PneumaCult™气道类器官试剂盒

无血清和无bpe培养基用于气道类器官的高效建立和分化

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产品号 #（选择产品）

产品号 #05060_C

无血清和无bpe培养基用于气道类器官的高效建立和分化

产品优势

三维体外系统，概括了人体内气道的关键特征
用于人气道上皮细胞扩增和分化的完整培养基配方，与PneumaCult™-Ex Plus兼容
方便的格式和易于使用的协议，提供一致的结果

产品组分包括

PneumaCult™气道类器官基础培养基，450 mL（目录#05061）
PneumaCult™气道类器官播种补充剂，10 mL（目录#05062）
PneumaCult™气道类器官分化补充剂，40ml（目录#05063）

要查看实验方案所需的所有配套产品，请参阅《实验方案与技术文档》。

总览

实验数据

产品说明书及文档

应用领域

总览

PneumaCult™气道类器官试剂盒是一种不含血清和BPE的培养体系，用于高效地建立和分化源自人支气管上皮细胞（HBECs）的呼吸道类器官。

在PneumaCult™-Ex Plus培养基（#05040）中进行2D扩增后，使用PneumaCult™呼吸道类器官接种培养基启动3D类器官培养，然后使用PneumaCult™呼吸道类器官分化培养基促进类器官形态发育，形成具有呼吸道生理特诊的结构。完全分化的人呼吸道器官再现了体内人呼吸道的关键特性，如被极化的呼吸道上皮细胞层包围的含有纤毛细胞和杯状细胞的空心腔。

呼吸道类器官的应用包括肺部疾病建模、药物筛选（疗效与毒性评估）以及呼吸道上皮功能研究。

浏览我们关于使用PneumaCult™培养呼吸道类器官的常见问题解答（FAQs）。

如果您打算将本产品用于商业目的，请通过www.huborganoids.nl与HUB联系，以获取商业用途许可或HUB许可相关的说明。

分类
专用培养基

细胞类型
气道细胞

种属
人

应用
细胞培养，分化，扩增，类器官培养

品牌
PneumaCult

研究领域
疾病建模，药物发现和毒理检测，上皮细胞研究，类器官，呼吸系统研究

制剂类别
无血清

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实验数据

Schematic of human airway organoid culture workflow using PneumaCult™ Airway Organoid Kit

Figure 1. Overview of the PneumaCult™ Culture System for Human Airway Organoid Generation

In the early two-dimensional expansion phase of the human airway organoid culture procedure, HBECs are expanded using PneumaCult™-Ex Plus Medium. The HBECs are then embedded into a Matrigel® dome and expanded for 4 - 7 days using PneumaCult™ Airway Organoid Seeding Medium. Following the expansion, the HBECs are differentiated using PneumaCult™ Airway Organoid Differentiation Medium for an additional 21+ days.

Fully differentiated human airway organoids generated using PneumaCult™ Airway Organoid Kit display physiologically relevant morphology.

Figure 2. Fully Differentiated Human Airway Organoids Generated Using PneumaCult™ Airway Organoid Kit

(A) Bright-field image of airway organoids growing in PneumaCult™ Airway Organoid Seeding Medium at day 7 exhibit basal cell spheroid morphology. (B) Bright-field image of airway organoids differentiated in PneumaCult™ Airway Organoid Differentiation Medium at day 21 exhibit hollow lumens. (C) Airway organoid stained for ZO-1 (junction protein marker, red), MUC5AC (goblet cell marker, purple), AC-Tubulin (ciliated cell marker, green), and DAPI (nuclei, blue).

Comparison of Forskolin-treated airway organoids derived from healthy donors and CF donors.

Figure 3. Forskolin-Induced Swelling of Airway Organoids

(A) Forskolin-treated organoids derived from healthy donors increased in size compared to the DMSO control, indicating functional CFTR protein expression. (B) Forskolin-induced swelling is lost in organoids derived from CF donors, but re-established in VX-809-treated airway organoids. Error bars represent ± 95% confidence interval for the mean (n=3). Bright-field images of airway organoids taken during the Forskolin swelling assay at (C) 0 hours and (D) 6 hours show organoid swelling after treatment.

Ciliated cell percentage in fully differentiated airway organoids derived from healthy and cystic fibrosis (CF) donors using PneumaCult™ Airway Organoid Kit at different passages.

Figure 4. Fully Differentiated Airway Organoids Retain Morphological Characteristics at Different Passages

The ciliated cell percentage in organoids grown from (A) healthy and (B) CF donors using PneumaCult™ Airway Organoid Kit increased from P3 to P5. The total and ciliated cells were counted using a hemocytometer. Error bars represent ± 95% confidence interval for the mean (n=3).