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ImmunoCult™-ACF 人B细胞扩增添加物

无血清、无动物成分的培养添加物，用于人B细胞扩增
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产品号 #10974_C

无血清、无动物成分的培养添加物，用于人B细胞扩增

产品优势

  • 无需血清、饲养层细胞或专用培养板即可实现人B细胞的强力体外扩增
  • 无血清、无动物成分的添加物。

产品组分包括

  • ImmunoCult™-ACF 人B细胞扩增添加物，2 mL
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  1. ImmunoCult™-XF B Cell Base Medium
    ImmunoCult™-XF B Cell Base Medium

    Serum-free and xeno-free base medium for the expansion of B cells

总览
实验数据
产品说明书及文档
应用领域
相关材料与文献
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总览

ImmunoCult™-ACF 人B细胞扩增添加物是一种无血清且无动物成分的添加物，专为在无血清、饲养层细胞或专用培养板的条件下促进人外周血B细胞的强力体外扩增而设计。该添加物含有重组蛋白和化学上成分明确的组分，可支持人B细胞的活化、扩增及其向浆细胞的成熟。

该产品作为ImmunoCult™ 人B细胞扩增试剂盒的组分，也可单独购买。ImmunoCult™-ACF 人B细胞扩增添加物为50倍浓缩添加物，可添加至基础培养基（如ImmunoCult™-XF B细胞基础培养基）中，配制成用于培养和扩增人B细胞的无血清完全培养基。

该产品作为ImmunoCult™ 人B细胞扩增试剂盒的组分，也可单独购买。ImmunoCult™-ACF 人B细胞扩增添加物为50倍浓缩添加物，可添加至基础培养基（如ImmunoCult™-XF B细胞基础培养基）中，配制成用于培养和扩增人B细胞的无血清完全培养基。

该产品作为ImmunoCult™ 人B细胞扩增试剂盒的组分，也可单独购买。ImmunoCult™-ACF 人B细胞扩增添加物为50倍浓缩添加物，可添加至基础培养基（如ImmunoCult™-XF B细胞基础培养基）中，配制成用于培养和扩增人B细胞的无血清完全培养基。

该产品作为ImmunoCult™ 人B细胞扩增试剂盒的组分，也可单独购买。ImmunoCult™-ACF 人B细胞扩增添加物为50倍浓缩添加物，可添加至基础培养基（如ImmunoCult™-XF B细胞基础培养基）中，配制成用于培养和扩增人B细胞的无血清完全培养基。

包含
重组蛋白，including CD40L and other cytokines Chemically defined components
 
亚型
添加剂
 
细胞类型
B 细胞
 
种属
人
 
应用
激活，细胞培养，分化，扩增，基因组编辑
 
品牌
ImmunoCult
 
研究领域
抗体​制备，免疫，感染性疾病(传染病)
 
制剂类别
Animal Component-Free，无血清
 

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实验数据

Fold Expansion and Maturation of B Cells Cultured with ImmunoCult™ Human B Cell Expansion Kit

Figure 1. Expansion and Maturation of Human B Cells with ImmunoCult™ Human B Cell Expansion Kit

B cells isolated from human peripheral blood mononuclear cells (PBMCs) (leukopak) using EasySep™ Human Pan-B Cell Enrichment Kit (Catalog #19554) were seeded at 1 x 10^5 cells/well in 24-well tissue culture plates with ImmunoCult™-ACF Human B Cell Expansion Supplement and ImmunoCult™-XF B Cell Base Medium included in the ImmunoCult™ Human B Cell Expansion Kit. The cells were passaged every 3 - 4 days. (A) Fold expansion of viable cells is shown for n = 12 donors, with bars representing the mean and 95% confidence level (range 38- to 1190-fold at day 14 ± 1 day). (B) Expression of CD138 and CD20 was analyzed by flow cytometry at each timepoint (data represent % positive viable cells; mean ± 1 SD). The observed changes indicate maturation of B cells to plasma cells/blasts.

Microscopy Image of Human B Cells Expanded In Vitro Without the Use of Serum, Feeder Cells, or Specialized Culture Plates

Figure 2. Light Microscopy Image of Cultured Human B Cells

B cells isolated from human PBMCs (leukopak) using EasySep™ Human Pan-B Cell Enrichment Kit were seeded at 1 x 10^5 cells/well in a 24-well tissue culture plate and cultured with the ImmunoCult™ Human B Cell Expansion Kit. The cells were passaged on day 4 after seeding and imaged at 40X magnification on day 6.

Expansion and Viability of Human Memory B Cells Cultured with ImmunoCult™ Human B Cell Expansion Kit

Figure 3. Expansion and Viability of Human Memory B Cells Cultured with ImmunoCult™ Human B Cell Expansion Kit

Memory B cells were isolated from human PBMCs (leukopak) using EasySep™ Human Memory B Cell Isolation Kit and were seeded at 0.5 x 10^5 cells/well in 48-well tissue culture plates and cultured with the ImmunoCult™ Human B Cell Expansion Kit. The cells were passaged every 2 - 4 days and the fold expansion of viable cells (A) and cell viability (B) were calculated at each timepoint. Data represent the mean ± 1 SD of triplicate cultures for the same donor.

Expansion and Viability of Human Naive B Cells Cultured with ImmunoCult™ Human B Cell Expansion Kit

Figure 4. Expansion and Viability of Human Naïve B Cells Cultured with ImmunoCult™ Human B Cell Expansion Kit

Naïve B cells were isolated from human PBMCs (leukopak) using EasySep™ Human Memory B Cell Isolation Kit and were seeded at 0.5 x 10^5 cells/well in 48-well tissue culture plates and cultured with the ImmunoCult™ Human B Cell Expansion Kit. The cells were passaged every 2 - 4 days and the fold expansion of viable cells (A) and cell viability (B) were calculated at each timepoint. Data represent the mean ± 1 SD of triplicate cultures for the same donor.

产品说明书及文档

请在《产品说明书》中查找相关支持信息和使用说明，或浏览下方更多实验方案。

Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet 1
Product Name
ImmunoCult™-ACF Human B Cell Expansion Supplement
Catalog #
10974
Lot #
All
Language
English
Document Type
Product Information Sheet 2
Product Name
ImmunoCult™-ACF Human B Cell Expansion Supplement
Catalog #
10974
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
ImmunoCult™-ACF Human B Cell Expansion Supplement
Catalog #
10974
Lot #
All
Language
English

应用领域

本产品专为以下研究领域设计，适用于工作流程中的高亮阶段。探索这些工作流程，了解更多我们为各研究领域提供的其他配套产品。

Research Area
Workflow Stages

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更多信息
种属 Human
Contains Recombinant proteins, including CD40L and other cytokines Chemically defined components
配方类别 Animal Component-Free, Serum-Free
质量保证:

产品仅供研究使用，不用于针对人或动物的诊断或治疗。 欲获悉更多关于STEMCELL的质控信息，请访问 STEMCELL.CN/COMPLIANCE.
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