若您需要咨询产品或有任何技术问题,请通过官方电话 400 885 9050 或邮箱 info.cn@stemcell.com 与我们联系

ArciTect™ 人 CRISPR 优化试剂盒

用于优化人细胞基因组编辑的完整试剂盒

产品号 #(选择产品)

产品号 #100-0470_C

用于优化人细胞基因组编辑的完整试剂盒

产品组分包括

  • ArciTect™人CRISPR优化试剂盒,APC(产品号 #100-0470)
    • ArciTect™人B2M sgRNA, 2 nmol 64.75µg;
    • ArciTect™Cas9核酸酶,100µg(产品号 #76002);
    • Anti-Human Beta-2 Microglobulin Antibody, Clone 35, APC,  25 tests;
  • ArciTect™ 人 CRISPR 优化试剂盒, PE (产品号 #100-0471)
    • ArciTect™ 人 B2M sgRNA, 2 nmol 64.75 µg
    • ArciTect™ Cas9核酸酶, 100 µg (产品号 #76002)
    • Anti-Human Beta-2 Microglobulin Antibody, Clone 35, PE, 25 tests
  • ArciTect™ 人 CRISPR 优化试剂盒, FITC (产品号 #100-0472)
    • ArciTect™ 人 B2M sgRNA, 2 nmol 64.75 µg
    • ArciTect™ Cas9核酸酶, 100 µg (产品号 #76002)
    • Anti-Human Beta-2 Microglobulin Antibody, Clone 35, FITC, 25 tests
专为您的实验方案打造的产品
要查看实验方案所需的所有配套产品,请参阅《实验方案与技术文档》

总览

ArciTect™ 人 CRISPR 优化试剂盒是一款基于流式细胞术的试剂盒,旨在快速优化人细胞中的基因组编辑。它也可用作在人细胞中使用 ArciTect™ CRISPR-Cas9 基因组编辑系统进行实验的阳性对照。该试剂盒包含 ArciTect™ 人 B2M sgRNA、ArciTect™ Cas9 核酸酶和荧光团偶联的 β-2 微球蛋白 (B2M) 抗体。ArciTect™ Cas9 核酸酶必须先与 ArciTect™ 人 B2M sgRNA 复合,然后递送至人细胞并培养 48 - 96 小时。培养后,可以通过收集并处理荧光团偶联的 B2M 抗体进行流式细胞术分析来快速评估编辑效率。

该试剂盒已通过测试和验证,可与 ArciTect™ 系列基因组编辑产品配合使用。通过靶向编码普遍表达的细胞表面蛋白的B2M基因,可以使用流式细胞术快速定量评估编辑效率。该技术还可以用于评估编辑成功率,例如活力监测和/或评估细胞类型特异性标记物的表达。

该试剂盒已通过测试和验证,可与 ArciTect™ 系列基因组编辑产品配合使用。通过靶向编码普遍表达的细胞表面蛋白的B2M基因,可以使用流式细胞术快速定量评估编辑效率。该技术还可以用于评估编辑成功率,例如活力监测和/或评估细胞类型特异性标记物的表达。

该试剂盒已通过测试和验证,可与 ArciTect™ 系列基因组编辑产品配合使用。通过靶向编码普遍表达的细胞表面蛋白的B2M基因,可以使用流式细胞术快速定量评估编辑效率。该技术还可以用于评估编辑成功率,例如活力监测和/或评估细胞类型特异性标记物的表达。

该试剂盒已通过测试和验证,可与 ArciTect™ 系列基因组编辑产品配合使用。通过靶向编码普遍表达的细胞表面蛋白的B2M基因,可以使用流式细胞术快速定量评估编辑效率。该技术还可以用于评估编辑成功率,例如活力监测和/或评估细胞类型特异性标记物的表达。

细胞类型
造血干/祖细胞,肠道细胞,多能干细胞,T 细胞
 
种属

 
应用
基因组编辑
 
研究领域
免疫,类器官,干细胞生物学
 

实验数据

Figure 1. ArciTect™ Human CRISPR Optimization Kit Exhibits High Editing Efficiency in hPSCs, T Cells, and CD34+ Hematopoietic Stem and Progenitor Cells (HSPCs)

Cells were electroporated with RNP complexes containing sgRNA targeting B2M complexed with ArciTect™ Cas9 Nuclease protein. The cells were cultured for 72 hours following electroporation and editing efficiency was monitored by flow cytometry to detect loss of B2M expression; n = 3 biological replicates (WLS-1C iPSCs/hPSCs) or n = 3 donors (T cells and CD34+ HSPCs). No EP: non-electroporated controls.

Figure 2. Anti-Human Beta-2-Microglobulin, Clone #35 Conjugates Exhibit Equivalent Performance Across Samples to Monitor B2M Knockout Efficiency Using the ArciTect™ Human CRISPR Optimization Kit

CD34+ HSPCs isolated from two independent donors were electroporated with RNP complexes containing sgRNA targeting B2M complexed with ArciTect™ Cas9 Nuclease protein. The cells were cultured for 96 hours following electroporation and editing efficiency was monitored by flow cytometry to detect loss of B2M expression using anti-human beta-2-microglobulin, clone #35, (A, B) APC-conjugated, (C, D) FITC-conjugated, and (E, F) PE-conjugated. The histograms were derived from gated events of viable (7-AAD-negative) cells. Orange histograms: RNP-electroporated samples; grey histograms: non-electroporated control samples.

产品说明书及文档

请在《产品说明书》中查找相关支持信息和使用说明,或浏览下方更多实验方案。

Document Type
Product Name
Catalog #
Lot #
Language
Catalog #
100-0470, 100-0472, 100-0471
Lot #
All
Language
English
Document Type
Safety Data Sheet 3
Catalog #
100-0470
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Catalog #
100-0470, 100-0472, 100-0471
Lot #
All
Language
English
Document Type
Safety Data Sheet 3
Catalog #
100-0470, 100-0472, 100-0471
Lot #
All
Language
English
Document Type
Safety Data Sheet 3
Catalog #
100-0472
Lot #
All
Language
English
Document Type
Safety Data Sheet 3
Catalog #
100-0471
Lot #
All
Language
English

更多信息

更多信息
种属 Human
法律声明:

The purchase of the ArciTect™ products conveys to the purchaser the limited, non-transferable right to use, in accordance with all applicable laws and regulations, the ArciTect™ products and any related material solely for research purposes only, not for any clinical, human, agricultural, veterinary, livestock, or commercial purpose, with no warranty (express or implied) from the owners or assignees of the Patents or STEMCELL Technologies each of whom expressly disclaim any warranty regarding results obtained through the use of the ArciTect™ products, and without any exception the owners or assignees of the Patents or STEMCELL Technologies, or as applicable a director, trustee, officer, employee, agent, faculty, official investigator or student thereof, will not suffer or be exposed to any liability, damages, loss, or expense of any kind whatsoever arising from or related to the use of the ArciTect™ products by a purchaser of same. Distribution of ArciTect™ products by STEMCELL Technologies is covered under at least US 8,697,359, US 8,771,945, US 8,795,965, US 8,865,406, US 8,871,445, US 8,889,356, US 8,889,418, US 8,895,308, US 8,906,616 and foreign equivalents (“Patents”). 质量保证:

产品仅供研究使用,不用于针对人或动物的诊断或治疗。 欲获悉更多关于STEMCELL的质控信息,请访问 STEMCELL.CN/COMPLIANCE.
Copyright © 2025 by STEMCELL Technologies. All rights reserved.