产品号 #100-0643_C
用于每次解冻之前和之后记录仪器性能和审计跟踪
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使用ThawSTAR® CFT2测试样品管记录ThawSTAR® CFT2全自动细胞解冻系统的性能。在与待解冻生物样本相同的条件下使用 ThawSTAR® CFT2测试样品管,评估并记录每次解冻前后的解冻性能,并创建审计追踪。为了方便起见,ThawSTAR® CFT2测试样品管可单独购买,也可以作为ThawSTAR® CFT2全自动细胞解冻系统的一部分购买。ThawSTAR® CFT2全自动细胞解冻系统配有两个ThawSTAR® CFT2测试样品管,ThawSTAR® CFT2转运盒,ThawSTAR® CFT2电源和适配器,可实现无水标准化细胞解冻流程。如需获取性能鉴定文件和功能测试所需的配件,请使用ThawSTAR® CFT2 IOPQ试剂盒(可单独购买)。
有关使用ThawSTAR®进行细胞解冻方案的更多信息,请浏览技术手册.
应用
冻存
品牌
ThawSTAR
研究领域
脐带血库,免疫
Figure 1. Frozen PBMCs Thawed Using the ThawSTAR® CFT2 Automated Thawing System Show High Recovery and Viability
Peripheral blood mononuclear cells (PBMCs) cryopreserved in CryoStor® CS10 (Catalog #07930) at a concentration of 3 x 107 cells/vial were retrieved from liquid nitrogen, one week after storage. When thawed using the ThawSTAR® CFT2 Automated Thawing System or a water bath, (A) the mean live cell recovery was 2.78 x 107 vs. 2.96 x 107 cells, respectively and (B) the mean viability was 92.8% vs. 93.9%, respectively. The cells were from 3 different donors and were tested in triplicates. Cell recovery and viability was assessed using a Nucleoview counter.
Figure 2. Frozen Monocytes Thawed Using the ThawSTAR® CFT2 Automated Thawing System Show High Recovery and Viability
Monocytes cryopreserved in CryoStor® CS10 (Catalog #07930) at a concentration of 2 x 107 cells/vial were retrieved from liquid nitrogen, one week after storage. When thawed using the ThawSTAR® CFT2 Automated Thawing System or a water bath, (A) the mean live cell recovery was 2.05 x 107 vs. 1.89 x 107 cells, respectively and (B) the mean viability was 95% vs. 94.6%, respectively. The cells were from 3 different donors and were tested in triplicates. Cell recovery and viability was assessed using a Nucleoview counter.
Figure 3. Frozen hPSCs Thawed Using the ThawSTAR® CFT2 Automated Thawing System Show High Recovery and Viability
Human pluripotent stem cells (hPSCs) cryopreserved in CryoStor® CS10 (Catalog #07930) at a concentration of 1 x 106 cells/vial were retrieved from liquid nitrogen, one week after storage. When thawed using the ThawSTAR® CFT2 Automated Thawing System or a water bath, (A) the mean live cell recovery was 9.05 x 105 vs. 9.35 x 105 cells, respectively and (B) the mean viability was 83.04% vs. 82.93%, respectively. The hPSCs were from 3 different cell lines (M001, 1C, and H9) and were tested in triplicates. Cell recovery and viability was assessed using a Nucleoview counter.
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用于处理和运输来自液氮或-80°C 冷冻柜的冻存管的便携式解决方案
全自动细胞解冻系统,确保一致的解冻性能
包含安装、操作和性能验证文档及配件,以方便对ThawSTAR® CFT2进行功能测试
无菌、自立式、圆底、内螺纹、带盖聚丙烯低温小瓶
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