EasySep™小鼠TIL（CD45）正选试剂盒
产品号 #72512_C
激活素/BMP/TGF-β通路激活剂
若您需要咨询产品或有任何技术问题，请通过官方电话 400 885 9050 或邮箱 info.cn@stemcell.com 与我们联系
总览
定形内胚层诱导剂 1 (IDE1) 通过激活 SMAD2 磷酸化和 NODAL 表达诱导小鼠或人多能干细胞（PSCs）的分化（Borowiak et al.）。EC₅₀ = 125 nM 时，SOX17 在小鼠 ES 细胞中被诱导表达。
分化
·在缺乏激活素 A、NODAL 或饲养层细胞的情况下，诱导小鼠或人 ES 细胞向定形内胚层分化（Borowiak et al.）。
细胞类型
内胚层，PSC衍生，多能干细胞
种属
人，小鼠，非人灵长类，其它细胞系，大鼠
应用
分化
研究领域
上皮细胞研究，干细胞生物学
CAS 编号
1160927-48-9
化学式
C₁₅H₁₈N₂O₅
纯度
≥ 95 %
通路
Activin/Nodal/TGFβ
产品说明书及文档
请在《产品说明书》中查找相关支持信息和使用说明，或浏览下方更多实验方案。
应用领域
本产品专为以下研究领域设计，适用于工作流程中的高亮阶段。探索这些工作流程，了解更多我们为各研究领域提供的其他配套产品。
相关材料与文献
技术资料 (2)
文献 (1)
Small molecules efficiently direct endodermal differentiation of mouse and human embryonic stem cells. Cell stem cell 2009 APR
Abstract
An essential step for therapeutic and research applications of stem cells is the ability to differentiate them into specific cell types. Endodermal cell derivatives, including lung, liver, and pancreas, are of interest for regenerative medicine, but efforts to produce these cells have been met with only modest success. In a screen of 4000 compounds, two cell-permeable small molecules were indentified that direct differentiation of ESCs into the endodermal lineage. These compounds induce nearly 80% of ESCs to form definitive endoderm, a higher efficiency than that achieved by Activin A or Nodal, commonly used protein inducers of endoderm. The chemically induced endoderm expresses multiple endodermal markers, can participate in normal development when injected into developing embryos, and can form pancreatic progenitors. The application of small molecules to differentiate mouse and human ESCs into endoderm represents a step toward achieving a reproducible and efficient production of desired ESC derivatives.
更多信息
| 种属 | Human, Mouse, Non-Human Primate, Other, Rat |
|---|---|
| Cas Number | 1160927-48-9 |
| Chemical Formula | C₁₅H₁₈N₂O₅ |
| 纯度 | ≥ 95% |
| Pathway | Activin/Nodal/TGFβ |
相关产品
-
STEMdiff™ Definitive Endoderm KitDefined animal component-free medium for the differentiation of human ES and iPS cells to definitive endoderm
-
STEMdiff™ Pancreatic Progenitor KitSerum-free medium for the differentiation of human ES and iPS cells to pancreatic progenitor cells
-
(-) -Indolactam V（吲哚内酰胺 V)蛋白激酶C（PKC）激活剂
-
IDE2激活素/BMP/TGF-β通路激活剂
