Learn about human T cell isolation, activation, and expansion workflow for cell therapy
How to Isolate Cells with EasySep™ Column-Free Cell Separation Technology Isolation of human CD4+ T cells by negative selection using EasySep™ to separate highly purified cells in as little as 8 minutes
“Laser-Based Cell Manipulation” Featuring Dr. Marinna Madrid On this episode of the Stem Cell Podcast, Dr. Marinna Madrid from Cellino discusses how lasers and image-guided machine learning can make iPSC-derived cell therapies viable at a large scale.
