人重组Noggin（大肠杆菌表达），ACF

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产品号 #（选择产品）

产品号 #100-1731_C

无动物成分

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实验数据

产品说明书及文档

应用领域

总览

Noggin可用于支持体外未分化的人胚胎干细胞的维持，并在短期内防止自发分化（Chaturvedi et al. Cell Prolif, 2009）。Noggin能与转化生长因子β (TGF-β)家族的骨形态发生蛋白（BMP）配体结合，并对其起到拮抗作用。Noggin对外胚层衍生结构、胚胎体、骨骼模式和神经发生的发育至关重要。它还参与软骨形成、成骨和关节形成（Krause et al. Int J Biochem Cell Biol, 2011)，并促进胚胎干细胞向多巴胺能神经元的分化并增强这些多巴胺能神经元的存活。（Chiba et al. Stem Cells, 2008）。

亚型
细胞因子

别名
NOG，SYM1，SYNS1

细胞类型
神经细胞，PSC衍生，神经元，多能干细胞

种属
人

研究领域
神经科学，干细胞生物学

制剂类别
Animal Component-Free

纯度
≥98%

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实验数据

Figure 1. Biological Activity and Molecular Mass of Human Recombinant Noggin (E. coli-expressed), ACF

(A) The biological activity of Human Recombinant Noggin (E. coli-expressed), ACF was tested by its ability to inhibit BMP-2 activity in HEK293T cells. Inhibition of BMP-2 was measured using a firefly luciferase reporter assay. Cells were treated with a serial dilution of Noggin and standard concentration of BMP-2 for 6 hours. The EC50 is defined as the effective concentration of the growth factor at which inhibition of BMP-2 activity is at 50% of maximum. The EC50 in the above example is 1.5 nM (70.1 ng/mL). (B) 7 μg of Human Recombinant Noggin (E. coli-expressed), ACF was resolved with SDS-PAGE under reducing (+) conditions and non-reducing (-) conditions and visualized by Coomassie Blue staining. Human Recombinant Noggin has a predicted molecular mass of 23 kDa in reducing conditions.