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冻存的人外周血B细胞

冻存的人原代细胞

产品号 #(选择产品)

产品号 #70023_C

冻存的人原代细胞

总览

通过免疫磁珠负选从单核细胞(MNC)中分离人外周血原代B细胞(PB)。细胞未被标记,可立即用于下游应用。PB采集过程中添加酸-柠檬酸-葡萄糖溶液A作为抗凝剂(ACDA)。

细胞采集均通过机构审查委员会(IRB)批准的知情同意书及方案进行。

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欲了解更多信息,请浏览有关原代细胞的常见问题解答(FAQs)

包含
• CryoStor® CS10
 
亚型
冻存
 
细胞类型
B 细胞
 
种属

 
细胞和组织来源
外周血
 
供体状态
Normal
 
纯度
流式细胞术检测:CD19+ ≥ 85%
 

实验数据

Figure 1. Cryopreserved B Cells Show High Viability and Produce IgG Antibodies Upon Stimulation

(A) B Cells (Catalog #70023) cryopreserved in CryoStor® CS10 (Catalog #07930) show high viability upon thawing (average = 96.3 ± 0.6 %, n = 6). (B) B cells were cultured for 1 week in RPMI 1640 Medium (Catalog #36750) with 10% FBS, 2mM L-Glutamine (Catalog #07100), 10mM HEPES (Catalog #07200) and 55 µM β-mercaptoethanol and either left unstimulated (control) or stimulated with CD40 in the presence of IL-21 (activated). Activated B cells produce significantly more IgG antibodies compared to unstimulated controls as measured by ELISA.

产品说明书及文档

请在《产品说明书》中查找相关支持信息和使用说明,或浏览下方更多实验方案。

Document Type
Product Name
Catalog #
Lot #
Language
Catalog #
70023, 70023.1
Lot #
All
Language
English

应用领域

本产品专为以下研究领域设计,适用于工作流程中的高亮阶段。探索这些工作流程,了解更多我们为各研究领域提供的其他配套产品。

相关材料与文献

技术资料 (4)

文献 (1)

Discovery of GS-9973, a selective and orally efficacious inhibitor of spleen tyrosine kinase. Currie KS et al. Journal of medicinal chemistry 2014 MAY

Abstract

Spleen tyrosine kinase (Syk) is an attractive drug target in autoimmune, inflammatory, and oncology disease indications. The most advanced Syk inhibitor, R406, 1 (or its prodrug form fostamatinib, 2), has shown efficacy in multiple therapeutic indications, but its clinical progress has been hampered by dose-limiting adverse effects that have been attributed, at least in part, to the off-target activities of 1. It is expected that a more selective Syk inhibitor would provide a greater therapeutic window. Herein we report the discovery and optimization of a novel series of imidazo[1,2-a]pyrazine Syk inhibitors. This work culminated in the identification of GS-9973, 68, a highly selective and orally efficacious Syk inhibitor which is currently undergoing clinical evaluation for autoimmune and oncology indications.

更多信息

更多信息
种属 Human
Contains • CryoStor® CS10
纯度 ≥ 85% CD19+ by flow cytometry
细胞与组织来源 Peripheral Blood
Donor Status Normal
质量保证:

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