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EasySep™ Release小鼠PE正选试剂盒

采用可解离磁珠技术对PE偶联抗体标记的小鼠的细胞进行免疫磁珠正选
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产品号 #（选择产品）

产品号 #17656_C

免选磁珠正选出不含磁珠的PE抗体标记小鼠细胞

产品优势

  • 只需不到40分钟，从小鼠组织中分离出用PE抗体标记的高纯度细胞
  • 无需洗涤去除EasySep™ Releasable RapidSpheres™可解离磁珠

产品组分包括

  • EasySep™ Release小鼠PE正选试剂盒（产品号 #17656）
  • EasySep™PE正选抗体混合物，1 mL
  • EasySep™ Releasable RapidSpheres™ 50201磁珠，1 mL
  • EasySep™Release缓冲液（浓缩），3 x 1 mL
  • EasySep™小鼠FcR阻断剂（产品号#18731），0.5 mL
main product photo
New format, same high quality! You may notice that your kit contents and packaging look slightly different from previous orders. We are currently updating the format of select EasySep™ Mouse kits to include a Mouse FcR blocker instead of Normal Rat Serum. With this change, all components will now be shipped in a single package, while providing the same cell isolation performance as before.
专为您的实验方案打造的产品
要查看实验方案所需的所有配套产品，请参阅《实验方案与技术文档》。
  1. EasySep™ Magnet
    EasySep™ Magnet

    Magnet for column-free immunomagnetic separation

  2. EasySep™ Buffer
    EasySep™ Buffer

    Cell separation buffer

总览
实验数据
产品说明书及文档
相关材料与文献
相关产品

总览

使用 EasySep™ Release小鼠PE正选试剂盒，可通过免疫磁性正选，轻松从小鼠脾细胞中分离出高纯度、且不含磁珠的PE抗体标记的小鼠细胞。EasySep™技术结合单克隆抗体的特异性和无柱磁分选系统的简便性，已在发表的研究中广泛应用超过20年。

该EasySep™阳性分选流程中，首先用能识别PE和磁珠（称为EasySep™可解离磁珠）的抗体复合物标记目标细胞。与传统磁珠结合目标细胞不同，这些磁珠具有可解离的特性。经EasySep™磁极分选后，目的细胞首先被抗体和特异的磁珠标记，非目的细胞通过简单倾倒弃去，而目的细胞则保留在管中。随后，使用解离试剂将结合的磁珠从经EasySep™分选、带有PE抗体标记的细胞上解离。仅需不到40分钟完成分选，目的细胞可立即用于流式细胞术、细胞培养或DNA/RNA提取等下游应用。使用该EasySep™试剂盒分选之后，细胞表面仍结合有抗体复合物，并可能与Brilliant Violet™偶联的抗体、聚乙二醇修饰的蛋白质或其他化学相关配体相互作用。 

了解更多关于免疫磁珠EasySep™技术的工作原理。探索更多为您的实验流程优化的产品，包括培养基、添加剂、抗体等配套试剂。

该EasySep™阳性分选流程中，首先用能识别PE和磁珠（称为EasySep™可解离磁珠）的抗体复合物标记目标细胞。与传统磁珠结合目标细胞不同，这些磁珠具有可解离的特性。经EasySep™磁极分选后，目的细胞首先被抗体和特异的磁珠标记，非目的细胞通过简单倾倒弃去，而目的细胞则保留在管中。随后，使用解离试剂将结合的磁珠从经EasySep™分选、带有PE抗体标记的细胞上解离。仅需不到40分钟完成分选，目的细胞可立即用于流式细胞术、细胞培养或DNA/RNA提取等下游应用。使用该EasySep™试剂盒分选之后，细胞表面仍结合有抗体复合物，并可能与Brilliant Violet™偶联的抗体、聚乙二醇修饰的蛋白质或其他化学相关配体相互作用。 

了解更多关于免疫磁珠EasySep™技术的工作原理。探索更多为您的实验流程优化的产品，包括培养基、添加剂、抗体等配套试剂。

该EasySep™阳性分选流程中，首先用能识别PE和磁珠（称为EasySep™可解离磁珠）的抗体复合物标记目标细胞。与传统磁珠结合目标细胞不同，这些磁珠具有可解离的特性。经EasySep™磁极分选后，目的细胞首先被抗体和特异的磁珠标记，非目的细胞通过简单倾倒弃去，而目的细胞则保留在管中。随后，使用解离试剂将结合的磁珠从经EasySep™分选、带有PE抗体标记的细胞上解离。仅需不到40分钟完成分选，目的细胞可立即用于流式细胞术、细胞培养或DNA/RNA提取等下游应用。使用该EasySep™试剂盒分选之后，细胞表面仍结合有抗体复合物，并可能与Brilliant Violet™偶联的抗体、聚乙二醇修饰的蛋白质或其他化学相关配体相互作用。 

了解更多关于免疫磁珠EasySep™技术的工作原理。探索更多为您的实验流程优化的产品，包括培养基、添加剂、抗体等配套试剂。

该EasySep™阳性分选流程中，首先用能识别生物素和磁珠（称为EasySep™可解离磁珠）的抗体复合物标记目标细胞。与传统磁珠结合目标细胞不同，这些磁珠具有可解离的特性。经EasySep™磁极分选后，目的细胞首先被抗体和特异的磁珠标记，非目的细胞通过简单倾倒弃去，而目的细胞则保留在管中。随后，使用解离试剂将结合的磁珠从经EasySep™ 分选、带有生物素抗体标记的细胞上解离。仅需不到40分钟完成分选，目的细胞可立即用于流式细胞术、细胞培养或DNA/RNA提取等下游应用。使用该EasySep™试剂盒分选之后，细胞表面仍结合有抗体复合物，并可能与Brilliant Violet™偶联的抗体、聚乙二醇修饰的蛋白质或其他化学相关配体相互作用。 

了解更多关于免疫磁珠EasySep™技术的工作原理。探索更多为您的实验流程优化的产品，包括培养基、添加剂、抗体等配套试剂。

磁体兼容性
• EasySep™ Magnet (Catalog #18000) • “The Big Easy” EasySep™ Magnet (Catalog #18001) • EasyPlate™ Magnet (Catalog #18102) • EasyEights™ Magnet (Catalog #18103)
 
亚型
细胞分选试剂盒
 
细胞类型
B 细胞，树突状细胞（DCs），粒细胞及其亚群，造血干/祖细胞，巨噬细胞，骨髓基质细胞，间充质干/祖细胞，单核细胞，单个核细胞，髓系细胞，NK 细胞，其它细胞系，血浆，T 细胞
 
样本来源
Bone Marrow，其它细胞系，Spleen
 
筛选方法
Positive
 
应用
细胞分选
 
品牌
EasySep
 
研究领域
免疫
 

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实验数据

Typical EasySep™ Release Mouse PE Positive Selection Kit Cell Isolation Profile

Figure 1. Typical EasySep™ Release Mouse PE Positive Selection Kit Cell Isolation Profile

Starting with mouse splenocytes, the purities of the start and final isolated fractions are 59.1% and 98.6%, respectively, using a PE-conjugated anti-mouse CD19 antibody and EasySep™ Release Mouse Positive Selection Kit.

Typical Cell Purity Following Mouse Lung Epithelial Cell Isolation Using the EasySep™ Release Positive Selection Kit

Figure 2. Typical Cell Purity Following Mouse Lung Epithelial Cell Isolation Using the EasySep™ Release Positive Selection Kit

Mouse lung cells were firstly labeled with PE-conjugated anti-mouse CD326 (EpCAM) and biotin-conjugated CD45 antibodies. EpCAM+ cells were positively selected using EasySep™ Release Mouse PE Positive Selection Kit (Catalog #17656), and subsequently, CD45+ contaminating leukocytes were depleted using EasySep™ Mouse Biotin Positive Selection Kit (Catalog #17655). Isolated EpCAM+CD45- cells were 86% pure.

产品说明书及文档

请在《产品说明书》中查找相关支持信息和使用说明，或浏览下方更多实验方案。

Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet
Product Name
EasySep™ Release Mouse PE Positive Selection Kit
Catalog #
17656
Lot #
1000159295 or higher
Language
English
Document Type
Safety Data Sheet 1
Product Name
EasySep™ Release Mouse PE Positive Selection Kit
Catalog #
17656
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Product Name
EasySep™ Release Mouse PE Positive Selection Kit
Catalog #
17656
Lot #
All
Language
English
Document Type
Safety Data Sheet 3
Product Name
EasySep™ Release Mouse PE Positive Selection Kit
Catalog #
17656
Lot #
All
Language
English
Document Type
Safety Data Sheet 4
Product Name
EasySep™ Release Mouse PE Positive Selection Kit
Catalog #
17656
Lot #
All
Language
English
Document Type
Safety Data Sheet 5
Product Name
EasySep™ Release Mouse PE Positive Selection Kit
Catalog #
17656
Lot #
All
Language
English

相关材料与文献

技术资料 (9)

Tools for Your Immunology Research
Brochure
Tools for Your Immunology Research
EasySep™ Cell Separation Technology
Brochure
EasySep™ Cell Separation Technology
EasySep™ Release Free Your Positively Selected Cells from Magnetic Particles
Brochure
EasySep™ Release Free Your Positively Selected Cells from Magnetic Particles
Isolate Mouse CD45.1 or CD45.2 Positive Cells with EasySep™ Release Mouse Positive Selection Kits
Technical Bulletin
Isolate Mouse CD45.1 or CD45.2 Positive Cells with EasySep™ Release Mouse Positive Selection Kits
Human Immune Cytokines
Wallchart
Human Immune Cytokines
Frequencies and Percentages of Mouse Immune Cell Types
Wallchart
Frequencies and Percentages of Mouse Immune Cell Types
How EasySep™ Magnetic Cell Separation Technology Works: Fast and Easy Cell Isolation
1:57
Video
How EasySep™ Magnetic Cell Separation Technology Works: Fast and Easy Cell Isolation
Isolate Cells with a Simple Pour-Off: EasySep™ Cell Separation Technology
1:13
Video
Isolate Cells with a Simple Pour-Off: EasySep™ Cell Separation Technology
Positive Selection of PE- or Biotin-Conjugated Antibody Labeled Cells with Releasable Rapidspheres™
Scientific Poster
Positive Selection of PE- or Biotin-Conjugated Antibody Labeled Cells with Releasable Rapidspheres�...

文献 (2)

Accumulation of ?? T cells in visceral fat with aging promotes chronic inflammation. M. E. C. Bruno et al. GeroScience 2022 jun

Abstract

Adipose tissue dysfunction is strongly linked to the development of chronic inflammation and cardiometabolic disorders in aging. While much attention has been given to the role of resident adipose tissue immune cells in the disruption of homeostasis in obesity, age-specific effects remain understudied. Here, we identified and characterized a population of ?? T cells, which show unique age-dependent accumulation in the visceral adipose tissue (VAT) of both mice and humans. Diet-induced obesity likewise increased ?? T cell numbers; however, the effect was greater in the aged where the increase was independent of fat mass. ?? T cells in VAT express a tissue-resident memory T cell phenotype (CD44hiCD62LlowCD69+) and are predominantly IL-17A-producing cells. Transcriptome analyses of immunomagnetically purified ?? T cells identified significant age-associated differences in expression of genes related to inflammation, immune cell composition, and adipocyte differentiation, suggesting age-dependent qualitative changes in addition to the quantitative increase. Genetic deficiency of ?? T cells in old age improved the metabolic phenotype, characterized by increased respiratory exchange ratio, and lowered levels of IL-6 both systemically and locally in VAT. Decreased IL-6 was predominantly due to reduced production by non-immune stromal cells, primarily preadipocytes, and adipose-derived stem cells. Collectively, these findings suggest that an age-dependent increase of tissue-resident ?? T cells in VAT contributes to local and systemic chronic inflammation and metabolic dysfunction in aging.
View publication
Enzymatic Preparation of 2'-5',3'-5'-Cyclic Dinucleotides, Their Binding Properties to Stimulator of Interferon Genes Adaptor Protein, and Structure/Activity Correlations. B. Novotn\'a et al. Journal of medicinal chemistry 2019 dec

Abstract

Cyclic dinucleotides are second messengers in the cyclic GMP-AMP synthase (cGAS)-stimulator of interferon genes (STING) pathway, which plays an important role in recognizing tumor cells and viral or bacterial infections. They bind to the STING adaptor protein and trigger expression of cytokines via TANK binding kinase 1 (TBK1)/interferon regulatory factor 3 (IRF3) and inhibitor of nuclear factor-$\kappa$B (I$\kappa$B) kinase (IKK)/nuclear factor-$\kappa$B (NF$\kappa$B) signaling cascades. In this work, we describe an enzymatic preparation of 2'-5',3'-5'-cyclic dinucleotides (2'3'CDNs) with use of cyclic GMP-AMP synthases (cGAS) from human, mouse, and chicken. We profile substrate specificity of these enzymes by employing a small library of nucleotide-5'-triphosphate (NTP) analogues and use them to prepare 33 2'3'CDNs. We also determine affinity of these CDNs to five different STING haplotypes in cell-based and biochemical assays and describe properties needed for their optimal activity toward all STING haplotypes. Next, we study their effect on cytokine and chemokine induction by human peripheral blood mononuclear cells (PBMCs) and evaluate their cytotoxic effect on monocytes. Additionally, we report X-ray crystal structures of two new CDNs bound to STING protein and discuss structure-activity relationship by using quantum and molecular mechanical (QM/MM) computational modeling.
View publication
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更多信息

更多信息
Magnet Compatibility • EasySep™ Magnet (Catalog #18000) • “The Big Easy” EasySep™ Magnet (Catalog #18001) • EasyPlate™ Magnet (Catalog #18102) • EasyEights™ Magnet (Catalog #18103)
样本来源 Bone Marrow, Other, Spleen
Selection Method Positive
质量保证:

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