切换导航
  • 比较产品
我的购物车

若您需要咨询产品或有任何技术问题，请通过官方电话 400 885 9050 或邮箱 info.cn@stemcell.com 与我们联系

EasySep™ Release小鼠Biotin正选试剂盒

采用可解离磁珠技术对生物素偶联抗体标记的小鼠的细胞进行免疫磁珠正选
率先评论此产品
添加并比较

产品号 #（选择产品）

产品号 #17655_C

免选磁珠正选出不含磁珠的生物素化抗体标记小鼠细胞

产品优势

  • 只需不到40分钟，从小鼠组织中分离出用生物素化抗体标记的高纯度细胞
  • 无需洗涤去除EasySep™ Releasable RapidSpheres™可解离磁珠

产品组分包括

  • EasySep™ Release小鼠Biotin正选试剂盒（产品号 #17655）
    • EasySep™Biotin正选抗体混合物，0.5 mL
    • EasySep™ Releasable RapidSpheres™ 50201磁珠，1 mL
    • EasySep™Release缓冲液（浓缩），3 x 1 mL
  • EasySep™小鼠FcR阻断剂（产品号#18731），0.5 mL
main product photo
New format, same high quality! You may notice that your kit contents and packaging look slightly different from previous orders. We are currently updating the format of select EasySep™ Mouse kits to include a Mouse FcR blocker instead of Normal Rat Serum. With this change, all components will now be shipped in a single package, while providing the same cell isolation performance as before.
专为您的实验方案打造的产品
要查看实验方案所需的所有配套产品，请参阅《实验方案与技术文档》。
  1. EasySep™ Magnet
    EasySep™ Magnet

    Magnet for column-free immunomagnetic separation

  2. EasySep™ Buffer
    EasySep™ Buffer

    Cell separation buffer

总览
实验数据
产品说明书及文档
相关材料与文献
相关产品

总览

使用 EasySep™ Release小鼠Biotin正选试剂盒，可通过免疫磁性正选，轻松从小鼠脾细胞中分离出高纯度、且不含磁珠的生物素化抗体标记的小鼠细胞。EasySep™技术结合单克隆抗体的特异性和无柱磁分选系统的简便性，已在发表的研究中广泛应用超过20年。

该EasySep™阳性分选流程中，首先用能识别生物素和磁珠（称为EasySep™可解离磁珠）的抗体复合物标记目标细胞。与传统磁珠结合目标细胞不同，这些磁珠具有可解离的特性。经EasySep™磁极分选后，目的细胞首先被抗体和特异的磁珠标记，非目的细胞通过简单倾倒弃去，而目的细胞则保留在管中。随后，使用解离试剂将结合的磁珠从经EasySep™ 分选、带有生物素抗体标记的细胞上解离。仅需不到40分钟完成分选，目的细胞可立即用于流式细胞术、细胞培养或DNA/RNA提取等下游应用。使用该EasySep™试剂盒分选之后，细胞表面仍结合有抗体复合物，并可能与Brilliant Violet™偶联的抗体、聚乙二醇修饰的蛋白质或其他化学相关配体相互作用。 

了解更多关于免疫磁珠EasySep™技术的工作原理。探索更多为您的实验流程优化的产品，包括培养基、添加剂、抗体等配套试剂。

该EasySep™阳性分选流程中，首先用能识别生物素和磁珠（称为EasySep™可解离磁珠）的抗体复合物标记目标细胞。与传统磁珠结合目标细胞不同，这些磁珠具有可解离的特性。经EasySep™磁极分选后，目的细胞首先被抗体和特异的磁珠标记，非目的细胞通过简单倾倒弃去，而目的细胞则保留在管中。随后，使用解离试剂将结合的磁珠从经EasySep™ 分选、带有生物素抗体标记的细胞上解离。仅需不到40分钟完成分选，目的细胞可立即用于流式细胞术、细胞培养或DNA/RNA提取等下游应用。使用该EasySep™试剂盒分选之后，细胞表面仍结合有抗体复合物，并可能与Brilliant Violet™偶联的抗体、聚乙二醇修饰的蛋白质或其他化学相关配体相互作用。 

了解更多关于免疫磁珠EasySep™技术的工作原理。探索更多为您的实验流程优化的产品，包括培养基、添加剂、抗体等配套试剂。

该EasySep™阳性分选流程中，首先用能识别生物素和磁珠（称为EasySep™可解离磁珠）的抗体复合物标记目标细胞。与传统磁珠结合目标细胞不同，这些磁珠具有可解离的特性。经EasySep™磁极分选后，目的细胞首先被抗体和特异的磁珠标记，非目的细胞通过简单倾倒弃去，而目的细胞则保留在管中。随后，使用解离试剂将结合的磁珠从经EasySep™ 分选、带有生物素抗体标记的细胞上解离。仅需不到40分钟完成分选，目的细胞可立即用于流式细胞术、细胞培养或DNA/RNA提取等下游应用。使用该EasySep™试剂盒分选之后，细胞表面仍结合有抗体复合物，并可能与Brilliant Violet™偶联的抗体、聚乙二醇修饰的蛋白质或其他化学相关配体相互作用。 

了解更多关于免疫磁珠EasySep™技术的工作原理。探索更多为您的实验流程优化的产品，包括培养基、添加剂、抗体等配套试剂。

该EasySep™阳性分选流程中，首先用能识别生物素和磁珠（称为EasySep™可解离磁珠）的抗体复合物标记目标细胞。与传统磁珠结合目标细胞不同，这些磁珠具有可解离的特性。经EasySep™磁极分选后，目的细胞首先被抗体和特异的磁珠标记，非目的细胞通过简单倾倒弃去，而目的细胞则保留在管中。随后，使用解离试剂将结合的磁珠从经EasySep™ 分选、带有生物素抗体标记的细胞上解离。仅需不到40分钟完成分选，目的细胞可立即用于流式细胞术、细胞培养或DNA/RNA提取等下游应用。使用该EasySep™试剂盒分选之后，细胞表面仍结合有抗体复合物，并可能与Brilliant Violet™偶联的抗体、聚乙二醇修饰的蛋白质或其他化学相关配体相互作用。

磁体兼容性
• EasySep™ Magnet (Catalog #18000) • “The Big Easy” EasySep™ Magnet (Catalog #18001) • EasyPlate™ Magnet (Catalog #18102) • EasyEights™ Magnet (Catalog #18103)
 
亚型
细胞分选试剂盒
 
细胞类型
B 细胞，树突状细胞（DCs），粒细胞及其亚群，造血干/祖细胞，先天性淋巴细胞，淋巴细胞，巨噬细胞，骨髓基质细胞，间充质干/祖细胞，单核细胞，单个核细胞，髓系细胞，NK 细胞，其它细胞系，血浆，T 细胞
 
种属
小鼠
 
样本来源
Bone Marrow，其它细胞系，Spleen
 
筛选方法
Positive
 
应用
细胞分选
 
品牌
EasySep
 
研究领域
免疫
 

查看更多 显示更少

实验数据

Typical EasySep™ Release Mouse Biotin Positive Selection Kit Cell Isolation Profile from PBMCs

Figure 1. Typical EasySep™ Release Mouse Biotin Positive Selection Kit Cell Isolation Profile from PBMCs

Starting with fresh PBMCs, the purities of the start and final isolated fractions are 34.6% and 97.1%, respectively, using a biotinylated anti-human CD45RO antibody and EasySep™ Release Human Biotin Positive Selection Kit (as assessed by labeling with CD45RO and CD45RA).

Typical EasySep™ Release Mouse Biotin Positive Selection Kit Cell Isolation Profile from Splenocytes

Figure 2. Typical EasySep™ Release Mouse Biotin Positive Selection Kit Cell Isolation Profile from Splenocytes

Starting with mouse splenocytes, the purities of the start and final isolated fractions are 58.4% and 95.1%, respectively, using a biotinylated anti-mouse CD19 antibody and EasySep™ Release Mouse Biotin Positive Selection Kit (as assessed by labeling with CD19 and CD45R/B220).

产品说明书及文档

请在《产品说明书》中查找相关支持信息和使用说明，或浏览下方更多实验方案。

Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet 1
Product Name
EasySep™ Release Mouse Biotin Positive Selection Kit
Catalog #
17655
Lot #
1000157902 or higher
Language
English
Document Type
Product Information Sheet 2
Product Name
EasySep™ Release Mouse Biotin Positive Selection Kit
Catalog #
17655
Lot #
1000157901 or lower
Language
English
Document Type
Safety Data Sheet 1
Product Name
EasySep™ Release Mouse Biotin Positive Selection Kit
Catalog #
17655
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Product Name
EasySep™ Release Mouse Biotin Positive Selection Kit
Catalog #
17655
Lot #
All
Language
English
Document Type
Safety Data Sheet 3
Product Name
EasySep™ Release Mouse Biotin Positive Selection Kit
Catalog #
17655
Lot #
All
Language
English
Document Type
Safety Data Sheet 4
Product Name
EasySep™ Release Mouse Biotin Positive Selection Kit
Catalog #
17655
Lot #
All
Language
English
Document Type
Safety Data Sheet 5
Product Name
EasySep™ Release Mouse Biotin Positive Selection Kit
Catalog #
17655
Lot #
All
Language
English

相关材料与文献

技术资料 (8)

EasySep™ Cell Separation Technology
Brochure
EasySep™ Cell Separation Technology
EasySep™ Release Free Your Positively Selected Cells from Magnetic Particles
Brochure
EasySep™ Release Free Your Positively Selected Cells from Magnetic Particles
Tools for Your Immunology Research
Brochure
Tools for Your Immunology Research
Isolate Mouse CD45.1 or CD45.2 Positive Cells with EasySep™ Release Mouse Positive Selection Kits
Technical Bulletin
Isolate Mouse CD45.1 or CD45.2 Positive Cells with EasySep™ Release Mouse Positive Selection Kits
Frequencies and Percentages of Mouse Immune Cell Types
Wallchart
Frequencies and Percentages of Mouse Immune Cell Types
Human Immune Cytokines
Wallchart
Human Immune Cytokines
How EasySep™ Magnetic Cell Separation Technology Works: Fast and Easy Cell Isolation
1:57
Video
How EasySep™ Magnetic Cell Separation Technology Works: Fast and Easy Cell Isolation
Positive Selection of PE- or Biotin-Conjugated Antibody Labeled Cells with Releasable Rapidspheres™
Scientific Poster
Positive Selection of PE- or Biotin-Conjugated Antibody Labeled Cells with Releasable Rapidspheres�...

文献 (1)

TCF-1 promotes chromatin interactions across topologically associating domains in T cell progenitors. W. Wang et al. Nature immunology 2022 jul

Abstract

The high mobility group (HMG) transcription factor TCF-1 is essential for early T cell development. Although in vitro biochemical assays suggest that HMG proteins can serve as architectural elements in the assembly of higher-order nuclear organization, the contribution of TCF-1 on the control of three-dimensional (3D) genome structures during T cell development remains unknown. Here, we investigated the role of TCF-1 in 3D genome reconfiguration. Using gain- and loss-of-function experiments, we discovered that the co-occupancy of TCF-1 and the architectural protein CTCF altered the structure of topologically associating domains in T cell progenitors, leading to interactions between previously insulated regulatory elements and target genes at late stages of T cell development. The TCF-1-dependent gain in long-range interactions was linked to deposition of active enhancer mark H3K27ac and recruitment of the cohesin-loading factor NIPBL at active enhancers. These data indicate that TCF-1 has a role in controlling global genome organization during T cell development.
View publication
View All Publications

更多信息

更多信息
种属 Mouse
Magnet Compatibility • EasySep™ Magnet (Catalog #18000) • “The Big Easy” EasySep™ Magnet (Catalog #18001) • EasyPlate™ Magnet (Catalog #18102) • EasyEights™ Magnet (Catalog #18103)
样本来源 Bone Marrow, Other, Spleen
Selection Method Positive
标记抗体
  1. 山羊抗小鼠 IgG (H+L) 抗体，多克隆
    山羊抗小鼠 IgG (H+L) 抗体，多克隆

    抗小鼠IgG（H+L）山羊多克隆抗体

质量保证:

产品仅供研究使用，不用于针对人或动物的诊断或治疗。 欲获悉更多关于STEMCELL的质控信息，请访问 STEMCELL.CN/COMPLIANCE.
Copyright © 2025 by STEMCELL Technologies. All rights reserved.