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ThawSTAR® CFT2全自动细胞解冻系统

全自动细胞解冻系统,确保一致的解冻性能

产品号 #(选择产品)

产品号 #100-0650_C

全自动细胞解冻系统,确保一致的解冻性能

产品优势

  • 使用标准化的解冻方案获得可重复的解冻曲线
  • 避免水浴解冻方案带来的污染风险
  • 可在生物安全柜中直接解冻细胞
  • 配有处理和运输冻存管的便携式解决方案
  • 方便、小巧、灵活度高
  • 利用ThawSTAR® CFT2测试样品可记录仪器性能并生成审计跟踪

产品组分包括

  • Thawstar® CFT2全自动细胞解冻系统,1台(产品号 #100-0640)
  • ThawSTAR® CFT2转运盒,1个(产品号 #100-0641)
  • ThawSTAR® CFT2电源和适配器(产品号 #100-0644)
  • ThawSTAR® CFT2测试样品管,2管

总览

"通过使用ThawSTAR® CFT2全自动细胞解冻系统,提高细胞解冻实验成功率,确保样品无菌性且解冻性能一致性高。ThawSTAR®采用标准化解冻流程,取代手动水浴解冻,避免了样品污染的风险,并提供受控的解冻曲线。这款方便紧凑的全自动细胞解冻系统,允许用户直接在生物安全柜里解冻细胞。只需插入冻存管,并在解冻周期结束时(大约2.5分钟)设备发出提示音后取出即可。ThawSTAR® CFT2全自动细胞解冻系统可以在研发阶段早期使用,并放大到到商业化生产中。

在解冻样品运输过程中,使用ThawSTAR® CFT2转运盒保护细胞免受瞬时升温事件的影响,并确保细胞处于最优的健康状态。它为处理和运输冻存管提供了便携式解决方案,适用于从长期储存在气相液氮或-80°C冰箱中,到使用ThawSTAR® CFT2全自动细胞解冻系统进行下游处理。

ThawSTAR® CFT2全自动细胞解冻系统与多种1.8 - 2.0 mL冻存管兼容。

注:如需性能认证文件和功能测试所需的配件,请使用ThawSTAR® CFT2 IOPQ检测试剂盒(可单独购买)。此外,用于评估仪器性能的ThawSTAR® CFT2测试样品管也可单独购买。"

应用
冻存
 
品牌
ThawSTAR
 
研究领域
脐带血库,免疫
 

实验数据

Bar graphs comparing the number of live cells recovered and percentage viability for peripheral blood mononuclear cells (PBMCs) thawed using ThawSTAR® CFT2 Automated Thawing System versus a water bath.

Figure 1. Frozen PBMCs Thawed Using the ThawSTAR® CFT2 Automated Thawing System Show High Recovery and Viability

Peripheral blood mononuclear cells (PBMCs) cryopreserved in CryoStor® CS10 (Catalog #07930) at a concentration of 3 x 107 cells/vial were retrieved from liquid nitrogen, one week after storage. When thawed using the ThawSTAR® CFT2 Automated Thawing System or a water bath, (A) the mean live cell recovery was 2.78 x 107 vs. 2.96 x 107 cells, respectively and (B) the mean viability was 92.8% vs. 93.9%, respectively. The cells were from 3 different donors and were tested in triplicates. Cell recovery and viability was assessed using a Nucleoview counter.

Bar graphs comparing the number of live cells recovered and percentage viability for monocytes thawed using ThawSTAR® CFT2 Automated Thawing System versus a water bath.

Figure 2. Frozen Monocytes Thawed Using the ThawSTAR® CFT2 Automated Thawing System Show High Recovery and Viability

Monocytes cryopreserved in CryoStor® CS10 (Catalog #07930) at a concentration of 2 x 107 cells/vial were retrieved from liquid nitrogen, one week after storage. When thawed using the ThawSTAR® CFT2 Automated Thawing System or a water bath, (A) the mean live cell recovery was 2.05 x 107 vs. 1.89 x 107 cells, respectively and (B) the mean viability was 95% vs. 94.6%, respectively. The cells were from 3 different donors and were tested in triplicates. Cell recovery and viability was assessed using a Nucleoview counter.

Bar graphs comparing the number of live cells recovered and percentage viability for human pluripotent stem cells (hPSCs) thawed using ThawSTAR® CFT2 Automated Thawing System versus a water bath.

Figure 3. Frozen hPSCs Thawed Using the ThawSTAR® CFT2 Automated Thawing System Show High Recovery and Viability

Human pluripotent stem cells (hPSCs) cryopreserved in CryoStor® CS10 (Catalog #07930) at a concentration of 1 x 106 cells/vial were retrieved from liquid nitrogen, one week after storage. When thawed using the ThawSTAR® CFT2 Automated Thawing System or a water bath, (A) the mean live cell recovery was 9.05 x 105 vs. 9.35 x 105 cells, respectively and (B) the mean viability was 83.04% vs. 82.93%, respectively. The hPSCs were from 3 different cell lines (M001, 1C, and H9) and were tested in triplicates. Cell recovery and viability was assessed using a Nucleoview counter.

产品说明书及文档

请在《产品说明书》中查找相关支持信息和使用说明,或浏览下方更多实验方案。

Document Type
Product Name
Catalog #
Lot #
Language
Catalog #
100-0650
Lot #
All
Language
English
Catalog #
100-0650
Lot #
All
Language
English
Document Type
Safety Data Sheet
Catalog #
100-0650
Lot #
All
Language
English

应用领域

本产品专为以下研究领域设计,适用于工作流程中的高亮阶段。探索这些工作流程,了解更多我们为各研究领域提供的其他配套产品。

质量保证:

产品仅供研究使用,不用于针对人或动物的诊断或治疗。 欲获悉更多关于STEMCELL的质控信息,请访问 STEMCELL.CN/COMPLIANCE.
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