产品号 #10953_C
小鼠naïve CD4+ T细胞向Th1细胞分化的无血清培养补充
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RPMI 1640 medium
Compatible antibodies for purity assessment of isolated cells
ImmunoCult™小鼠Th1分化补充剂含有重组小鼠细胞因子和阻断单克隆抗体的组合,用于促进小鼠naïve CD4+ T细胞向Th1细胞的分化。该补充剂与含有胎牛血清和其他添加剂的RPMI 1640培养基(产品号#36750)以及抗小鼠CD3和抗小鼠CD28单克隆抗体作为活化剂体系搭配使用。
本产品推荐用于:
•研究小鼠naïve T细胞分化和Th1功能的调节
•开发将naïve T细胞扩增以及向Th1亚群极化的工艺流程
本产品推荐用于:
•研究小鼠naïve T细胞分化和Th1功能的调节
•开发将naïve T细胞扩增以及向Th1亚群极化的工艺流程
本产品推荐用于:
•研究小鼠naïve T细胞分化和Th1功能的调节
•开发将naïve T细胞扩增以及向Th1亚群极化的工艺流程
本产品推荐用于:
•研究小鼠naïve T细胞分化和Th1功能的调节
•开发将naïve T细胞扩增以及向Th1亚群极化的工艺流程
包含
• Recombinant mouse interleukin 2 (IL-2)
• Recombinant mouse interleukin 12 (IL-12)
• Rat anti-mouse interleukin 4 (anti-IL-4)
亚型
添加剂
细胞类型
T 细胞,T 细胞,CD4+
种属
小鼠
应用
细胞培养,分化,扩增
品牌
ImmunoCult
研究领域
免疫
Figure 1. ImmunoCult™ Mouse Th1 Differentiation Supplement Produces CD4+IFNγ+T-Bet+ Cells Under Th1 Polarizing Conditions
Naïve CD4+ T cells were isolated from mouse splenocytes using the EasySep™ Mouse Naive CD4+ T Cell Isolation Kit (Catalog #19765), activated with plate-bound anti-CD3 and soluble anti-CD28 and cultured in medium alone (Non-polarized cultures), or medium supplemented with ImmunoCult™ Mouse Th1 Differentiation Supplement (Polarized cultures ) for 5 days. Cells were subsequently stimulated with PMA/Ionomycin, in conjunction with brefeldin A treatment, followed by staining with anti-CD4, anti-IFNγ, anti-T-bet and a viability dye and analyzed by flow cytometry. Shown is the expression of IFNγ and T-bet, back-gated on viable, CD4+ cells from non-polarized (A) or polarized cultures (B). The mean proportion of CD4+IFNγ+T-Bet+ cells is significantly higher in cells cultured in ImmunoCult™ Mouse Th1 Differentiation Supplement (44 ± 6%) compared to non-polarized cells (2 ± 1%) (p < 0.001; n = 13). Data from experimental groups were compared using a paired T-test.
Figure 2. ImmunoCult™ Mouse Th1 Differentiation Supplement Expands Total Nucleated Cells Under Th1 Polarizing Conditions
Naïve CD4+ T cells were isolated from mouse splenocytes using the EasySep™ Mouse Naive CD4+ T Cell Isolation Kit (Catalog #19765), activated with plate-bound anti-CD3 and soluble anti-CD28 and cultured in medium alone, or medium containing ImmunoCult™ Mouse Th1 Differentiation Supplement for 5 days. A 4.5-fold expansion of total nuclear cells (TNC) ensues in cultures polarized with ImmunoCult Mouse Th1 Differentiation Supplement (purple column), which is significantly higher compared to non-polarized cultures (grey column). (Data represent the mean ± SEM, n = 11 - 20, *p < 0.05. Data from experimental groups were compared using a paired T-test).
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本产品专为以下研究领域设计,适用于工作流程中的高亮阶段。探索这些工作流程,了解更多我们为各研究领域提供的其他配套产品。
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种属 | Mouse |
---|---|
Contains | • Recombinant mouse interleukin 2 (IL-2) • Recombinant mouse interleukin 12 (IL-12) • Rat anti-mouse interleukin 4 (anti-IL-4) |
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