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Tools for Optimizing Human Immune Cell Research Obtaining consistent and reliable results when culturing immune cells can be challenging. Watch this webinar to discover how to obtain high yields of functional T cells, NK cells, B cells, dendritic cells, and macrophages for your research applications. The speaker, Evan Karas, also explains how to expand primary T cells without feeders or serum.

How to Co-Culture Human Airway Epithelial and Immune Cells for RSV Infection

How to Deplete Red Blood Cells from Small Volume Samples with HetaSep™ How to use an erythrocyte aggregation agent to remove red blood cells (RBCs) from small volume blood samples prior to colony-forming unit (CFU) assays

Importance of Using an FcR Blocker During Cell Separation Procedures EasySep™ Other Species indirect selection kits can be used to isolate cells from any species

Isolate Multiple Cell Types from a Single Sample with Sequential Isolation Sequential separation, for higher recovery of purified cells from limited sample size and isolation of multiple cell types, is ideal for chimerism analysis

DNase I Treatment for Clumpy Cell Suspensions To reduce cell clumping and minimize presence of free-floating DNA fragments, we recommend adding DNase I Solution (Catalog #07900) to the cell suspension

Using Cell Density Gradients to Optimize Your Cell Isolations RosetteSep™, a unique immunodensity cell isolation platform, isolates highly purified cells by density gradient centrifugation without columns or magnets

Total Nucleated Cell and Viable Cell Counts Accurate cell counts are important in experiments. Ideally both the total nucleated cell (TNC) count and the viability of the cells should be assessed

Using EasySep™ Positive Selection Kits for Cell Depletion The EasySep™ system allows you to use a positive selection kit to deplete the labeled cell type, by making changes to the separation scheme of the protocol

Performing Total Nucleated Cell Counts with a Hemocytometer Technical tip from our dedicated team of Product and Scientific Support specialists

Preparing a Polymorphonuclear Cell Fraction from Whole Blood using Ammonium Chloride Lysis Technical tip from our dedicated team of Product and Scientific Support specialists

How to Isolate Cells with EasySep™ Column-Free Cell Separation Technology Isolation of human CD4+ T cells by negative selection using EasySep™ to separate highly purified cells in as little as 8 minutes