小鼠IL-2酶联免疫吸附测定试剂盒

总览

小鼠白细胞介素2 （IL-2）酶联免疫吸附测定试剂盒是专为定量检测和测量小鼠白细胞介素2在生物液体，如血清，血浆和细胞培养上清。IL-2主要由活化的CD4+和CD8+ T细胞分泌，但也由B细胞和树突状细胞少量产生。IL-2是T细胞发育的主要调节因子，参与T细胞的生长和扩增，以及调节性T细胞的发育。

该检测基于夹心ELISA方法，将样品添加到酶联免疫吸附试验（ELISA）带材板上，该带材板上预先涂有捕获的细胞因子特异性抗体。捕获的细胞因子通过添加生物素化的检测抗体来检测，然后添加链霉亲和素-辣根过氧化物酶，该酶与生物素化的抗体结合。添加显色酶底物3,3 ‘,5,5 ’四甲基联苯胺（TMB）可得到着色产物，其强度与样品中细胞因子的浓度成正比。细胞因子的浓度是通过与平行分析的细胞因子标准品的连续稀释进行比较来确定的。

Subtype
Complete Kits

Cell Type
B Cells, Dendritic Cells, T Cells, CD4+, T Cells, CD8+

Species
Mouse

Application
ELISA

Area of Interest
Immunology

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实验数据

Representative Standard Curve

• Reportable range: 10 - 1000 pg/mL. This is the concentration range in which measurement of the analyte can be done with the highest precision, accuracy, and linearity.
• Sensitivity: The limit of detection of this assay is 4 pg/mL. This is the analyte concentration with absorbance two standard deviations higher than the zero standard.
• Accuracy: The analyte standard of this ELISA was calibrated against NIBSC* international standard 93/566.
• Recovery: A mid-curve recovery of 106 - 114% was determined by spiking defined amounts of analyte standard into serum or plasma samples in repeated experiments.
• Precision: The intra-assay precision of this assay is 3.1% (CV). The inter-assay precision of this assay is 6.7% (CV).

*National Institute of Biological Standards and Control, Potters Bar, Hertfordshire EN6 3QG, UK.