MSC表征抗体组合

人间充质间质细胞多色流式检测试剂盒

率先评论此产品

产品号 #（选择产品）

产品号 #100-0354_C

人间充质间质细胞多色流式检测试剂盒

跳到结尾的图片库

跳转到图像库的开头

要查看实验方案所需的所有配套产品，请参阅《实验方案与技术文档》。

Round-Bottom Polystyrene Tubes with Caps, 5 m...
Sterile polystyrene round-bottom tubes; 5mL with caps
EasySep™ Buffer
Cell separation buffer
Corning® 96-Well Round-Bottom Microplate
Clear polystyrene round-bottom untreated microplate with lid
Anti-Human CD32 Antibody, Clone IV.3
Mouse monoclonal IgG2b antibody against human CD32
DAPI (Hydrochloride)
DNA-labeling dye
Labeling Antibodies
Compatible antibodies for purity assessment of isolated cells

总览

实验数据

产品说明书及文档

应用领域

总览

MSC表征抗体组合设计用于人间充质间质细胞（MSCs）的表型分析。本试剂盒包含三种荧光染料偶联抗体，用于鉴定人MSCs，以及一种荧光染料偶联抗体，用于鉴定non-MSCs。当流式检测时，≥95%的MSC细胞群必须表达CD73、CD90和CD105，并且这些细胞必须缺乏CD45的表达（≤2%阳性）。该抗体组合通过一步法标记来验证MSCs。MSC表征抗体组合可用于鉴定来自各种组织的MSCs，如骨髓来源的MSCs、多能干细胞来源的间充质祖细胞、脂肪来源的MSCs、牙髓来源的MSCs和脐带来源的MSCs。

亚型
一抗

细胞类型
间充质基质细胞

应用
流式细胞术

研究领域
内皮细胞研究，干细胞生物学

查看更多显示更少

实验数据

Flow cytometry dot plots comparing the difference in expression of CD45, CD73, CD90, and CD105 in human bone marrow-derived mesenchymal stromal cells (MSCs) labeled with the hMSC CD45/CD73/CD90/CD105 Antibody Panel and cultured either in MesenCult™-ACF Plus Medium or in serum-containing medium.

Figure 1. Flow Cytometry Analysis of Human Bone Marrow-Derived Mesenchymal Stromal Cells Labeled Using the hMSC CD45/CD73/CD90/CD105 Antibody Panel

(A) When cultured in MesenCult™-ACF Plus Medium, the labeled human bone marrow (BM)-derived mesenchymal stromal cells (MSCs), demonstrated positive expression of CD73, CD105, and CD90 as well as negative expression of CD45 at P0. (B) When cultured in serum-containing medium, the BM-derived MSCs exhibit reduced CD73, CD105, and CD90 expression as well as increased CD45 expression at P0 due to contaminating CD45+ cells.