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ImmunoCult™ 人B细胞扩增试剂盒

扩增B细胞的无血清培养试剂盒

产品号 #(选择产品)

产品号 #100-0645_C

扩增B细胞的无血清培养试剂盒

产品优势

  • 无需使用血清、饲养细胞或专用培养板,即可在体外高效扩增人类B细胞
  • 无血清培养基和无动物源成分添加剂

产品组分包括

  • ImmunoCult™-XF B细胞基础培养基,100ml;
  • ImmunoCult™-ACF人B细胞扩增添加剂,2ml

总览

无需使用血清、饲养层细胞或专用培养板,即可实现人B细胞的稳定扩增用于下游检测和应用。

ImmunoCult™人B细胞扩增试剂盒包括ImmunoCult™-ACF人B细胞扩增添加剂和ImmunoCult™-XF B细胞基础培养基,两者共同作用实现人B细胞的持续激活和扩增,并使其成熟为浆细胞。仅需一周或更短时间的培养,即可收获B细胞并直接用于下游应用。为了方便起见,试剂盒中的所有组分也可单独购买。

该试剂盒与我们许多其它上游和下游产品兼容。例如,您可以使用EasySep™细胞分选试剂盒分选各种B细胞亚群-如Pan-B细胞,记忆B细胞和初始B细胞,分选后可立即使用ImmunoCult™人B细胞扩增试剂盒进行扩增培养。

ImmunoCult™人B细胞扩增试剂盒包括ImmunoCult™-ACF人B细胞扩增添加剂和ImmunoCult™-XF B细胞基础培养基,两者共同作用实现人B细胞的持续激活和扩增,并使其成熟为浆细胞。仅需一周或更短时间的培养,即可收获B细胞并直接用于下游应用。为了方便起见,试剂盒中的所有组分也可单独购买。

该试剂盒与我们许多其它上游和下游产品兼容。例如,您可以使用EasySep™细胞分选试剂盒分选各种B细胞亚群-如Pan-B细胞,记忆B细胞和初始B细胞,分选后可立即使用ImmunoCult™人B细胞扩增试剂盒进行扩增培养。

ImmunoCult™人B细胞扩增试剂盒包括ImmunoCult™-ACF人B细胞扩增添加剂和ImmunoCult™-XF B细胞基础培养基,两者共同作用实现人B细胞的持续激活和扩增,并使其成熟为浆细胞。仅需一周或更短时间的培养,即可收获B细胞并直接用于下游应用。为了方便起见,试剂盒中的所有组分也可单独购买。

该试剂盒与我们许多其它上游和下游产品兼容。例如,您可以使用EasySep™细胞分选试剂盒分选各种B细胞亚群-如Pan-B细胞,记忆B细胞和初始B细胞,分选后可立即使用ImmunoCult™人B细胞扩增试剂盒进行扩增培养。

ImmunoCult™人B细胞扩增试剂盒包括ImmunoCult™-ACF人B细胞扩增添加剂和ImmunoCult™-XF B细胞基础培养基,两者共同作用实现人B细胞的持续激活和扩增,并使其成熟为浆细胞。仅需一周或更短时间的培养,即可收获B细胞并直接用于下游应用。为了方便起见,试剂盒中的所有组分也可单独购买。

该试剂盒与我们许多其它上游和下游产品兼容。例如,您可以使用EasySep™细胞分选试剂盒分选各种B细胞亚群-如Pan-B细胞,记忆B细胞和初始B细胞,分选后可立即使用ImmunoCult™人B细胞扩增试剂盒进行扩增培养。

亚型
专用培养基,添加剂
 
细胞类型
B 细胞
 
种属

 
应用
细胞培养
 
品牌
ImmunoCult
 
研究领域
抗体​制备,药物发现和毒理检测,免疫,感染性疾病(传染病),干细胞生物学,细胞治疗开发
 
制剂类别
无血清,Xeno-Free
 

实验数据

Fold Expansion and Maturation of B Cells Cultured with ImmunoCult™ Human B Cell Expansion Kit

Figure 1. Expansion and Maturation of Human B Cells with ImmunoCult™ Human B Cell Expansion Kit

B cells isolated from human peripheral blood mononuclear cells (PBMCs) (leukopak) using EasySep™ Human Pan-B Cell Enrichment Kit (Catalog #19554) were seeded at 1 x 10^5 cells/well in 24-well tissue culture plates with ImmunoCult™-ACF Human B Cell Expansion Supplement and ImmunoCult™-XF B Cell Base Medium included in the ImmunoCult™ Human B Cell Expansion Kit. The cells were passaged every 3 - 4 days. (A) Fold expansion of viable cells is shown for n = 12 donors, with bars representing the mean and 95% confidence level (range 38- to 1190-fold at day 14 ± 1 day). (B) Expression of CD138 and CD20 was analyzed by flow cytometry at each timepoint (data represent % positive viable cells; mean ± 1 SD). The observed changes indicate maturation of B cells to plasma cells/blasts.

Microscopy Image of Human B Cells Expanded In Vitro Without the Use of Serum, Feeder Cells, or Specialized Culture Plates

Figure 2. Light Microscopy Image of Cultured Human B Cells

B cells isolated from human PBMCs (leukopak) using EasySep™ Human Pan-B Cell Enrichment Kit were seeded at 1 x 10^5 cells/well in a 24-well tissue culture plate and cultured with the ImmunoCult™ Human B Cell Expansion Kit. The cells were passaged on day 4 after seeding and imaged at 40X magnification on day 6.

Expansion and Viability of Human Memory B Cells Cultured with ImmunoCult™ Human B Cell Expansion Kit

Figure 3. Expansion and Viability of Human Memory B Cells Cultured with ImmunoCult™ Human B Cell Expansion Kit

Memory B cells were isolated from human PBMCs (leukopak) using EasySep™ Human Memory B Cell Isolation Kit and were seeded at 0.5 x 10^5 cells/well in 48-well tissue culture plates and cultured with the ImmunoCult™ Human B Cell Expansion Kit. The cells were passaged every 2 - 4 days and the fold expansion of viable cells (A) and cell viability (B) were calculated at each timepoint. Data represent the mean ± 1 SD of triplicate cultures for the same donor.

Expansion and Viability of Human Naive B Cells Cultured with ImmunoCult™ Human B Cell Expansion Kit

Figure 4. Expansion and Viability of Human Naïve B Cells Cultured with ImmunoCult™ Human B Cell Expansion Kit

Naïve B cells were isolated from human PBMCs (leukopak) using EasySep™ Human Memory B Cell Isolation Kit and were seeded at 0.5 x 10^5 cells/well in 48-well tissue culture plates and cultured with the ImmunoCult™ Human B Cell Expansion Kit. The cells were passaged every 2 - 4 days and the fold expansion of viable cells (A) and cell viability (B) were calculated at each timepoint. Data represent the mean ± 1 SD of triplicate cultures for the same donor.

产品说明书及文档

请在《产品说明书》中查找相关支持信息和使用说明,或浏览下方更多实验方案。

Document Type
Product Name
Catalog #
Lot #
Language
Catalog #
100-0645
Lot #
All
Language
English
Catalog #
100-0645
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Catalog #
100-0645
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Catalog #
100-0645
Lot #
All
Language
English

应用领域

本产品专为以下研究领域设计,适用于工作流程中的高亮阶段。探索这些工作流程,了解更多我们为各研究领域提供的其他配套产品。

更多信息

更多信息
种属 Human
配方类别 Serum-Free, Xeno-Free
质量保证:

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