ThawSTAR® CFT2转运盒

用于处理和运输来自液氮或-80°C 冷冻柜的冻存管的便携式解决方案

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产品号 #100-0642_C

用于处理和运输来自液氮或-80°C 冷冻柜的冻存管的便携式解决方案

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实验数据

产品说明书及文档

应用领域

总览

保护细胞免受瞬时升温事件的影响，维持最大限度的细胞健康状态；在冻存管运输过程中使用ThawSTAR® CFT2转运盒。

该转运盒是一种便携式解决方案，用于处理和运输冻存管，从在气相液氮或 -80°C 冰箱中的长期储存，到使用ThawSTAR® CFT2全自动细胞解冻系统进行下游处理。ThawSTAR® CFT2全自动细胞解冻系统提供无水标准化细胞解冻流程，配有ThawSTAR® CFT2转运盒，两个ThawSTAR® CFT2测试样品管，以及ThawSTAR® CFT2电源和适配器。对于性能鉴定文件和功能测试所需的配件，请使用ThawSTAR® CFT2 IOPQ试剂盒（可单独购买）。

有关使用ThawSTAR®进行细胞解冻方案的更多信息，请浏览技术手册和教育材料."

应用
冻存

品牌
ThawSTAR

研究领域
脐带血库，免疫

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实验数据

Bar graphs comparing the number of live cells recovered and percentage viability for peripheral blood mononuclear cells (PBMCs) thawed using ThawSTAR® CFT2 Automated Thawing System versus a water bath.

Figure 1. Frozen PBMCs Thawed Using the ThawSTAR® CFT2 Automated Thawing System Show High Recovery and Viability

Peripheral blood mononuclear cells (PBMCs) cryopreserved in CryoStor® CS10 (Catalog #07930) at a concentration of 3 x 10⁷ cells/vial were retrieved from liquid nitrogen, one week after storage. When thawed using the ThawSTAR® CFT2 Automated Thawing System or a water bath, (A) the mean live cell recovery was 2.78 x 10⁷ vs. 2.96 x 10⁷ cells, respectively and (B) the mean viability was 92.8% vs. 93.9%, respectively. The cells were from 3 different donors and were tested in triplicates. Cell recovery and viability was assessed using a Nucleoview counter.

Bar graphs comparing the number of live cells recovered and percentage viability for monocytes thawed using ThawSTAR® CFT2 Automated Thawing System versus a water bath.

Figure 2. Frozen Monocytes Thawed Using the ThawSTAR® CFT2 Automated Thawing System Show High Recovery and Viability

Monocytes cryopreserved in CryoStor® CS10 (Catalog #07930) at a concentration of 2 x 10⁷ cells/vial were retrieved from liquid nitrogen, one week after storage. When thawed using the ThawSTAR® CFT2 Automated Thawing System or a water bath, (A) the mean live cell recovery was 2.05 x 10⁷ vs. 1.89 x 10⁷ cells, respectively and (B) the mean viability was 95% vs. 94.6%, respectively. The cells were from 3 different donors and were tested in triplicates. Cell recovery and viability was assessed using a Nucleoview counter.

Bar graphs comparing the number of live cells recovered and percentage viability for human pluripotent stem cells (hPSCs) thawed using ThawSTAR® CFT2 Automated Thawing System versus a water bath.

Figure 3. Frozen hPSCs Thawed Using the ThawSTAR® CFT2 Automated Thawing System Show High Recovery and Viability

Human pluripotent stem cells (hPSCs) cryopreserved in CryoStor® CS10 (Catalog #07930) at a concentration of 1 x 10⁶ cells/vial were retrieved from liquid nitrogen, one week after storage. When thawed using the ThawSTAR® CFT2 Automated Thawing System or a water bath, (A) the mean live cell recovery was 9.05 x 10⁵ vs. 9.35 x 10⁵ cells, respectively and (B) the mean viability was 83.04% vs. 82.93%, respectively. The hPSCs were from 3 different cell lines (M001, 1C, and H9) and were tested in triplicates. Cell recovery and viability was assessed using a Nucleoview counter.