产品号 #02034_C
用于人白细胞介素13的检测和测定
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用于人白细胞介素13的检测和测定
人白细胞介素13 (IL-13)酶联免疫吸附测定试剂盒是专为定量检测和测量白细胞介素13在生物液体,如血清,血浆和细胞培养上清。IL-13由Th2细胞、肥大细胞和NK细胞分泌。IL-13参与促进B细胞增殖,诱导类转换到IgG4和IgE,招募和激活产生IgE的B细胞,激活JAK/STAT和IRS-1和IRS-2途径。
该检测基于夹心ELISA方法,将样品添加到酶联免疫吸附试验(ELISA)带材板上,该带材板上预先涂有捕获的细胞因子特异性抗体。捕获的细胞因子通过添加生物素化检测抗体进行检测,然后添加链亲和素-辣根过氧化物酶(SA-HRP),该酶与生物素化抗体结合。添加显色酶底物3,3 ‘,5,5 ’四甲基联苯胺(TMB)可得到着色产物,其强度与样品中细胞因子的浓度成正比。细胞因子的浓度是通过与平行分析的细胞因子标准品的连续稀释进行比较来确定的。
Subtype
Complete Kits
Cell Type
Granulocytes and Subsets, NK Cells, T Cells, CD8+
Species
Human
Area of Interest
Immunology
Representative Standard Curve
• Reportable Range: 3.2 - 1000 pg/mL. This is the concentration range in which measurement of the analyte can be done with the highest precision, accuracy, and linearity.
• Sensitivity: The limit of detection of this assay is 1 pg/mL. This is the analyte concentration with absorbance two standard deviations higher than the zero standard.
• Accuracy: The analyte standard of this ELISA was calibrated against NIBSC international standard 94/622.
• Recovery: A mid-curve recovery of 85 - 121% was determined by spiking defined amounts of analyte standard into serum or plasma samples in repeated experiments.
• Precision: The intra-assay precision of this assay is 3% (CV). The inter-assay precision of this assay is 14.9% (CV).
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种属 | Human |
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全血免疫磁珠负选试剂盒
杜氏磷酸盐缓冲液,不含钙和镁离子
小鼠抗人、恒河猴、食蟹猴CD16单克隆IgG1抗体
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