若您需要咨询产品或有任何技术问题,请通过官方电话 400 885 9050 或邮箱 info.cn@stemcell.com 与我们联系

人IL-7ELISA试剂盒

用于人白细胞介素-7的检测和测定

产品号 #(选择产品)

产品号 #100-1139_C

用于人白细胞介素-7的检测和测定

产品组分包括

  • 人IL-7ELISA试剂盒
    • 人IL-7ELISA酶标板
    • 人IL-7 HRP检测抗体,1瓶
    • 人白细胞介素-7标准品,1瓶
    • 洗涤缓冲液(20X), 30ml
    • 稀释缓冲液(20X), 12.5 mL
    • 标准稀释缓冲液,2ml
    • 过氧化物溶液,12.5 mL
    • TMB底物,12.5 mL
    • 终止液,11mL

总览

人白细胞介素 7 (IL-7) ELISA 试剂盒用于定量检测细胞培养上清液中的人 IL-7。IL-7 是一种造血生长因子,由骨髓和胸腺的基质细胞分泌。它通过 IL-7R 发出信号,刺激造血干细胞分化为淋巴祖细胞。IL-7 对 T 细胞稳态至关重要,并促进幼稚 T 细胞和记忆 T 细胞的存活和增殖。

该检测基于夹心 ELISA 方法,将样品加入预先包被有针对人 IL-7 的特异性捕获抗体的 ELISA可拆酶标板。通过添加辣根过氧化物酶 (HRP) 偶联的检测抗体来检测捕获的 IL-7。添加显色酶底物 3,3'5,5' - 四甲基联苯胺 (TMB) 会产生有色产物,其强度与样品中人 IL-7 的浓度成正比。

亚型
完整试剂盒
 
细胞类型
造血干/祖细胞,T 细胞
 
种属

 
应用
ELISAs
 
研究领域
免疫,感染性疾病(传染病)
 

实验数据

Representative Standard Curve Of Human IL-7

Figure 1. Representative Standard Curve Of Human IL-7

The reportable range (the concentration range in which measurement of the analyte can be done with the highest precision, accuracy, and linearity) of this assay is 5.47 - 350 pg/mL. The lower limit of detection (LLD) of this assay is 2.07 pg/mL. This is the analyte concentration with absorbance three standard deviations higher than the zero standard. A mid-curve recovery of 98 - 108% was determined by spiking defined amounts of analyte standard into cell culture supernatant in repeated experiments. The intra-assay precision of this assay is 4.3% (CV). The Inter-assay precision of this assay is 6.2% (CV).

产品说明书及文档

请在《产品说明书》中查找相关支持信息和使用说明,或浏览下方更多实验方案。

Document Type
Product Name
Catalog #
Lot #
Language
Catalog #
100-1139
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Catalog #
100-1139
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Catalog #
100-1139
Lot #
All
Language
English
Document Type
Safety Data Sheet 3
Catalog #
100-1139
Lot #
All
Language
English
Document Type
Safety Data Sheet 4
Catalog #
100-1139
Lot #
All
Language
English
Document Type
Safety Data Sheet 5
Catalog #
100-1139
Lot #
All
Language
English
Document Type
Safety Data Sheet 6
Catalog #
100-1139
Lot #
All
Language
English
Document Type
Safety Data Sheet 7
Catalog #
100-1139
Lot #
All
Language
English
Document Type
Safety Data Sheet 8
Catalog #
100-1139
Lot #
All
Language
English
Document Type
Safety Data Sheet 9
Catalog #
100-1139
Lot #
All
Language
English

更多信息

更多信息
种属 Human
质量保证:

产品仅供研究使用,不用于针对人或动物的诊断或治疗。
Copyright © 2025 by STEMCELL Technologies. All rights reserved.