产品号 #10974_C
无血清、无动物成分的培养添加物,用于人B细胞扩增
若您需要咨询产品或有任何技术问题,请通过官方电话 400 885 9050 或邮箱 info.cn@stemcell.com 与我们联系
Serum-free and xeno-free base medium for the expansion of B cells
Compatible antibodies for purity assessment of isolated cells
ImmunoCult™-ACF 人B细胞扩增添加物是一种无血清且无动物成分的添加物,专为在无血清、饲养层细胞或专用培养板的条件下促进人外周血B细胞的强力体外扩增而设计。该添加物含有重组蛋白和化学上成分明确的组分,可支持人B细胞的活化、扩增及其向浆细胞的成熟。
该产品作为ImmunoCult™ 人B细胞扩增试剂盒的组分,也可单独购买。ImmunoCult™-ACF 人B细胞扩增添加物为50倍浓缩添加物,可添加至基础培养基(如ImmunoCult™-XF B细胞基础培养基)中,配制成用于培养和扩增人B细胞的无血清完全培养基。
该产品作为ImmunoCult™ 人B细胞扩增试剂盒的组分,也可单独购买。ImmunoCult™-ACF 人B细胞扩增添加物为50倍浓缩添加物,可添加至基础培养基(如ImmunoCult™-XF B细胞基础培养基)中,配制成用于培养和扩增人B细胞的无血清完全培养基。
该产品作为ImmunoCult™ 人B细胞扩增试剂盒的组分,也可单独购买。ImmunoCult™-ACF 人B细胞扩增添加物为50倍浓缩添加物,可添加至基础培养基(如ImmunoCult™-XF B细胞基础培养基)中,配制成用于培养和扩增人B细胞的无血清完全培养基。
该产品作为ImmunoCult™ 人B细胞扩增试剂盒的组分,也可单独购买。ImmunoCult™-ACF 人B细胞扩增添加物为50倍浓缩添加物,可添加至基础培养基(如ImmunoCult™-XF B细胞基础培养基)中,配制成用于培养和扩增人B细胞的无血清完全培养基。
包含
重组蛋白,including CD40L and other cytokines
Chemically defined components
亚型
添加剂
细胞类型
B 细胞
种属
人
应用
激活,细胞培养,分化,扩增,基因组编辑
品牌
ImmunoCult
研究领域
抗体制备,免疫,感染性疾病(传染病)
制剂类别
Animal Component-Free,无血清
Figure 1. Expansion and Maturation of Human B Cells with ImmunoCult™ Human B Cell Expansion Kit
B cells isolated from human peripheral blood mononuclear cells (PBMCs) (leukopak) using EasySep™ Human Pan-B Cell Enrichment Kit (Catalog #19554) were seeded at 1 x 10^5 cells/well in 24-well tissue culture plates with ImmunoCult™-ACF Human B Cell Expansion Supplement and ImmunoCult™-XF B Cell Base Medium included in the ImmunoCult™ Human B Cell Expansion Kit. The cells were passaged every 3 - 4 days. (A) Fold expansion of viable cells is shown for n = 12 donors, with bars representing the mean and 95% confidence level (range 38- to 1190-fold at day 14 ± 1 day). (B) Expression of CD138 and CD20 was analyzed by flow cytometry at each timepoint (data represent % positive viable cells; mean ± 1 SD). The observed changes indicate maturation of B cells to plasma cells/blasts.
Figure 2. Light Microscopy Image of Cultured Human B Cells
B cells isolated from human PBMCs (leukopak) using EasySep™ Human Pan-B Cell Enrichment Kit were seeded at 1 x 10^5 cells/well in a 24-well tissue culture plate and cultured with the ImmunoCult™ Human B Cell Expansion Kit. The cells were passaged on day 4 after seeding and imaged at 40X magnification on day 6.
Figure 3. Expansion and Viability of Human Memory B Cells Cultured with ImmunoCult™ Human B Cell Expansion Kit
Memory B cells were isolated from human PBMCs (leukopak) using EasySep™ Human Memory B Cell Isolation Kit and were seeded at 0.5 x 10^5 cells/well in 48-well tissue culture plates and cultured with the ImmunoCult™ Human B Cell Expansion Kit. The cells were passaged every 2 - 4 days and the fold expansion of viable cells (A) and cell viability (B) were calculated at each timepoint. Data represent the mean ± 1 SD of triplicate cultures for the same donor.
Figure 4. Expansion and Viability of Human Naïve B Cells Cultured with ImmunoCult™ Human B Cell Expansion Kit
Naïve B cells were isolated from human PBMCs (leukopak) using EasySep™ Human Memory B Cell Isolation Kit and were seeded at 0.5 x 10^5 cells/well in 48-well tissue culture plates and cultured with the ImmunoCult™ Human B Cell Expansion Kit. The cells were passaged every 2 - 4 days and the fold expansion of viable cells (A) and cell viability (B) were calculated at each timepoint. Data represent the mean ± 1 SD of triplicate cultures for the same donor.
请在《产品说明书》中查找相关支持信息和使用说明,或浏览下方更多实验方案。
本产品专为以下研究领域设计,适用于工作流程中的高亮阶段。探索这些工作流程,了解更多我们为各研究领域提供的其他配套产品。
Thank you for your interest in IntestiCult™ Organoid Growth Medium (Human). Please provide us with your contact information and your local representative will contact you with a customized quote. Where appropriate, they can also assist you with a(n):
Estimated delivery time for your area
Product sample or exclusive offer
In-lab demonstration
种属 | Human |
---|---|
Contains | Recombinant proteins, including CD40L and other cytokines Chemically defined components |
配方类别 | Animal Component-Free, Serum-Free |
扩增B细胞的无血清培养试剂盒
采用可解离磁珠对人记忆B细胞进行免疫磁珠分选
免疫磁珠负选人B细胞
冻存的人原代细胞
扫描二维码或搜索微信号STEMCELLTech,即可关注我们的微信平台,第一时间接收丰富的技术资源和最新的活动信息。
如您有任何问题,欢迎发消息给STEMCELLTech微信公众平台,或与我们通过电话/邮件联系:400 885 9050 INFO.CN@STEMCELL.COM。