STEMdiff™ 间充质祖细胞试剂盒

总览

使用STEMdiff™间充质祖细胞试剂盒，可高效且可重复地从人多能干细胞（hPSCs）中诱导生成间充质祖细胞（MPCs）。这款完全无动物成分（ACF）的试剂盒提供从人胚胎干细胞（ESCs）或诱导多能干细胞（iPSCs）诱导和扩增MPCs所需的所有试剂。

使用该试剂盒生成的细胞表达标志性的间充质标志物（CD73、CD90、CD105），当与MesenCult™分化试剂盒配合使用时，表现出强效扩增以及向脂肪生成、成骨和软骨生成谱系的分化能力。与原代间充质基质细胞（MSCs）相比，使用该工作流程衍生的MPCs表现出降低的异质性，并为再生医学研究、疾病建模和药物发现提供了一致且可重复的平台。

有关MSC的进一步分化和培养，请查看我们的MesenCult™ MSC细胞培养产品组合，其中包含人iPSC衍生的间充质祖细胞，以便您使用经过验证的纯MSC细胞培养物开始您的实验。

CollPlant是细胞贴附基质中重组人胶原蛋白（rhCollagen）成分的制造商。

本产品仅供研究使用。如需将本产品用于任何临床或商业用途，请联系STEMCELL。

分类
专用培养基

细胞类型
间充质细胞，PSC衍生

种属
人

应用
细胞培养，分化

品牌
STEMdiff

研究领域
药物发现和毒性检测，干细胞生物学

制剂类别
不含动物成分，无血清

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实验数据

Human ES- and iPS-derived MPCs Can Be Further Differentiated Into Adipogenic, Chondrogenic and Osteogenic Lineages

Figure 1. Schematic of Differentiation Protocol and Timeline

In Phase 1, human embryonic stem cells (hESCs) or human induced pluripotent stem cells (hiPSCs) are cultured in mTeSR™1, mTeSR™Plus, or TeSR™-E8™ medium. On Day 0 (Phase 2) of the protocol, cells are ready for induction into early mesoderm progenitor cells by replacing TeSR™ medium with STEMdiff™-ACF Mesenchymal Induction Medium. By Day 4 (Phase 3), STEMdiff™-ACF Mesenchymal Induction Medium is replaced with MesenCult™-ACF Plus Medium to derive early mesenchymal progenitor cells (MPCs). On Day 6, cells are passaged onto cultureware precoated with MesenCult™-ACF Attachment Substrate in MesenCult™-ACF Plus Medium. By Day 21, hESC- or hiPSC-derived MPCs exhibit the characteristics of MPCs.

Cell Expansion and Doubling Rate of MPCs Derived from Human ES (H9) and iPS (STiPS-F016 and -F031) Cells in MesenCult™-ACF Medium

Figure 2. Cell Expansion and Doubling Rate of MPCs Derived from hESCs (H9) and hiPSCs (STiPS-M001, SCTi003-A, WLS-1C H9 ) in MesenCult™-ACF Plus Medium

(A) Cumulative cell expansion over 12 passages for MPCs derived from hESC and hiPSC lines. (B) Doubling time for hESC- and hiPSC-derived MPCs.

A Representative Flow Cytometric Analysis of STiPS-F016-derived MPCs Expressing Mesenchymal Surface Markers By Day 21

Figure 3. A Representative Flow Cytometric Analysis of STiPS-M001-derived MPCs Expressing Mesenchymal Surface Markers by Day 21

hiPSC-derived MPCs, generated using the STEMdiff™ Mesenchymal Progenitor Kit, express high levels of mesenchymal surface markers (CD73, CD90, and CD105). hESC-derived MPCs display the same phenotype (data not shown).

Figure 4. hESC- and hiPSC-derived MPCs Can Be Further Differentiated into Adipogenic, Chondrogenic and Osteogenic Lineages

(A) MPCs generated from the 21 day protocol (described in Figure 1) and subsequently cultured in MesenCult™-ACF Plus Medium develop MPC-like morphology. MPCs can be differentiated to (B) adipocytes (Oil Red O staining); (C) chondrocytes (Alcian Blue staining); and (D) osteoblasts (Alizarin Red S staining).

产品说明书及文档

请在《产品说明书》中查找相关支持信息和使用说明，或浏览下方更多实验方案。

Document Type

Product Name

Catalog #

Lot #

Language

Document Type

Product Information Sheet

Product Name

STEMdiff™ Mesenchymal Progenitor Kit

Catalog #

05240

Lot #

All

Language

English

Document Type

Safety Data Sheet 1

Product Name

STEMdiff™ Mesenchymal Progenitor Kit

Catalog #

05240

Lot #

All

Language

English

Document Type

Safety Data Sheet 2

Product Name

STEMdiff™ Mesenchymal Progenitor Kit

Catalog #

05240

Lot #

All

Language

English

Document Type

Safety Data Sheet 3

Product Name

STEMdiff™ Mesenchymal Progenitor Kit

Catalog #

05240

Lot #

All

Language

English

Document Type

Safety Data Sheet 4

Product Name

STEMdiff™ Mesenchymal Progenitor Kit

Catalog #

05240

Lot #

All

Language

English

应用领域

本产品专为以下研究领域设计，适用于工作流程中的高亮阶段。探索这些工作流程，了解更多我们为各研究领域提供的其他配套产品。

Research Area

Workflow Stages

Workflow Stages for Human Pluripotent Stem Cell Research

Reprogramming

Maintenance

Differentiation

Workflow Stages for hPSC-Derived Mesoderm Cell Research

Mesoderm Induction

Downstream Differentiation

Workflow Stages for Mesenchymal Stem and Progenitor Cell Research

Cell Sourcing and Isolation

Culture and Cryopreservation

Differentiation

Characterization

相关材料与文献

Autologous iPSC- and MSC-derived chondrocyte implants for cartilage repair in a miniature pig model Lee et al. Stem Cell Research & Therapy 2025 Feb

Abstract

Induced pluripotent stem cell (iPSC)-derived mesenchymal stem cells (iMSCs) have greater potential for generating chondrocytes without hypertrophic and fibrotic phenotypes compared to bone marrow-derived mesenchymal stem/stromal cells (BMSCs). However, there is a lack of research demonstrating the use of autologous iMSCs for repairing articular chondral lesions in large animal models. In this study, we aimed to evaluate the effectiveness of autologous miniature pig (minipig) iMSC-chondrocyte (iMSC-Ch)-laden implants in comparison to autologous BMSC-chondrocyte (BMSC-Ch)-laden implants for cartilage repair in porcine femoral condyles. iMSCs and BMSCs were seeded into fibrin glue/nanofiber constructs and cultured with chondrogenic induction media for 7 days before implantation. To assess the regenerative capacity of the cells, 19 skeletally mature Yucatan minipigs were randomly divided into microfracture control, acellular scaffold, iMSC, and BMSC subgroups. A cylindrical defect measuring 7 mm in diameter and 0.6 mm in depth was created on the articular cartilage surface without violating the subchondral bone. The defects were then left untreated or treated with acellular or cellular implants. Both cellular implant-treated groups exhibited enhanced joint repair compared to the microfracture and acellular control groups. Immunofluorescence analysis yielded significant findings, showing that cartilage treated with iMSC-Ch implants exhibited higher expression of COL2A1 and minimal to no expression of COL1A1 and COL10A1, in contrast to the BMSC-Ch-treated group. This indicates that the iMSC-Ch implants generated more hyaline cartilage-like tissue compared to the BMSC-Ch implants. Our findings contribute to filling the knowledge gap regarding the use of autologous iPSC derivatives for cartilage repair in a translational animal model. Moreover, these results highlight their potential as a safe and effective therapeutic strategy. The online version contains supplementary material available at 10.1186/s13287-025-04215-7.

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Tracing genomic instability in induced mesenchymal stromal cell manufacture: an integration-free transfection approach Experimental & Molecular Medicine 2025 Apr

Abstract

Here we systematically investigated genomic alterations from the initiation of induced pluripotent stem (iPS) cell generation to induced mesenchymal stromal/stem cell differentiation. We observed a total of ten copy number alterations (CNAs) and five single-nucleotide variations (SNVs) during the phases of reprogramming, differentiation and passaging. We identified a higher frequency of CNAs and SNVs in iPS cells generated using the Sendai virus (SV) method compared with those generated with episomal vectors (Epi). Specifically, all SV-iPS cell lines exhibited CNAs during the reprogramming phase, while only 40% of Epi-iPS cells showed such alterations. Additionally, SNVs were observed exclusively in SV-derived cells during passaging and differentiation, with no SNVs detected in Epi-derived lines. Gene expression analysis revealed upregulation of chromosomal instability-related genes in late-passage SV-iPSCs, further indicating increased genomic instability. Notably, TP53 mutations were identified, underscoring the vulnerability of the gene and the critical need for careful genomic scrutiny when preparing iPS cells and derived cell lines. Genomic instability in induced pluripotent stem cells revealedThis study explores the potential of using induced pluripotent stem (iPS) cells to create mesenchymal stem (MS) cells for medical treatments. iPS cells can be reprogrammed from regular cells and can become any cell type, including MS cells, which are important for tissue repair. However, a concern is that iPS cells might develop genetic changes that could affect their safety. Here researchers investigated these genetic changes during the creation and growth of iPS cells and their transformation into MS cells using advanced techniques such as chromosomal microarray and next-generation sequencing, alongside conventional methods. The study found that iPS cells often develop genetic alterations, which can persist as they are turned into MS cells. The results suggest that while iPS cells hold promise for regenerative medicine, careful monitoring of genetic stability is crucial. Future research should focus on improving methods to ensure safety of iPS cell-derived therapies.This summary was initially drafted using artificial intelligence, then revised and fact-checked by the author.

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Mesenchymal Stem Cells Derived from Human Urine-Derived iPSCs Exhibit Low Immunogenicity and Reduced Immunomodulatory Profile International Journal of Molecular Sciences 2024 Sep

Abstract

Human-induced pluripotent stem cell (iPSC)-derived mesenchymal stem cells (iMSCs) represent a promising and renewable cell source for therapeutic applications. A systematic evaluation of the immunological properties and engraftment potential of iMSCs generated from urine-derived iPSCs is lacking, which has impeded their broader application. In this study, we differentiated urine-derived iPSCs into iMSCs and assessed their fundamental MSC characteristics, immunogenicity, immunomodulatory capacity and in vivo engraftment. Compared to umbilical cord-derived MSCs (UCMSCs), iMSCs demonstrated an enhanced proliferative capacity, a higher level of regenerative gene expression, and lower immunogenicity, strengthening resistance to apoptosis induced by allogeneic peripheral blood mononuclear cells (PBMCs) and the NK-92 cell line. In addition, iMSCs exhibited a diminished ability to inhibit T cell proliferation and activation compared with UCMSCs. Transcriptomic analyses further revealed the decreased expression of immune regulatory factors in iMSCs. After transfusion into mouse models, iMSCs engrafted in the lungs, liver, and spleen and exhibited the ability to migrate to tumor tissues. Our results indicated that iMSCs generated from urine-derived iPSCs have a significant replicative capacity, low immunogenicity and unique immunomodulatory properties, and hence offer obvious advantages in immune privilege and allogenic therapeutic application prospects.

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物种	人类
配方	不含动物成分, 无血清

STEMdiff™ 间充质祖细胞试剂盒

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STEMdiff™ 间充质祖细胞试剂盒

产品号 #05240

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产品号 #05240_C

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总览

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产品说明书及文档

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相关材料与文献

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