EasySep™小鼠TIL（CD45）正选试剂盒
产品号 #08570_C
用于人脑类器官建立与成熟的培养基试剂盒
若您需要咨询产品或有任何技术问题，请通过官方电话 400 885 9050 或邮箱 info.cn@stemcell.com 与我们联系
Need a high-quality cell source? Use the hiPSC SCTi003-A (female) control line, manufactured with mTeSR™ Plus.
专为您的实验方案打造的产品
要查看实验方案所需的所有配套产品，请参阅《实验方案与技术文档》。
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Ultra-Low Adherent Plate for Suspension Cultu...Sterile, flat-bottom plate, with ultra-low adherent surface for suspension cultures, with lid; 6-well format
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Y-27632 (Dihydrochloride)RHO/ROCK pathway inhibitor; Inhibits ROCK1 and ROCK2
What Our Scientist Says
Human brain development is complex, so to observe it in a dish is a huge scientific breakthrough. We've tried to simplify that process and make it more accessible to you, regardless of how much stem cell experience you have.
Leon ChewScientist
总览
生成自组织的、由多能干细胞 (PSC) 衍生的神经类器官，其细胞组成和结构组织与发育中的人脑相似。
这些成分明确的无血清细胞培养基和简单的四阶段实验方案基于 Lancaster 等人发表的配方（Lancaster MA 等人，Nature，2013 和 Lancaster MA 等人，Science，2014），旨在更可靠地生成脑类器官。使用 STEMdiff™ 脑类器官试剂盒生成的类器官从胚状体 (EB) 形成步骤开始，随后神经上皮细胞扩增，具有皮质样区域，包括脑室区 (PAX6+/SOX2+/Ki-67+)、外脑室下区 (Ki-67+/p-Vimentin+)、中间区 (TBR2+) 和皮质板 (CTIP2+/MAP2+/TBR1+)，其分层方向与体内观察到的相似。
对于长期培养（> 40 天），成熟所需的成分可作为STEMdiff™ 脑类器官成熟试剂盒购买。
这些成分明确的无血清细胞培养基和简单的四阶段实验方案基于 Lancaster 等人发表的配方（Lancaster MA 等人，Nature，2013 和 Lancaster MA 等人，Science，2014），旨在更可靠地生成脑类器官。使用 STEMdiff™ 脑类器官试剂盒生成的类器官从胚状体 (EB) 形成步骤开始，随后神经上皮细胞扩增，具有皮质样区域，包括脑室区 (PAX6+/SOX2+/Ki-67+)、外脑室下区 (Ki-67+/p-Vimentin+)、中间区 (TBR2+) 和皮质板 (CTIP2+/MAP2+/TBR1+)，其分层方向与体内观察到的相似。
对于长期培养（> 40 天），成熟所需的成分可作为STEMdiff™ 脑类器官成熟试剂盒购买。
这些成分明确的无血清细胞培养基和简单的四阶段实验方案基于 Lancaster 等人发表的配方（Lancaster MA 等人，Nature，2013 和 Lancaster MA 等人，Science，2014），旨在更可靠地生成脑类器官。使用 STEMdiff™ 脑类器官试剂盒生成的类器官从胚状体 (EB) 形成步骤开始，随后神经上皮细胞扩增，具有皮质样区域，包括脑室区 (PAX6+/SOX2+/Ki-67+)、外脑室下区 (Ki-67+/p-Vimentin+)、中间区 (TBR2+) 和皮质板 (CTIP2+/MAP2+/TBR1+)，其分层方向与体内观察到的相似。
对于长期培养（> 40 天），成熟所需的成分可作为STEMdiff™ 脑类器官成熟试剂盒购买。
这些成分明确的无血清细胞培养基和简单的四阶段实验方案基于 Lancaster 等人发表的配方（Lancaster MA 等人，Nature，2013 和 Lancaster MA 等人，Science，2014），旨在更可靠地生成脑类器官。使用 STEMdiff™ 脑类器官试剂盒生成的类器官从胚状体 (EB) 形成步骤开始，随后神经上皮细胞扩增，具有皮质样区域，包括脑室区 (PAX6+/SOX2+/Ki-67+)、外脑室下区 (Ki-67+/p-Vimentin+)、中间区 (TBR2+) 和皮质板 (CTIP2+/MAP2+/TBR1+)，其分层方向与体内观察到的相似。
对于长期培养（> 40 天），成熟所需的成分可作为STEMdiff™ 脑类器官成熟试剂盒购买。
亚型
专用培养基
细胞类型
神经细胞，PSC衍生，神经干/祖细胞，多能干细胞
种属
人
应用
细胞培养，鉴定，分化，功能学筛选，免疫荧光，类器官培养，表型鉴定，球状体培养
品牌
STEMdiff
研究领域
疾病建模，药物发现和毒理检测，神经科学，干细胞生物学
制剂类别
无血清
实验数据
产品说明书及文档
请在《产品说明书》中查找相关支持信息和使用说明，或浏览下方更多实验方案。
应用领域
本产品专为以下研究领域设计，适用于工作流程中的高亮阶段。探索这些工作流程，了解更多我们为各研究领域提供的其他配套产品。
相关材料与文献
技术资料 (46)
文献 (5)
Human CNS barrier-forming organoids with cerebrospinal fluid production. Science (New York, N.Y.) 2020
Abstract
Cerebrospinal fluid (CSF) is a vital liquid, providing nutrients and signaling molecules and clearing out toxic by-products from the brain. The CSF is produced by the choroid plexus (ChP), a protective epithelial barrier that also prevents free entry of toxic molecules or drugs from the blood. Here, we establish human ChP organoids with a selective barrier and CSF-like fluid secretion in self-contained compartments. We show that this in vitro barrier exhibits the same selectivity to small molecules as the ChP in vivo and that ChP-CSF organoids can predict central nervous system (CNS) permeability of new compounds. The transcriptomic and proteomic signatures of ChP-CSF organoids reveal a high degree of similarity to the ChP in vivo. Finally, the intersection of single-cell transcriptomics and proteomic analysis uncovers key human CSF components produced by previously unidentified specialized epithelial subtypes.
One-Stop Microfluidic Assembly of Human Brain Organoids To Model Prenatal Cannabis Exposure. Analytical chemistry 2020
Abstract
Prenatal cannabis exposure (PCE) influences human brain development, but it is challenging to model PCE using animals and current cell culture techniques. Here, we developed a one-stop microfluidic platform to assemble and culture human cerebral organoids from human embryonic stem cells (hESC) to investigate the effect of PCE on early human brain development. By incorporating perfusable culture chambers, air-liquid interface, and one-stop protocol, this microfluidic platform can simplify the fabrication procedure and produce a large number of organoids (169 organoids per 3.5 cm × 3.5 cm device area) without fusion, as compared with conventional fabrication methods. These one-stop microfluidic assembled cerebral organoids not only recapitulate early human brain structure, biology, and electrophysiology but also have minimal size variation and hypoxia. Under on-chip exposure to the psychoactive cannabinoid, $\Delta$-9-tetrahydrocannabinol (THC), cerebral organoids exhibited reduced neuronal maturation, downregulation of cannabinoid receptor type 1 (CB1) receptors, and impaired neurite outgrowth. Moreover, transient on-chip THC treatment also decreased spontaneous firing in these organoids. This one-stop microfluidic technique enables a simple, scalable, and repeatable organoid culture method that can be used not only for human brain organoids but also for many other human organoids including liver, kidney, retina, and tumor organoids. This technology could be widely used in modeling brain and other organ development, developmental disorders, developmental pharmacology and toxicology, and drug screening.
Cerebral organoids at the air–liquid interface generate diverse nerve tracts with functional output Nature Neuroscience 2019 apr
Abstract
Neural organoids have the potential to improve our understanding of human brain development and neurological disorders. However, it remains to be seen whether these tissues can model circuit formation with functional neuronal output. Here we have adapted air–liquid interface culture to cerebral organoids, leading to improved neuronal survival and axon outgrowth. The resulting thick axon tracts display various morphologies, including long-range projection within and away from the organoid, growth-cone turning, and decussation. Single-cell RNA sequencing reveals various cortical neuronal identities, and retrograde tracing demonstrates tract morphologies that match proper molecular identities. These cultures exhibit active neuronal networks, and subcortical projecting tracts can innervate mouse spinal cord explants and evoke contractions of adjacent muscle in a manner dependent on intact organoid-derived innervating tracts. Overall, these results reveal a remarkable self-organization of corticofugal and callosal tracts with a functional output, providing new opportunities to examine relevant aspects of human CNS development and disease.
更多信息
| 种属 | Human |
|---|---|
| 配方类别 | Serum-Free |
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法律声明:
This product was developed under a license to intellectual property owned by the Institute of Molecular Biotechnology (IMBA) of the Austrian Academy of Sciences. This product is sold for research use only (whether the buyer is an academic or for-profit entity) under a non-transferable, limited-use license. Purchase of this product does not include the right to sell, use or otherwise transfer this product for commercial purposes (i.e., any activity undertaken for consideration, such as use of this product for manufacturing, or resale of this product or any materials made using this product, or use of this product or any materials made using this product to provide services or, in collaboration with, a for-profit entity, for purposes other than research applications (i.e., drug development activities). Purchasers wishing to use the product for commercial purposes should contact IMBA at technologvtransfer@imba.oeaw.ac.at. 质量保证:
产品仅供研究使用，不用于针对人或动物的诊断或治疗。 欲获悉更多关于STEMCELL的质控信息，请访问 STEMCELL.CN/COMPLIANCE.
This product was developed under a license to intellectual property owned by the Institute of Molecular Biotechnology (IMBA) of the Austrian Academy of Sciences. This product is sold for research use only (whether the buyer is an academic or for-profit entity) under a non-transferable, limited-use license. Purchase of this product does not include the right to sell, use or otherwise transfer this product for commercial purposes (i.e., any activity undertaken for consideration, such as use of this product for manufacturing, or resale of this product or any materials made using this product, or use of this product or any materials made using this product to provide services or, in collaboration with, a for-profit entity, for purposes other than research applications (i.e., drug development activities). Purchasers wishing to use the product for commercial purposes should contact IMBA at technologvtransfer@imba.oeaw.ac.at. 质量保证:
产品仅供研究使用，不用于针对人或动物的诊断或治疗。 欲获悉更多关于STEMCELL的质控信息，请访问 STEMCELL.CN/COMPLIANCE.
