产品号 #100-0642_C
用于处理和运输来自液氮或-80°C 冷冻柜的冻存管的便携式解决方案
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保护细胞免受瞬时升温事件的影响,维持最大限度的细胞健康状态;在冻存管运输过程中使用ThawSTAR® CFT2转运盒。
该转运盒是一种便携式解决方案,用于处理和运输冻存管,从在气相液氮或 -80°C 冰箱中的长期储存,到使用ThawSTAR® CFT2全自动细胞解冻系统进行下游处理。ThawSTAR® CFT2全自动细胞解冻系统提供无水标准化细胞解冻流程,配有ThawSTAR® CFT2转运盒,两个ThawSTAR® CFT2测试样品管,以及ThawSTAR® CFT2电源和适配器。对于性能鉴定文件和功能测试所需的配件,请使用ThawSTAR® CFT2 IOPQ试剂盒(可单独购买)。
有关使用ThawSTAR®进行细胞解冻方案的更多信息,请浏览技术手册和教育材料."
应用
冻存
品牌
ThawSTAR
研究领域
脐带血库,免疫
Figure 1. Frozen PBMCs Thawed Using the ThawSTAR® CFT2 Automated Thawing System Show High Recovery and Viability
Peripheral blood mononuclear cells (PBMCs) cryopreserved in CryoStor® CS10 (Catalog #07930) at a concentration of 3 x 107 cells/vial were retrieved from liquid nitrogen, one week after storage. When thawed using the ThawSTAR® CFT2 Automated Thawing System or a water bath, (A) the mean live cell recovery was 2.78 x 107 vs. 2.96 x 107 cells, respectively and (B) the mean viability was 92.8% vs. 93.9%, respectively. The cells were from 3 different donors and were tested in triplicates. Cell recovery and viability was assessed using a Nucleoview counter.
Figure 2. Frozen Monocytes Thawed Using the ThawSTAR® CFT2 Automated Thawing System Show High Recovery and Viability
Monocytes cryopreserved in CryoStor® CS10 (Catalog #07930) at a concentration of 2 x 107 cells/vial were retrieved from liquid nitrogen, one week after storage. When thawed using the ThawSTAR® CFT2 Automated Thawing System or a water bath, (A) the mean live cell recovery was 2.05 x 107 vs. 1.89 x 107 cells, respectively and (B) the mean viability was 95% vs. 94.6%, respectively. The cells were from 3 different donors and were tested in triplicates. Cell recovery and viability was assessed using a Nucleoview counter.
Figure 3. Frozen hPSCs Thawed Using the ThawSTAR® CFT2 Automated Thawing System Show High Recovery and Viability
Human pluripotent stem cells (hPSCs) cryopreserved in CryoStor® CS10 (Catalog #07930) at a concentration of 1 x 106 cells/vial were retrieved from liquid nitrogen, one week after storage. When thawed using the ThawSTAR® CFT2 Automated Thawing System or a water bath, (A) the mean live cell recovery was 9.05 x 105 vs. 9.35 x 105 cells, respectively and (B) the mean viability was 83.04% vs. 82.93%, respectively. The hPSCs were from 3 different cell lines (M001, 1C, and H9) and were tested in triplicates. Cell recovery and viability was assessed using a Nucleoview counter.
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用于每次解冻之前和之后记录仪器性能和审计跟踪
全自动细胞解冻系统,确保一致的解冻性能
包含安装、操作和性能验证文档及配件,以方便对ThawSTAR® CFT2进行功能测试
无菌、自立式、圆底、内螺纹、带盖聚丙烯低温小瓶
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