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人潜伏tgf - β 1酶联免疫吸附测定试剂盒

用于人潜伏转化生长因子- β 1的检测和测定

产品号 #(选择产品)

产品号 #02018_C

用于人潜伏转化生长因子- β 1的检测和测定

产品组分包括

  • 人潜伏tgf - β 1酶联免疫吸附测定试剂盒(2板)(目录#02018)
    • 人潜伏TGF-β1酶联免疫吸附测定板,2x
    • 人TGF-β1标准品,1瓶
    • 人潜伏TGF-β1生物素化检测抗体,50µL
    • Sa-hrp, 30µl
    • 标准重构缓冲液C, 1ml
    • 洗涤缓冲液(20X), 120 mL
    • ELISA稀释液,120 mL
    • SA-HRP稀释液,25ml
    • TMB底物,25ml
    • 停止液,25ml
    • 粘接板盖,6个
  • 人潜伏tgf - β 1酶联免疫吸附测定试剂盒,10板(目录#02019)
    • 人潜伏TGF-β1 ELISA板,10x
    • 人TGF-β1标准品,1瓶
    • 人潜伏TGF-β1生物素化检测抗体,250µL
    • Sa-hrp, 130µl
    • 标准重构缓冲液C, 1ml
    • 洗涤缓冲液(20倍),5 × 120毫升
    • ELISA稀释剂,3 × 120ml
    • SA-HRP稀释剂,120 mL
    • TMB底物,120 mL
    • 停止液,120ml
    • 胶粘剂板盖,30x

总览

人潜伏转化生长因子-β1 (TGF-β1)试剂盒用于血浆、细胞培养上清等生物体液中潜伏TGF-β1的定量检测和测定。TGF-β1主要由血小板和骨骼产生,大多数有核细胞和肿瘤也产生少量TGF-β1。TGF-β1刺激间充质来源的细胞,但抑制上皮或神经外胚层来源的细胞。TGF-β1与潜伏期相关蛋白(Latency Associated Protein, LAP)结合形成潜伏复合物。该试剂盒检测该复合物的LAP实体。

该检测基于夹心ELISA方法,将样品添加到酶联免疫吸附试验(ELISA)带材板上,该带材板上预先涂有捕获的细胞因子特异性抗体。捕获的细胞因子通过添加生物素化的检测抗体来检测,然后添加链霉亲和素-辣根过氧化物酶,该酶与生物素化的抗体结合。添加显色酶底物3,3 ‘,5,5 ’四甲基联苯胺(TMB)可得到着色产物,其强度与样品中细胞因子的浓度成正比。细胞因子的浓度是通过与平行分析的细胞因子标准品的连续稀释进行比较来确定的。

Subtype
Complete Kits
 
Cell Type
Dendritic Cells, Macrophages, Platelets, T Cells, Tumor Cells
 
Species
Human
 
Area of Interest
Immunology
 

实验数据

Representative Standard Curve

• Reportable range: 0.32 - 32 pM. This is the concentration range in which measurement of the analyte can be done with the highest precision, accuracy, and linearity. Recombinant human LAP (latency associated protein) homodimer is the analyte standard of this ELISA. Determination of latent TGF-β1 using the standard curve is based on a molar comparison. 1 pM LAP (54 pg/mL) corresponds to 1 pM latent TGF-β1 (80 pg/mL).
• Sensitivity: The limit of detection of this assay is 0.13 pM. This is the analyte concentration with absorbance two standard deviations higher than the zero standard.
• Recovery: A mid-curve recovery of 100% was determined by dilution of plasma samples in repeated experiments.
• Precision: The intra-assay precision of this assay is 3.0% (CV). The inter-assay precision of this assay is 10.4% (CV).

产品说明书及文档

请在《产品说明书》中查找相关支持信息和使用说明,或浏览下方更多实验方案。

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02018
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English
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Safety Data Sheet 1
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02018
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Safety Data Sheet 2
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02018
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Safety Data Sheet 3
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02018
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Safety Data Sheet 4
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02018
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Safety Data Sheet 5
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02018
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Safety Data Sheet 6
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Safety Data Sheet 7
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02018
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Safety Data Sheet 8
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02018
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English
Document Type
Safety Data Sheet 9
Catalog #
02018
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English
Catalog #
02018
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All
Language
English

应用领域

本产品专为以下研究领域设计,适用于工作流程中的高亮阶段。探索这些工作流程,了解更多我们为各研究领域提供的其他配套产品。

相关材料与文献

技术资料 (2)

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种属 Human
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