产品号 #05120_C
Serum-free medium for the differentiation of human ES and iPS cells to pancreatic progenitor cells
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Serum-free medium for the differentiation of human ES and iPS cells to pancreatic progenitor cells
STEMdiff™ Pancreatic Progenitor Kit is a serum-free medium that supports efficient and reproducible generation of pancreatic progenitor cells from human pluripotent stem cells (hPSCs). Cells are differentiated through four stages: 1) definitive endoderm, 2) primitive gut tube, 3) posterior foregut endoderm, and 4) pancreatic progenitor cells. The differentiated cells express key markers of pancreatic progenitor cells, including PDX-1, NKX6.1 and SOX9 and up-regulation of insulin and glucagon. The resulting pancreatic progenitor cells can be further differentiated to insulin-producing beta cells or other endocrine and exocrine pancreatic cell fates.
STEMdiff™ Pancreatic Progenitor Kit has been optimized for differentiation of cells maintained in mTeSR™1 (Catalog #85850) or mTeSR™ Plus (Catalog #100-0276).
亚型
专用培养基
细胞类型
胰腺细胞,多能干细胞
种属
人
应用
细胞培养,分化
品牌
STEMdiff
研究领域
癌症,疾病建模,上皮细胞研究,干细胞生物学
制剂类别
无血清
Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.
This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.
We get a really great efficiency of differentiation in several pluripotent stem cell lines determined by expression of key markers: upregulation of PDX1, SOX9, FOXA2 and GATA4, and down-regulation of Sox17. We can generate these pancreatic progenitor cells reproducibly and efficiently; variability within the protocol is low.
Drs. Jamie Trott and Ray Dunn, A*STAR Institute of Medical Biology (IMB), Singapore
种属 | Human |
---|---|
配方类别 | Serum-Free |