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人IL-13酶联免疫吸附测定试剂盒

用于人白细胞介素13的检测和测定

产品号 #(选择产品)

产品号 #02034_C

用于人白细胞介素13的检测和测定

产品组分包括

  • 人IL-13酶联免疫吸附测定试剂盒,2板(目录#02034)
    • 人白细胞介素-13 (IL-13)酶联免疫吸附试验板
    • 人IL-13标准品,1瓶
    • Human IL-13 Biotinylated Detection Antibody, 30µL
    • Sa-hrp, 30µl
    • 标准重组缓冲液B, 1ml
    • 洗涤缓冲液(20X), 120 mL
    • ELISA稀释液,120 mL
    • SA-HRP稀释液,25ml
    • TMB底物,25ml
    • 停止液,25ml
    • 粘接板盖,6个
  • 人IL-13酶联免疫吸附测定试剂盒,10板(目录#02035)
    • 人IL-13酶联免疫吸附试验板,10x
    • 人IL-13标准品,1瓶
    • Human IL-13 Biotinylated Detection Antibody, 130µL
    • Sa-hrp, 130µl
    • 标准重组缓冲液B, 1ml
    • 洗涤缓冲液(20倍),5 × 120毫升
    • ELISA稀释剂,3 × 120ml
    • SA-HRP稀释剂,120 mL
    • TMB底物,120 mL
    • 停止液,120ml
    • 胶粘剂板盖,30x

总览

人白细胞介素13 (IL-13)酶联免疫吸附测定试剂盒是专为定量检测和测量白细胞介素13在生物液体,如血清,血浆和细胞培养上清。IL-13由Th2细胞、肥大细胞和NK细胞分泌。IL-13参与促进B细胞增殖,诱导类转换到IgG4和IgE,招募和激活产生IgE的B细胞,激活JAK/STAT和IRS-1和IRS-2途径。

该检测基于夹心ELISA方法,将样品添加到酶联免疫吸附试验(ELISA)带材板上,该带材板上预先涂有捕获的细胞因子特异性抗体。捕获的细胞因子通过添加生物素化检测抗体进行检测,然后添加链亲和素-辣根过氧化物酶(SA-HRP),该酶与生物素化抗体结合。添加显色酶底物3,3 ‘,5,5 ’四甲基联苯胺(TMB)可得到着色产物,其强度与样品中细胞因子的浓度成正比。细胞因子的浓度是通过与平行分析的细胞因子标准品的连续稀释进行比较来确定的。

Subtype
Complete Kits
 
Cell Type
Granulocytes and Subsets, NK Cells, T Cells, CD8+
 
Species
Human
 
Area of Interest
Immunology
 

实验数据

Representative Standard Curve

• Reportable Range: 3.2 - 1000 pg/mL. This is the concentration range in which measurement of the analyte can be done with the highest precision, accuracy, and linearity.
• Sensitivity: The limit of detection of this assay is 1 pg/mL. This is the analyte concentration with absorbance two standard deviations higher than the zero standard.
• Accuracy: The analyte standard of this ELISA was calibrated against NIBSC international standard 94/622.
• Recovery: A mid-curve recovery of 85 - 121% was determined by spiking defined amounts of analyte standard into serum or plasma samples in repeated experiments.
• Precision: The intra-assay precision of this assay is 3% (CV). The inter-assay precision of this assay is 14.9% (CV).

产品说明书及文档

请在《产品说明书》中查找相关支持信息和使用说明,或浏览下方更多实验方案。

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02034, 02035
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English
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Safety Data Sheet 1
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02034, 02035
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English
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Safety Data Sheet 2
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02034, 02035
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Safety Data Sheet 3
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02034, 02035
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Safety Data Sheet 4
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02034, 02035
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Safety Data Sheet 5
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02034, 02035
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Safety Data Sheet 6
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02034, 02035
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Safety Data Sheet 7
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02034, 02035
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Safety Data Sheet 8
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02034, 02035
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Safety Data Sheet 9
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02034, 02035
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All
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English
Catalog #
02034, 02035
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All
Language
English

应用领域

本产品专为以下研究领域设计,适用于工作流程中的高亮阶段。探索这些工作流程,了解更多我们为各研究领域提供的其他配套产品。

相关材料与文献

技术资料 (2)

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种属 Human
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