ThawSTAR® CFT2测试样品管

用于每次解冻之前和之后记录仪器性能和审计跟踪

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产品号 #（选择产品）

产品号 #100-0643_C

用于每次解冻之前和之后记录仪器性能和审计跟踪

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总览

实验数据

产品说明书及文档

应用领域

总览

使用ThawSTAR® CFT2测试样品管记录ThawSTAR® CFT2全自动细胞解冻系统的性能。在与待解冻生物样本相同的条件下使用 ThawSTAR® CFT2测试样品管，评估并记录每次解冻前后的解冻性能，并创建审计追踪。为了方便起见，ThawSTAR® CFT2测试样品管可单独购买，也可以作为ThawSTAR® CFT2全自动细胞解冻系统的一部分购买。ThawSTAR® CFT2全自动细胞解冻系统配有两个ThawSTAR® CFT2测试样品管，ThawSTAR® CFT2转运盒，ThawSTAR® CFT2电源和适配器，可实现无水标准化细胞解冻流程。如需获取性能鉴定文件和功能测试所需的配件，请使用ThawSTAR® CFT2 IOPQ试剂盒（可单独购买）。

有关使用ThawSTAR®进行细胞解冻方案的更多信息，请浏览技术手册.

应用
冻存

品牌
ThawSTAR

研究领域
脐带血库，免疫

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实验数据

Bar graphs comparing the number of live cells recovered and percentage viability for peripheral blood mononuclear cells (PBMCs) thawed using ThawSTAR® CFT2 Automated Thawing System versus a water bath.

Figure 1. Frozen PBMCs Thawed Using the ThawSTAR® CFT2 Automated Thawing System Show High Recovery and Viability

Peripheral blood mononuclear cells (PBMCs) cryopreserved in CryoStor® CS10 (Catalog #07930) at a concentration of 3 x 10⁷ cells/vial were retrieved from liquid nitrogen, one week after storage. When thawed using the ThawSTAR® CFT2 Automated Thawing System or a water bath, (A) the mean live cell recovery was 2.78 x 10⁷ vs. 2.96 x 10⁷ cells, respectively and (B) the mean viability was 92.8% vs. 93.9%, respectively. The cells were from 3 different donors and were tested in triplicates. Cell recovery and viability was assessed using a Nucleoview counter.

Bar graphs comparing the number of live cells recovered and percentage viability for monocytes thawed using ThawSTAR® CFT2 Automated Thawing System versus a water bath.

Figure 2. Frozen Monocytes Thawed Using the ThawSTAR® CFT2 Automated Thawing System Show High Recovery and Viability

Monocytes cryopreserved in CryoStor® CS10 (Catalog #07930) at a concentration of 2 x 10⁷ cells/vial were retrieved from liquid nitrogen, one week after storage. When thawed using the ThawSTAR® CFT2 Automated Thawing System or a water bath, (A) the mean live cell recovery was 2.05 x 10⁷ vs. 1.89 x 10⁷ cells, respectively and (B) the mean viability was 95% vs. 94.6%, respectively. The cells were from 3 different donors and were tested in triplicates. Cell recovery and viability was assessed using a Nucleoview counter.

Bar graphs comparing the number of live cells recovered and percentage viability for human pluripotent stem cells (hPSCs) thawed using ThawSTAR® CFT2 Automated Thawing System versus a water bath.

Figure 3. Frozen hPSCs Thawed Using the ThawSTAR® CFT2 Automated Thawing System Show High Recovery and Viability

Human pluripotent stem cells (hPSCs) cryopreserved in CryoStor® CS10 (Catalog #07930) at a concentration of 1 x 10⁶ cells/vial were retrieved from liquid nitrogen, one week after storage. When thawed using the ThawSTAR® CFT2 Automated Thawing System or a water bath, (A) the mean live cell recovery was 9.05 x 10⁵ vs. 9.35 x 10⁵ cells, respectively and (B) the mean viability was 83.04% vs. 82.93%, respectively. The hPSCs were from 3 different cell lines (M001, 1C, and H9) and were tested in triplicates. Cell recovery and viability was assessed using a Nucleoview counter.