小鼠IL-2酶联免疫吸附测定试剂盒

总览

小鼠白细胞介素2（IL-2）ELISA试剂盒用于定量检测和测定血清、血浆和细胞培养上清液等生物体液中的小鼠IL-2。IL-2主要由活化的CD4+和CD8+ T细胞分泌，但B细胞和树突状细胞也少量产生。IL-2是T细胞发育的主要调节因子，有助于T细胞的生长和扩增，以及调节性T细胞的发育。

该检测基于夹心ELISA方法，将样品加入预先包被有针对细胞因子特异性捕获抗体的ELISA板中。通过添加生物素化的检测抗体，然后加入与生物素化抗体结合的链霉亲和素-辣根过氧化物酶，即可检测捕获的细胞因子。加入显色酶底物3,3',5,5'-四甲基联苯胺（TMB）后产生显色产物，其强度与样品中细胞因子的浓度成正比。通过与平行分析的细胞因子标准品的梯度稀释液进行比较，可以确定细胞因子的浓度。

分类
完整试剂盒

细胞类型
B 细胞，树突状细胞（DCs），T 细胞，CD4+，T 细胞，CD8+

种属
小鼠

应用
ELISAs

研究领域
免疫

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实验数据

Representative Standard Curve

• Reportable range: 10 - 1000 pg/mL. This is the concentration range in which measurement of the analyte can be done with the highest precision, accuracy, and linearity.
• Sensitivity: The limit of detection of this assay is 4 pg/mL. This is the analyte concentration with absorbance two standard deviations higher than the zero standard.
• Accuracy: The analyte standard of this ELISA was calibrated against NIBSC* international standard 93/566.
• Recovery: A mid-curve recovery of 106 - 114% was determined by spiking defined amounts of analyte standard into serum or plasma samples in repeated experiments.
• Precision: The intra-assay precision of this assay is 3.1% (CV). The inter-assay precision of this assay is 6.7% (CV).

*National Institute of Biological Standards and Control, Potters Bar, Hertfordshire EN6 3QG, UK.