ImmunoCult™ Human B Cell Expansion Kit

总览

无需使用血清、饲养层细胞或专用培养板，即可实现人B细胞的稳定扩增用于下游检测和应用。

ImmunoCult™人B细胞扩增试剂盒包括ImmunoCult™-ACF人B细胞扩增添加剂和ImmunoCult™-XF B细胞基础培养基，两者共同作用实现人B细胞的持续激活和扩增，并使其成熟为浆细胞。仅需一周或更短时间的培养，即可收获B细胞并直接用于下游应用。为了方便起见，试剂盒中的所有组分也可单独购买。

该试剂盒与我们许多其它上游和下游产品兼容。例如，您可以使用EasySep™细胞分选试剂盒分选各种B细胞亚群-如Pan-B细胞，记忆B细胞和初始B细胞，分选后可立即使用ImmunoCult™人B细胞扩增试剂盒进行扩增培养。

ImmunoCult™人B细胞扩增试剂盒包括ImmunoCult™-ACF人B细胞扩增添加剂和ImmunoCult™-XF B细胞基础培养基，两者共同作用实现人B细胞的持续激活和扩增，并使其成熟为浆细胞。仅需一周或更短时间的培养，即可收获B细胞并直接用于下游应用。为了方便起见，试剂盒中的所有组分也可单独购买。

该试剂盒与我们许多其它上游和下游产品兼容。例如，您可以使用EasySep™细胞分选试剂盒分选各种B细胞亚群-如Pan-B细胞，记忆B细胞和初始B细胞，分选后可立即使用ImmunoCult™人B细胞扩增试剂盒进行扩增培养。

ImmunoCult™人B细胞扩增试剂盒包括ImmunoCult™-ACF人B细胞扩增添加剂和ImmunoCult™-XF B细胞基础培养基，两者共同作用实现人B细胞的持续激活和扩增，并使其成熟为浆细胞。仅需一周或更短时间的培养，即可收获B细胞并直接用于下游应用。为了方便起见，试剂盒中的所有组分也可单独购买。

该试剂盒与我们许多其它上游和下游产品兼容。例如，您可以使用EasySep™细胞分选试剂盒分选各种B细胞亚群-如Pan-B细胞，记忆B细胞和初始B细胞，分选后可立即使用ImmunoCult™人B细胞扩增试剂盒进行扩增培养。

ImmunoCult™人B细胞扩增试剂盒包括ImmunoCult™-ACF人B细胞扩增添加剂和ImmunoCult™-XF B细胞基础培养基，两者共同作用实现人B细胞的持续激活和扩增，并使其成熟为浆细胞。仅需一周或更短时间的培养，即可收获B细胞并直接用于下游应用。为了方便起见，试剂盒中的所有组分也可单独购买。

该试剂盒与我们许多其它上游和下游产品兼容。例如，您可以使用EasySep™细胞分选试剂盒分选各种B细胞亚群-如Pan-B细胞，记忆B细胞和初始B细胞，分选后可立即使用ImmunoCult™人B细胞扩增试剂盒进行扩增培养。

亚型
专用培养基，添加剂

细胞类型
B 细胞

种属
人

应用
细胞培养

品牌
ImmunoCult

研究领域
抗体制备，药物发现和毒理检测，免疫，感染性疾病(传染病)，干细胞生物学，细胞治疗开发

制剂类别
无血清，Xeno-Free

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实验数据

Fold Expansion and Maturation of B Cells Cultured with ImmunoCult™ Human B Cell Expansion Kit

Figure 1. Expansion and Maturation of Human B Cells with ImmunoCult™ Human B Cell Expansion Kit

B cells isolated from human peripheral blood mononuclear cells (PBMCs) (leukopak) using EasySep™ Human Pan-B Cell Enrichment Kit (Catalog #19554) were seeded at 1 x 10^5 cells/well in 24-well tissue culture plates with ImmunoCult™-ACF Human B Cell Expansion Supplement and ImmunoCult™-XF B Cell Base Medium included in the ImmunoCult™ Human B Cell Expansion Kit. The cells were passaged every 3 - 4 days. (A) Fold expansion of viable cells is shown for n = 12 donors, with bars representing the mean and 95% confidence level (range 38- to 1190-fold at day 14 ± 1 day). (B) Expression of CD138 and CD20 was analyzed by flow cytometry at each timepoint (data represent % positive viable cells; mean ± 1 SD). The observed changes indicate maturation of B cells to plasma cells/blasts.

Microscopy Image of Human B Cells Expanded In Vitro Without the Use of Serum, Feeder Cells, or Specialized Culture Plates

Figure 2. Light Microscopy Image of Cultured Human B Cells

B cells isolated from human PBMCs (leukopak) using EasySep™ Human Pan-B Cell Enrichment Kit were seeded at 1 x 10^5 cells/well in a 24-well tissue culture plate and cultured with the ImmunoCult™ Human B Cell Expansion Kit. The cells were passaged on day 4 after seeding and imaged at 40X magnification on day 6.

Figure 3. Expansion and Viability of Human Memory B Cells Cultured with ImmunoCult™ Human B Cell Expansion Kit

Memory B cells were isolated from human PBMCs (leukopak) using EasySep™ Human Memory B Cell Isolation Kit and were seeded at 0.5 x 10^5 cells/well in 48-well tissue culture plates and cultured with the ImmunoCult™ Human B Cell Expansion Kit. The cells were passaged every 2 - 4 days and the fold expansion of viable cells (A) and cell viability (B) were calculated at each timepoint. Data represent the mean ± 1 SD of triplicate cultures for the same donor.

Expansion and Viability of Human Naive B Cells Cultured with ImmunoCult™ Human B Cell Expansion Kit

Figure 4. Expansion and Viability of Human Naïve B Cells Cultured with ImmunoCult™ Human B Cell Expansion Kit

Naïve B cells were isolated from human PBMCs (leukopak) using EasySep™ Human Memory B Cell Isolation Kit and were seeded at 0.5 x 10^5 cells/well in 48-well tissue culture plates and cultured with the ImmunoCult™ Human B Cell Expansion Kit. The cells were passaged every 2 - 4 days and the fold expansion of viable cells (A) and cell viability (B) were calculated at each timepoint. Data represent the mean ± 1 SD of triplicate cultures for the same donor.