EasySep™小鼠TIL（CD45）正选试剂盒
产品号 #73292_C
神经分化诱导剂
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总览
Neurodazine是一种细胞渗透性的三取代咪唑和神经发生剂（Williams等）。
分化
·诱导C2C12成肌细胞以及成熟人肌肉细胞的神经发生，以神经基因的上调为标志（Williams等）。
·诱导神经发生并阻止 P19 小鼠胚胎癌细胞的星形胶质细胞分化（Kim等）。
·诱导人（SH-SY5Y）、小鼠（神经-2a）神经母细胞瘤细胞以及小鼠NIH3T3成纤维细胞的神经元分化（Halder等）。
细胞类型
癌细胞及细胞系，肌源干/祖细胞，神经元
种属
人，小鼠，非人灵长类，其它细胞系，大鼠
应用
分化
研究领域
神经科学
CAS 编号
937807-66-4
化学式
C₂₇H₂₁ClN₂O₃
纯度
≥98%
产品说明书及文档
请在《产品说明书》中查找相关支持信息和使用说明，或浏览下方更多实验方案。
应用领域
本产品专为以下研究领域设计，适用于工作流程中的高亮阶段。探索这些工作流程，了解更多我们为各研究领域提供的其他配套产品。
相关材料与文献
技术资料 (1)
文献 (4)
Synthetic small molecules that induce neuronal differentiation in neuroblastoma and fibroblast cells. Molecular bioSystems 2015
Abstract
An investigation was conducted to demonstrate that neurodazine (Nz) and neurodazole (Nzl), two imidazole-based small molecules, promote neuronal differentiation in both neuroblastoma and fibroblast cells. The results show that differentiated cells generated by treatment with Nz and Nzl express neuron-specific markers. The ability of Nz and Nzl to induce neurogenesis of neuroblastoma and fibroblast cells was found to be comparable to those of the known neurogenic factors, retinoic acid and trichostatin A. In addition, the cells differentiated by Nz and Nzl are observed to express different isoforms of glutamate receptors. The results of signaling pathway studies reveal that two substances enhance neurogenesis in neuroblastoma cells by activating Wnt and Shh signaling pathways and neurogenesis in fibroblast cells by mainly activating the Wnt signaling pathway. Observations made in the present study suggest that Nz and Nzl will serve as chemical tools to generate specific populations of neuronal cells from readily available and simply manageable cells.
Imidazole-based small molecules that promote neurogenesis in pluripotent cells. Angewandte Chemie (International ed. in English) 2014
Abstract
Reported herein are two imidazole-based small molecules, termed neurodazine (Nz) and neurodazole (Nzl), which induce neuronal differentiation of pluripotent P19 cells. Their ability to induce neurogenesis of P19 cells is comparable to that of retinoic acid. However, Nz and Nzl were found to be more selective neurogenesis inducers than retinoic acid owing to their unique ability to suppress astrocyte differentiation of P19 cells. Our results also show that Nz and Nzl promote production of physiologically active neurons because P19-cell-derived neurons induced by these substances have functional glutamate responsiveness. The present study suggests that Nz and Nzl could serve as important chemical tools to induce formation of specific populations of neuronal cell types from pluripotent cells.
Fluorescent high-throughput screening of chemical inducers of neuronal differentiation in skeletal muscle cells. Nature protocols 2008
Abstract
This protocol describes detailed procedures for the fluorescent high-throughput screening of small molecules that induce neurogenesis in cultures of skeletal muscle cells. The detection of neurogenesis relies on a fluorescent dye, FM 1-43, which is used to study the neuronal property of depolarization-induced synaptic vesicle recycling. Thus, small molecules with neurogenesis-inducing activity in skeletal muscle cells can be rapidly identified by measuring the fluorescence intensity of the treated cells using a fluorescent microplate reader. This protocol uses murine myoblast C2C12 cells for screening, which are readily available and relatively easy to culture. Neurogenesis of PC12 cells induced by nerve growth factor is employed as a positive control for this screening. The screening time for this protocol is 8 d, which also includes the procedure to detect depolarization-induced synaptic vesicle recycling using FM 1-43.
更多信息
| 种属 | Human, Mouse, Non-Human Primate, Other, Rat |
|---|---|
| Cas Number | 937807-66-4 |
| Chemical Formula | C₂₇H₂₁ClN₂O₃ |
| 纯度 | ≥ 98% |
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