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冻存的人外周血CD19+ B细胞

冻存的人原代细胞

产品号 #(选择产品)

产品号 #70033_C

冻存的人原代细胞

总览

通过免疫磁珠正选从外周血单核细胞(PB)中分离原代人CD19+ B细胞。PB采集过程中添加酸-柠檬酸-葡萄糖溶液A作为抗凝剂(ACDA)。

细胞采集均通过机构审查委员会(IRB)批准的知情同意书及方案进行。

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包含
• CryoStor® CS10
 
亚型
冻存
 
细胞类型
B 细胞
 
种属

 
细胞和组织来源
外周血
 
供体状态
Normal
 
纯度
流式细胞术检测:CD19+ ≥ 90%
 

产品说明书及文档

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Document Type
Product Name
Catalog #
Lot #
Language
Catalog #
70033
Lot #
All
Language
English

应用领域

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相关材料与文献

技术资料 (4)

文献 (1)

Paediatric patients with acute leukaemia and KMT2A (MLL) rearrangement show a distinctive expression pattern of histone deacetylases. N. Vega-Garc\'ia et al. British journal of haematology 2018 AUG

Abstract

Histone deacetylase inhibitors (HDACi) had emerged as promising drugs in leukaemia, but their toxicity due to lack of specificity limited their use. Therefore, there is a need to elucidate the role of HDACs in specific settings. The study of HDAC expression in childhood leukaemia could help to choose more specific HDACi for selected candidates in a personalized approach. We analysed HDAC1-11, SIRT1, SIRT7, MEF2C and MEF2D mRNA expression in 211 paediatric patients diagnosed with acute leukaemia. There was a global overexpression of HDACs, while specific HDACs correlated with clinical and biological features, and some even predicted outcome. Thus, some HDAC and MEF2C profiles probably reflected the lineage and the maturation of the blasts and some profiles identified specific oncogenic pathways active in the leukaemic cells. Specifically, we identified a distinctive signature for patients with KMT2A (MLL) rearrangement, with high HDAC9 and MEF2D expression, regardless of age, KMT2A partner and lineage. Moreover, we observed an adverse prognostic value of HDAC9 overexpression, regardless of KMT2A rearrangement. Our results provide useful knowledge on the complex picture of HDAC expression in childhood leukaemia and support the directed use of specific HDACi to selected paediatric patients with acute leukaemia.

更多信息

更多信息
种属 Human
Contains • CryoStor® CS10
纯度 ≥ 90% CD19+ by flow cytometry
细胞与组织来源 Peripheral Blood
Donor Status Normal
质量保证:

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