Mouse TNF-alpha ELISA Kit | STEMCELL Technologies

总览

小鼠肿瘤坏死因子-α（TNF-α）ELISA试剂盒用于定量检测和测定血清、血浆和细胞培养上清液等生物体液中的小鼠TNF-α。TNF-α 主要由活化的T细胞和巨噬细胞在炎症或感染后产生，但也由B细胞、NK细胞、肥大细胞、中性粒细胞、树突状细胞、小胶质细胞、内皮细胞、平滑肌细胞、心肌细胞和成纤维细胞产生。TNF-α是一种促炎细胞因子，可在体外刺激抗肿瘤反应。

该检测方法基于夹心ELISA方法，将样品加入预先包被有针对该细胞因子的特异性捕获抗体的ELISA板中。通过添加生物素化的检测抗体，然后加入与生物素化抗体结合的链霉亲和素-辣根过氧化物酶，即可检测捕获的细胞因子。加入显色酶底物3,3',5,5'-四甲基联苯胺（TMB）后产生显色产物，其强度与样品中细胞因子的浓度成正比。通过与平行分析的细胞因子标准品的梯度稀释液进行比较，可以确定细胞因子的浓度。

分类
完整试剂盒

细胞类型
B 细胞，心肌细胞，PSC衍生，树突状细胞（DCs），内皮细胞，粒细胞及其亚群，巨噬细胞，鼠胚胎成纤维细胞，肌源干/祖细胞，NK 细胞，T 细胞

种属
小鼠

应用
ELISAs

研究领域
免疫，干细胞生物学

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实验数据

Representative Standard Curve

• Reportable range: 15.6 - 2000 pg/mL. This is the concentration range in which measurement of the analyte can be done with the highest precision, accuracy, and linearity.
• Sensitivity: The limit of detection of this assay is 5 pg/mL. This is the analyte concentration with absorbance two standard deviations higher than the zero standard.
• Accuracy: The analyte standard of this ELISA was calibrated against NIBSC international standard 88/532.
• Recovery: A mid-curve recovery of 84 - 86% was determined by spiking defined amounts of analyte standard into serum or plasma samples in repeated experiments.
• Precision: The intra-assay precision of this assay is 2.4% (CV). The Inter-assay precision of this assay is 3.7% (CV).

* National Institute of Biological Standards and Control, Potters Bar, Hertfordshire EN6 3QG, UK.