切换导航
  • 比较产品
我的购物车

若您需要咨询产品或有任何技术问题，请通过官方电话 400 885 9050 或邮箱 info.cn@stemcell.com 与我们联系

EasySep™ HLA B细胞富集： 用于处理全血的全套试剂盒

从经HetaSep™处理的全血中通过免疫磁珠负选分选不带标记的人B细胞
率先评论此产品
添加并比较

产品号 #（选择产品）

产品号 #19954HLA_C

从经HetaSep™处理的全血中通过免疫磁珠负选分选不带标记的人B细胞

产品优势

  • 操作简单、快捷，且无需分离柱
  • 纯度高达99%
  • 获得不带标记的活细胞
  • 与下游HLA检测兼容

产品组分包括

  • EasySep™ HLA B细胞富集： 用于处理全血的全套试剂盒（产品号 #19954HLA）
    • EasySep™HLA B细胞富集抗体混合物，1 mL
    • EasySep™ D Magnetic Particles磁珠，4 x 1 mL
    • HetaSep™，20 mL（产品号 #07806）× 2
  • RoboSep™ HLA B细胞富集：用于处理全血的全套试剂盒（含过滤吸头）（产品号 #19954HLARF）
    • EasySep™HLA B细胞富集抗体混合物，1 mL
    • EasySep™ D Magnetic Particles磁珠，4 x 1 mL
    • HetaSep™，20 mL（产品号 #07806）× 2
    • RoboSep™ 缓冲液（产品号 #20104）
    • RoboSep™过滤吸头（产品号 #20125）x 2
main product photo
New look, same high quality and support! You may notice that your instrument or reagent packaging looks slightly different from images displayed on the website, or from previous orders. We are updating our look but rest assured, the products themselves and how you should use them have not changed. Learn more
专为您的实验方案打造的产品
要查看实验方案所需的所有配套产品，请参阅《实验方案与技术文档》。
  1. EasySep™ Magnet
    EasySep™ Magnet

    Magnet for column-free immunomagnetic separation

  2. EasySep™ Buffer
    EasySep™ Buffer

    Cell separation buffer

总览
实验数据
产品说明书及文档
应用领域
相关材料与文献
相关产品

总览

是用EasySep™ HLA B细胞富集： 用于处理全血的全套试剂盒，可简便高效地从经HetaSep™处理的全血或洗涤后的白细胞单采术样本中，通过免疫磁珠负选法分离高纯度的人B细胞。EasySep™技术结合单克隆抗体的特异性和无柱磁分选系统的简便性，已在发表的研究中广泛应用超过20年。

在该EasySep™负选流程中，非目的细胞通过抗体复合物与磁珠标记。表达以下标志物的非目的细胞被靶向去除：CD2、CD3、CD14、CD16、CD33、CD36、CD41、CD43、CD56、CD66b及血型糖蛋白A（glyA）。使用EasySep™磁极吸附后，通过简单地将目的B细胞倾倒或吸取至一个新的试管中，即可将被磁珠标记的细胞与不带标记的目的细胞分离开来。经磁珠分选后，目的B细胞可立即用于流式细胞术、细胞培养或DNA/RNA提取等下游应用。

了解更多EasySep™免疫磁珠技术的工作原理，或者如何通过RoboSep™实现全自动化免疫磁珠细胞分选。探索更多为您的实验流程优化的产品，包括细胞鉴定、冻存等。

在该EasySep™负选流程中，非目的细胞通过抗体复合物与磁珠标记。表达以下标志物的非目的细胞被靶向去除：CD2、CD3、CD14、CD16、CD33、CD36、CD41、CD43、CD56、CD66b及血型糖蛋白A（glyA）。使用EasySep™磁极吸附后，通过简单地将目的B细胞倾倒或吸取至一个新的试管中，即可将被磁珠标记的细胞与不带标记的目的细胞分离开来。经磁珠分选后，目的B细胞可立即用于流式细胞术、细胞培养或DNA/RNA提取等下游应用。

了解更多EasySep™免疫磁珠技术的工作原理，或者如何通过RoboSep™实现全自动化免疫磁珠细胞分选。探索更多为您的实验流程优化的产品，包括细胞鉴定、冻存等。

在该EasySep™负选流程中，非目的细胞通过抗体复合物与磁珠标记。表达以下标志物的非目的细胞被靶向去除：CD2、CD3、CD14、CD16、CD33、CD36、CD41、CD43、CD56、CD66b及血型糖蛋白A（glyA）。使用EasySep™磁极吸附后，通过简单地将目的B细胞倾倒或吸取至一个新的试管中，即可将被磁珠标记的细胞与不带标记的目的细胞分离开来。经磁珠分选后，目的B细胞可立即用于流式细胞术、细胞培养或DNA/RNA提取等下游应用。

了解更多EasySep™免疫磁珠技术的工作原理，或者如何通过RoboSep™实现全自动化免疫磁珠细胞分选。探索更多为您的实验流程优化的产品，包括细胞鉴定、冻存等。

在该EasySep™负选流程中，非目的细胞通过抗体复合物与磁珠标记。表达以下标志物的非目的细胞被靶向去除：CD2、CD3、CD14、CD16、CD33、CD36、CD41、CD43、CD56、CD66b及血型糖蛋白A（glyA）。使用EasySep™磁极吸附后，通过简单地将目的B细胞倾倒或吸取至一个新的试管中，即可将被磁珠标记的细胞与不带标记的目的细胞分离开来。经磁珠分选后，目的B细胞可立即用于流式细胞术、细胞培养或DNA/RNA提取等下游应用。

了解更多EasySep™免疫磁珠技术的工作原理，或者如何通过RoboSep™实现全自动化免疫磁珠细胞分选。探索更多为您的实验流程优化的产品，包括细胞鉴定、冻存等。

磁体兼容性
• EasySep™ Magnet (Catalog #18000) • “The Big Easy” EasySep™ Magnet (Catalog #18001) • Easy 50 EasySep™ Magnet (Catalog #18002) • EasyEights™ EasySep™ Magnet (Catalog #18103) • RoboSep™-S (Catalog #21000)
 
亚型
细胞分选试剂盒
 
细胞类型
B 细胞
 
种属
人
 
样本来源
Whole Blood
 
筛选方法
Negative
 
应用
细胞分选
 
品牌
EasySep，RoboSep
 
研究领域
嵌合体，HLA，免疫
 

查看更多 显示更少

实验数据

Typical EasySep™ HLA B Cell Enrichment Profile

Figure 1. Typical EasySep™ HLA B Cell Enrichment Profile

Starting with HetaSep™-treated whole blood, the CD19+ cell content of the enriched fraction typically ranges from 81.5 - 99.7% (gated on CD45+ cells). Starting with 0.5 mL of HetaSep™-treated whole blood (equivalent to 5 mL of whole blood), 1.2 - 9.1 x 10e5 CD19+ cells are typically recovered.

FACS Data for Anti-Human CD19 Antibody, Clone HIB19, APC-Conjugated

Figure 2. FACS Data for Anti-Human CD19 Antibody, Clone HIB19, APC-Conjugated

Flow cytometry analysis of human whole blood nucleated cells processed with the EasySep™ HLA B Cell Enrichment: Complete Processing Kit for Whole Blood (Catalog #19954HLA) and labeled with Anti-Human CD19 Antibody, Clone HIB19, APC (Catalog # 60005AZ). Histograms show labeling of HetaSep™-treated whole blood cells (Start) and isolated cells (Isolated). Labeling of start cells with a mouse IgG1, kappa APC isotype control antibody is shown (open histogram).

产品说明书及文档

请在《产品说明书》中查找相关支持信息和使用说明，或浏览下方更多实验方案。

Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet
Product Name
EasySep™ HLA B Cell Enrichment: Complete Processing Kit for Whole Blood
Catalog #
19954HLA
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
RoboSep™ HLA B Cell Enrichment: Complete Processing Kit for Whole Blood with Filter Tips
Catalog #
19954HLARF
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Product Name
EasySep™ HLA B Cell Enrichment: Complete Processing Kit for Whole Blood
Catalog #
19954HLA
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Product Name
EasySep™ HLA B Cell Enrichment: Complete Processing Kit for Whole Blood
Catalog #
19954HLA
Lot #
All
Language
English
Document Type
Safety Data Sheet 3
Product Name
EasySep™ HLA B Cell Enrichment: Complete Processing Kit for Whole Blood
Catalog #
19954HLA
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Product Name
RoboSep™ HLA B Cell Enrichment: Complete Processing Kit for Whole Blood with Filter Tips
Catalog #
19954HLARF
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Product Name
RoboSep™ HLA B Cell Enrichment: Complete Processing Kit for Whole Blood with Filter Tips
Catalog #
19954HLARF
Lot #
All
Language
English
Document Type
Safety Data Sheet 3
Product Name
RoboSep™ HLA B Cell Enrichment: Complete Processing Kit for Whole Blood with Filter Tips
Catalog #
19954HLARF
Lot #
All
Language
English
Document Type
Safety Data Sheet 4
Product Name
RoboSep™ HLA B Cell Enrichment: Complete Processing Kit for Whole Blood with Filter Tips
Catalog #
19954HLARF
Lot #
All
Language
English

应用领域

本产品专为以下研究领域设计，适用于工作流程中的高亮阶段。探索这些工作流程，了解更多我们为各研究领域提供的其他配套产品。

Research Area
Workflow Stages

相关材料与文献

技术资料 (8)

EasySep™ Cell Separation Technology
Brochure
EasySep™ Cell Separation Technology
Cell Separation Products for HLA Analysis
Brochure
Cell Separation Products for HLA Analysis
Frequencies of Human Cell Types in Blood-Related Sources
Wallchart
Frequencies of Human Cell Types in Blood-Related Sources
Human Immune Cytokines
Wallchart
Human Immune Cytokines
Isolate Cells with a Simple Pour-Off: EasySep™ Cell Separation Technology
1:13
Video
Isolate Cells with a Simple Pour-Off: EasySep™ Cell Separation Technology
Automate Cell Isolation with the RoboSep™-S Cell Separation Instrument
1:41
Video
Automate Cell Isolation with the RoboSep™-S Cell Separation Instrument
Cell Enrichment of Specific Lymphocyte Populations (T Cells, B Cells, Total Lymphocytes) from Whole Blood
Scientific Poster
Cell Enrichment of Specific Lymphocyte Populations (T Cells, B Cells, Total Lymphocytes) from Whole ...
Evaluation of the Robosep Cell Separation for HLA analysis
Scientific Poster
Evaluation of the Robosep Cell Separation for HLA analysis

常见问题

Can EasySep™ be used for either positive or negative selection?

Yes. The EasySep™ kits use either a negative selection approach by targeting and removing unwanted cells or a positive selection approach targeting desired cells. Depletion kits are also available for the removal of cells with a specific undesired marker (e.g. GlyA).

How does the separation work?

Magnetic particles are crosslinked to cells using Tetrameric Antibody Complexes (TAC). When placed in the EasySep™ Magnet, labeled cells migrate to the wall of the tube. The unlabeled cells are then poured off into a separate fraction.

Which columns do I use?

The EasySep™ procedure is column-free. That's right - no columns!

How can I analyze the purity of my enriched sample?

The Product Information Sheet provided with each EasySep™ kit contains detailed staining information.

Can EasySep™ separations be automated?

Yes. RoboSep™, the fully automated cell separator, automates all EasySep™ labeling and cell separation steps.

Can EasySep™ be used to isolate rare cells?

Yes. We recommend a cell concentration of 2x108 cells/mL and a minimum working volume of 100 µL. Samples containing 2x107 cells or fewer should be suspended in 100 µL of buffer.

Are the EasySep™ magnetic particles FACS-compatible?

Yes, the EasySep™ particles are flow cytometry-compatible, as they are very uniform in size and about 5000X smaller than other commercially available magnetic beads used with column-free systems.

Can the EasySep™ magnetic particles be removed after enrichment?

No, but due to the small size of these particles, they will not interfere with downstream applications.

Can I alter the separation time in the magnet?

Yes; however, this may impact the kit's performance. The provided EasySep™ protocols have already been optimized to balance purity, recovery and time spent on the isolation.

For positive selection, can I perform more than 3 separations to increase purity?

Yes, the purity of targeted cells will increase with additional rounds of separations; however, cell recovery will decrease.

How does the binding of the EasySep™ magnetic particle affect the cells? is the function of positively selected cells altered by the bound particles?

Hundreds of publications have used cells selected with EasySep™ positive selection kits for functional studies. Our in-house experiments also confirm that selected cells are not functionally altered by the EasySep™ magnetic particles.

If particle binding is a key concern, we offer two options for negative selection. The EasySep™ negative selection kits can isolate untouched cells with comparable purities, while RosetteSep™ can isolate untouched cells directly from whole blood without using particles or magnets.

更多信息

更多信息
种属 Human
Magnet Compatibility • EasySep™ Magnet (Catalog #18000) • “The Big Easy” EasySep™ Magnet (Catalog #18001) • Easy 50 EasySep™ Magnet (Catalog #18002) • EasyEights™ EasySep™ Magnet (Catalog #18103) • RoboSep™-S (Catalog #21000)
样本来源 Whole Blood
Selection Method Negative
标记抗体
  1. 抗人CD19抗体，克隆HIB19
    抗人CD19抗体，克隆HIB19

    抗人、黑猩猩CD19的小鼠单克隆IgG1抗体

质量保证:

产品仅供研究使用，不用于针对人或动物的诊断或治疗。 欲获悉更多关于STEMCELL的质控信息，请访问 STEMCELL.CN/COMPLIANCE.
Copyright © 2025 by STEMCELL Technologies. All rights reserved.