EasySep™小鼠TIL（CD45）正选试剂盒
产品号 #05445
用于人间充质干细胞的无动物成分培养基
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要查看实验方案所需的所有配套产品，请参阅《实验方案与技术文档》。
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Animal Component-Free Cell Dissociation KitDissociation kit for human stem and progenitor cells
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L-GlutamineCell culture supplement
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D-PBS (Without Ca++ and Mg++)Dulbecco’s phosphate-buffered saline without calcium and magnesium
总览
使用这款无动物成分(ACF)且不含细胞外囊泡(EV)的培养基，可降低人间充质基质细胞(MSC，亦称间充质干细胞)培养的差异性，提升实验可重复性。MesenCult™-ACF Plus培养基经过优化，无需血清即可从骨髓或脂肪组织等多种来源衍生人类MSC。
与含血清或EV去除的含血清培养基相比，使用本试剂盒培养的MSC扩增效率更高（增殖速率与累计细胞总量），且功能不受影响。培养细胞表达典型的MSC表面标志物，并保持强劲的扩增速率和三系分化能力。
MesenCult™-ACF Plus培养基专为MSC的衍生、扩增、冻存以及将人多能干细胞分化为间充质祖细胞而设计——专为高效稳定的MSC培养而优化。
为实现无动物成分的优化的细胞冻存目的，推荐使用MesenCult™-ACF冻存培养基冻存先前在MesenCult™系列培养基（包括MesenCult™-ACF Plus）中培养的人类MSC。有关相关产品的完整列表，包括可用的分化培养基，请访问 our MSC area of interest page，或通过 techsupport@stemcell.com 联系我们获取。
注意：MesenCult™-ACF Plus完全培养基须添加L-谷氨酰胺。该产品需配合无动物成分细胞贴附基质使用，后者作为MesenCult™-ACF Plus培养试剂盒的组分提供。
CollPlant是细胞贴附基质中重组人胶原蛋白(rhCollagen)组分的生产商。
与含血清或EV去除的含血清培养基相比，使用本试剂盒培养的MSC扩增效率更高（增殖速率与累计细胞总量），且功能不受影响。培养细胞表达典型的MSC表面标志物，并保持强劲的扩增速率和三系分化能力。
MesenCult™-ACF Plus培养基专为MSC的衍生、扩增、冻存以及将人多能干细胞分化为间充质祖细胞而设计——专为高效稳定的MSC培养而优化。
为实现无动物成分的优化的细胞冻存目的，推荐使用MesenCult™-ACF冻存培养基冻存先前在MesenCult™系列培养基（包括MesenCult™-ACF Plus）中培养的人类MSC。有关相关产品的完整列表，包括可用的分化培养基，请访问 our MSC area of interest page，或通过 techsupport@stemcell.com 联系我们获取。
注意：MesenCult™-ACF Plus完全培养基须添加L-谷氨酰胺。该产品需配合无动物成分细胞贴附基质使用，后者作为MesenCult™-ACF Plus培养试剂盒的组分提供。
CollPlant是细胞贴附基质中重组人胶原蛋白(rhCollagen)组分的生产商。
与含血清或EV去除的含血清培养基相比，使用本试剂盒培养的MSC扩增效率更高（增殖速率与累计细胞总量），且功能不受影响。培养细胞表达典型的MSC表面标志物，并保持强劲的扩增速率和三系分化能力。
MesenCult™-ACF Plus培养基专为MSC的衍生、扩增、冻存以及将人多能干细胞分化为间充质祖细胞而设计——专为高效稳定的MSC培养而优化。
为实现无动物成分的优化的细胞冻存目的，推荐使用MesenCult™-ACF冻存培养基冻存先前在MesenCult™系列培养基（包括MesenCult™-ACF Plus）中培养的人类MSC。有关相关产品的完整列表，包括可用的分化培养基，请访问 our MSC area of interest page，或通过 techsupport@stemcell.com 联系我们获取。
注意：MesenCult™-ACF Plus完全培养基须添加L-谷氨酰胺。该产品需配合无动物成分细胞贴附基质使用，后者作为MesenCult™-ACF Plus培养试剂盒的组分提供。
CollPlant是细胞贴附基质中重组人胶原蛋白(rhCollagen)组分的生产商。
亚型
专用培养基
细胞类型
间充质细胞，PSC衍生，间充质干/祖细胞
种属
人
应用
细胞培养，扩增，培养
品牌
MesenCult
研究领域
细胞外囊泡研究，干细胞生物学
制剂类别
Animal Component-Free，无血清
实验数据
产品说明书及文档
请在《产品说明书》中查找相关支持信息和使用说明，或浏览下方更多实验方案。
应用领域
本产品专为以下研究领域设计，适用于工作流程中的高亮阶段。探索这些工作流程，了解更多我们为各研究领域提供的其他配套产品。
相关材料与文献
技术资料 (12)
文献 (8)
Despite mutation acquisition in hematopoietic stem cells, JMML-propagating cells are not always restricted to this compartment. Leukemia 2020 jun
Abstract
Juvenile myelomonocytic leukemia (JMML) is a rare aggressive myelodysplastic/myeloproliferative neoplasm of early childhood, initiated by RAS-activating mutations. Genomic analyses have recently described JMML mutational landscape; however, the nature of JMML-propagating cells (JMML-PCs) and the clonal architecture of the disease remained until now elusive. Combining genomic (exome, RNA-seq), Colony forming assay and xenograft studies, we detect the presence of JMML-PCs that faithfully reproduce JMML features including the complex/nonlinear organization of dominant/minor clones, both at diagnosis and relapse. Further integrated analysis also reveals that although the mutations are acquired in hematopoietic stem cells, JMML-PCs are not always restricted to this compartment, highlighting the heterogeneity of the disease during the initiation steps. We show that the hematopoietic stem/progenitor cell phenotype is globally maintained in JMML despite overexpression of CD90/THY-1 in a subset of patients. This study shed new lights into the ontogeny of JMML, and the identity of JMML-PCs, and provides robust models to monitor the disease and test novel therapeutic approaches.
CD81 Controls Beige Fat Progenitor Cell Growth and Energy Balance via FAK Signaling. Cell 2020 jun
Abstract
Adipose tissues dynamically remodel their cellular composition in response to external cues by stimulating beige adipocyte biogenesis; however, the developmental origin and pathways regulating this process remain insufficiently understood owing to adipose tissue heterogeneity. Here, we employed single-cell RNA-seq and identified a unique subset of adipocyte progenitor cells (APCs) that possessed the cell-intrinsic plasticity to give rise to beige fat. This beige APC population is proliferative and marked by cell-surface proteins, including PDGFR$\alpha$, Sca1, and CD81. Notably, CD81 is not only a beige APC marker but also required for de novo beige fat biogenesis following cold exposure. CD81 forms a complex with $\alpha$V/$\beta$1 and $\alpha$V/$\beta$5 integrins and mediates the activation of integrin-FAK signaling in response to irisin. Importantly, CD81 loss causes diet-induced obesity, insulin resistance, and adipose tissue inflammation. These results suggest that CD81 functions as a key sensor of external inputs and controls beige APC proliferation and whole-body energy homeostasis.
Precision installation of a highly efficient suicide gene safety switch in human induced pluripotent stem cells. Stem cells translational medicine 2020 jul
Abstract
Human pluripotent stem cells including induced pluripotent stem cells (iPSCs) and embryonic stem cells hold great promise for cell-based therapies, but safety concerns that complicate consideration for routine clinical use remain. Installing a safety switch" based on the inducible caspase-9 (iCASP9) suicide gene system should offer added control over undesirable cell replication or activity. Previous studies utilized lentiviral vectors to integrate the iCASP9 system into T cells and iPSCs. This method results in random genomic insertion of the suicide switch and inefficient killing of the cells after the switch is "turned on" with a small molecule (eg AP1903). To improve the safety and efficiency of the iCASP9 system for use in iPSC-based therapy we precisely installed the system into a genomic safe harbor the AAVS1 locus in the PPP1R12C gene. We then evaluated the efficiencies of different promoters to drive iCASP9 expression in human iPSCs. We report that the commonly used EF1$\alpha$ promoter is silenced in iPSCs and that the endogenous promoter of the PPP1R12C gene is not strong enough to drive high levels of iCASP9 expression. However the CAG promoter induces strong and stable iCASP9 expression in iPSCs and activation of this system with AP1903 leads to rapid killing and complete elimination of iPSCs and their derivatives including MSCs and chondrocytes in vitro. Furthermore iPSC-derived teratomas shrank dramatically or were completely eliminated after administration of AP1903 in mice. Our data suggest significant improvements on existing iCASP9 suicide switch technologies and may serve as a guide to other groups seeking to improve the safety of stem cell-based therapies."
更多信息
| 种属 | Human |
|---|---|
| 配方类别 | Animal Component-Free, Serum-Free |
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