ThawSTAR® CFT2 IOPQ试剂盒

包含安装、操作和性能验证文档及配件，以方便对ThawSTAR® CFT2进行功能测试

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产品号 #（选择产品）

产品号 #100-0730_C

包含安装、操作和性能验证文档及配件，以方便对ThawSTAR® CFT2进行功能测试

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总览

实验数据

产品说明书及文档

应用领域

总览

ThawSTAR® CFT2 IOPQ试剂盒包含安装、操作和性能验证文档以及测试工具配件，方便对ThawSTAR® CFT2全自动细胞解冻系统进行功能测试。ThawSTAR® CFT2全自动细胞解冻系统提供无水标准化细胞解冻流程，配备ThawSTAR® CFT2转运盒、两个ThawSTAR® CFT2测试样品管以及ThawSTAR® CFT2电源和适配器。

使用ThawSTAR® CFT2 IOPQ试剂盒，用户可以获得基于GxP行业标准和GAMP 5方案的分步IOPQ方案和摘要报告模板。

如需了解更多关于使用ThawSTAR®进行细胞解冻方案的信息，请参阅技术手册.

应用
冻存

品牌
ThawSTAR

研究领域
脐带血库，免疫

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实验数据

Bar graphs comparing the number of live cells recovered and percentage viability for peripheral blood mononuclear cells (PBMCs) thawed using ThawSTAR® CFT2 Automated Thawing System versus a water bath.

Figure 1. Frozen PBMCs Thawed Using the ThawSTAR® CFT2 Automated Thawing System Show High Recovery and Viability

Peripheral blood mononuclear cells (PBMCs) cryopreserved in CryoStor® CS10 (Catalog #07930) at a concentration of 3 x 10⁷ cells/vial were retrieved from liquid nitrogen, one week after storage. When thawed using the ThawSTAR® CFT2 Automated Thawing System or a water bath, (A) the mean live cell recovery was 2.78 x 10⁷ vs. 2.96 x 10⁷ cells, respectively and (B) the mean viability was 92.8% vs. 93.9%, respectively. The cells were from 3 different donors and were tested in triplicates. Cell recovery and viability was assessed using a Nucleoview counter.

Bar graphs comparing the number of live cells recovered and percentage viability for monocytes thawed using ThawSTAR® CFT2 Automated Thawing System versus a water bath.

Figure 2. Frozen Monocytes Thawed Using the ThawSTAR® CFT2 Automated Thawing System Show High Recovery and Viability

Monocytes cryopreserved in CryoStor® CS10 (Catalog #07930) at a concentration of 2 x 10⁷ cells/vial were retrieved from liquid nitrogen, one week after storage. When thawed using the ThawSTAR® CFT2 Automated Thawing System or a water bath, (A) the mean live cell recovery was 2.05 x 10⁷ vs. 1.89 x 10⁷ cells, respectively and (B) the mean viability was 95% vs. 94.6%, respectively. The cells were from 3 different donors and were tested in triplicates. Cell recovery and viability was assessed using a Nucleoview counter.

Bar graphs comparing the number of live cells recovered and percentage viability for human pluripotent stem cells (hPSCs) thawed using ThawSTAR® CFT2 Automated Thawing System versus a water bath.

Figure 3. Frozen hPSCs Thawed Using the ThawSTAR® CFT2 Automated Thawing System Show High Recovery and Viability

Human pluripotent stem cells (hPSCs) cryopreserved in CryoStor® CS10 (Catalog #07930) at a concentration of 1 x 10⁶ cells/vial were retrieved from liquid nitrogen, one week after storage. When thawed using the ThawSTAR® CFT2 Automated Thawing System or a water bath, (A) the mean live cell recovery was 9.05 x 10⁵ vs. 9.35 x 10⁵ cells, respectively and (B) the mean viability was 83.04% vs. 82.93%, respectively. The hPSCs were from 3 different cell lines (M001, 1C, and H9) and were tested in triplicates. Cell recovery and viability was assessed using a Nucleoview counter.