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EasySep™人中央和效应记忆CD4+ T细胞分选试剂盒

采用磁珠解离技术的免疫磁珠细胞分选试剂盒
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产品号 #（选择产品）

产品号 #17865_C

采用磁珠解离技术的免疫磁珠细胞分选试剂盒

产品优势

  • 快捷、操作简单且无需分离柱
  • 纯度可高达96%
  • 免清洗即可去除EasySep™ Releasable RapidSpheres™可解离磁珠

产品组分包括

  • EasySep™人中央和效应记忆CD4+ T细胞分选试剂盒（产品号，#17865）
    • EasySep™人记忆CD4+ T细胞分选抗体混合物，0.5mL
    • EasySep™ Dextran RapidSpheres™磁珠,1mL
    • EasySep™人CD62L正选抗体混合物,1mL
    • EasySep™人CD25去除抗体混合物, 1mL
    • EasySep™ Releasable RapidSpheres™ 50201磁珠, 1mL
    • EasySep™ Release缓冲液, 1mL
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  1. EasySep™ Magnet
    EasySep™ Magnet

    Magnet for column-free immunomagnetic separation

  2. EasySep™ Buffer
    EasySep™ Buffer

    Cell separation buffer

总览
实验数据
产品说明书及文档
应用领域
相关材料与文献
相关产品

总览

本试剂盒可从新鲜外周血单个核细胞(PBMCs)或洗涤后的白细胞单采术样本中分选出人

CD62L+CD45RO+和CD62L-CD45RO+记忆CD4+ T细胞。首先，通过使用抗体复合物和

EasySep™ Releasable RapidSpheres™的免疫磁珠正选来分选CD62L+细胞。接着，结合在EasySep™分离的CD62L+细胞上的磁珠被解离，并通过抗体复合物和EasySep™ Dextran RapidSpheres™去除CD45RA+和非CD4+ T细胞。分选后的组分含有高纯度的CD62L+CD45RO+中央记忆T细胞，可立即用于下游应用。效应记忆CD4+ T细胞可从CD62L-组分中分离获得。

CD62L+CD45RO+和CD62L-CD45RO+记忆CD4+ T细胞。首先，通过使用抗体复合物和

EasySep™ Releasable RapidSpheres™的免疫磁珠正选来分选CD62L+细胞。接着，结合在EasySep™分离的CD62L+细胞上的磁珠被解离，并通过抗体复合物和EasySep™ Dextran RapidSpheres™去除CD45RA+和非CD4+ T细胞。分选后的组分含有高纯度的CD62L+CD45RO+中央记忆T细胞，可立即用于下游应用。效应记忆CD4+ T细胞可从CD62L-组分中分离获得。

CD62L+CD45RO+和CD62L-CD45RO+记忆CD4+ T细胞。首先，通过使用抗体复合物和

EasySep™ Releasable RapidSpheres™的免疫磁珠正选来分选CD62L+细胞。接着，结合在EasySep™分离的CD62L+细胞上的磁珠被解离，并通过抗体复合物和EasySep™ Dextran RapidSpheres™去除CD45RA+和非CD4+ T细胞。分选后的组分含有高纯度的CD62L+CD45RO+中央记忆T细胞，可立即用于下游应用。效应记忆CD4+ T细胞可从CD62L-组分中分离获得。

CD62L+CD45RO+和CD62L-CD45RO+记忆CD4+ T细胞。首先，通过使用抗体复合物和

EasySep™ Releasable RapidSpheres™的免疫磁珠正选来分选CD62L+细胞。接着，结合在EasySep™分离的CD62L+细胞上的磁珠被解离，并通过抗体复合物和EasySep™ Dextran RapidSpheres™去除CD45RA+和非CD4+ T细胞。分选后的组分含有高纯度的CD62L+CD45RO+中央记忆T细胞，可立即用于下游应用。效应记忆CD4+ T细胞可从CD62L-组分中分离获得。

磁体兼容性
• EasySep™ Magnet (Catalog #18000) • “The Big Easy” EasySep™ Magnet (Catalog #18001) • Easy 50 EasySep™ Magnet (Catalog #18002) • EasyEights™ EasySep™ Magnet (Catalog #18103)
 
亚型
细胞分选试剂盒
 
细胞类型
T 细胞，CD4+
 
种属
人
 
样本来源
Leukapheresis，PBMC
 
筛选方法
Positive
 
应用
细胞分选
 
品牌
EasySep
 
研究领域
免疫
 

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实验数据

Starting with fresh PBMCs, the central memory CD4+ T cell content (CD3+CD4+CD45RO+CD62L+) of the isolated fraction is typically 92.3 ± 3.9% (mean ± SD using the purple EasySep™ magnet). The effector memory content (CD3+CD4+CD45RO+CD62L-) of the isolated fraction is typically 92.4 ± 4.1% (mean ± SD).
NOTE: RBCs were removed from the start sample by lysis prior to flow cytometry.

产品说明书及文档

请在《产品说明书》中查找相关支持信息和使用说明，或浏览下方更多实验方案。

Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet
Product Name
EasySep™ Human Central and Effector Memory CD4+ T Cell Isolation Kit
Catalog #
17865
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Product Name
EasySep™ Human Central and Effector Memory CD4+ T Cell Isolation Kit
Catalog #
17865
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Product Name
EasySep™ Human Central and Effector Memory CD4+ T Cell Isolation Kit
Catalog #
17865
Lot #
All
Language
English
Document Type
Safety Data Sheet 3
Product Name
EasySep™ Human Central and Effector Memory CD4+ T Cell Isolation Kit
Catalog #
17865
Lot #
All
Language
English
Document Type
Safety Data Sheet 4
Product Name
EasySep™ Human Central and Effector Memory CD4+ T Cell Isolation Kit
Catalog #
17865
Lot #
All
Language
English
Document Type
Safety Data Sheet 5
Product Name
EasySep™ Human Central and Effector Memory CD4+ T Cell Isolation Kit
Catalog #
17865
Lot #
All
Language
English
Document Type
Safety Data Sheet 6
Product Name
EasySep™ Human Central and Effector Memory CD4+ T Cell Isolation Kit
Catalog #
17865
Lot #
All
Language
English
Document Type
Safety Data Sheet 7
Product Name
EasySep™ Human Central and Effector Memory CD4+ T Cell Isolation Kit
Catalog #
17865
Lot #
All
Language
English

应用领域

本产品专为以下研究领域设计，适用于工作流程中的高亮阶段。探索这些工作流程，了解更多我们为各研究领域提供的其他配套产品。

Research Area
Workflow Stages

更多信息

更多信息
种属 Human
Magnet Compatibility • EasySep™ Magnet (Catalog #18000) • “The Big Easy” EasySep™ Magnet (Catalog #18001) • Easy 50 EasySep™ Magnet (Catalog #18002) • EasyEights™ EasySep™ Magnet (Catalog #18103)
样本来源 Leukapheresis, PBMC
Selection Method Positive
标记抗体
  1. 抗人CD45RO抗体，克隆UCHL1
    抗人CD45RO抗体，克隆UCHL1

    小鼠单克隆IgG2a抗体，抗人、黑猩猩、普通狨猴CD45RO

  2. 抗人CD45抗体，克隆HI30
    抗人CD45抗体，克隆HI30

    小鼠单克隆IgG1抗体，抗人、黑猩猩CD45

质量保证:

产品仅供研究使用，不用于针对人或动物的诊断或治疗。 欲获悉更多关于STEMCELL的质控信息，请访问 STEMCELL.CN/COMPLIANCE.
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