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ImmunoCult™ 小鼠Th1分化添加剂

小鼠naïve CD4+ T细胞向Th1细胞分化的无血清培养补充
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产品号 #（选择产品）

产品号 #10953_C

小鼠naïve CD4+ T细胞向Th1细胞分化的无血清培养补充

产品优势

  • 为诱导从C57BL/6小鼠脾脏分选的初始CD4+T细胞向Th1分化而优化；
  • 作为100X浓缩物供应；解冻和混匀后，管内容物可直接添加到培养基中
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  1. RPMI 1640 Medium
    RPMI 1640 Medium

    RPMI 1640 medium

总览
实验数据
产品说明书及文档
应用领域
相关材料与文献
相关产品

总览

ImmunoCult™小鼠Th1分化补充剂含有重组小鼠细胞因子和阻断单克隆抗体的组合，用于促进小鼠naïve CD4+ T细胞向Th1细胞的分化。该补充剂与含有胎牛血清和其他添加剂的RPMI 1640培养基（产品号#36750）以及抗小鼠CD3和抗小鼠CD28单克隆抗体作为活化剂体系搭配使用。

本产品推荐用于：

•研究小鼠naïve T细胞分化和Th1功能的调节

•开发将naïve T细胞扩增以及向Th1亚群极化的工艺流程

本产品推荐用于：

•研究小鼠naïve T细胞分化和Th1功能的调节

•开发将naïve T细胞扩增以及向Th1亚群极化的工艺流程

本产品推荐用于：

•研究小鼠naïve T细胞分化和Th1功能的调节

•开发将naïve T细胞扩增以及向Th1亚群极化的工艺流程

本产品推荐用于：

•研究小鼠naïve T细胞分化和Th1功能的调节

•开发将naïve T细胞扩增以及向Th1亚群极化的工艺流程

包含
• Recombinant mouse interleukin 2 (IL-2) • Recombinant mouse interleukin 12 (IL-12) • Rat anti-mouse interleukin 4 (anti-IL-4)
 
亚型
添加剂
 
细胞类型
T 细胞，T 细胞，CD4+
 
种属
小鼠
 
应用
细胞培养，分化，扩增
 
品牌
ImmunoCult
 
研究领域
免疫
 

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实验数据

Figure 1. ImmunoCult™ Mouse Th1 Differentiation Supplement Produces CD4+IFNγ+T-Bet+ Cells Under Th1 Polarizing Conditions

Naïve CD4+ T cells were isolated from mouse splenocytes using the EasySep™ Mouse Naive CD4+ T Cell Isolation Kit (Catalog #19765), activated with plate-bound anti-CD3 and soluble anti-CD28 and cultured in medium alone (Non-polarized cultures), or medium supplemented with ImmunoCult™ Mouse Th1 Differentiation Supplement (Polarized cultures ) for 5 days. Cells were subsequently stimulated with PMA/Ionomycin, in conjunction with brefeldin A treatment, followed by staining with anti-CD4, anti-IFNγ, anti-T-bet and a viability dye and analyzed by flow cytometry. Shown is the expression of IFNγ and T-bet, back-gated on viable, CD4+ cells from non-polarized (A) or polarized cultures (B). The mean proportion of CD4+IFNγ+T-Bet+ cells is significantly higher in cells cultured in ImmunoCult™ Mouse Th1 Differentiation Supplement (44 ± 6%) compared to non-polarized cells (2 ± 1%) (p < 0.001; n = 13). Data from experimental groups were compared using a paired T-test.

Figure 2. ImmunoCult™ Mouse Th1 Differentiation Supplement Expands Total Nucleated Cells Under Th1 Polarizing Conditions

Naïve CD4+ T cells were isolated from mouse splenocytes using the EasySep™ Mouse Naive CD4+ T Cell Isolation Kit (Catalog #19765), activated with plate-bound anti-CD3 and soluble anti-CD28 and cultured in medium alone, or medium containing ImmunoCult™ Mouse Th1 Differentiation Supplement for 5 days. A 4.5-fold expansion of total nuclear cells (TNC) ensues in cultures polarized with ImmunoCult Mouse Th1 Differentiation Supplement (purple column), which is significantly higher compared to non-polarized cultures (grey column). (Data represent the mean ± SEM, n = 11 - 20, *p < 0.05. Data from experimental groups were compared using a paired T-test).

产品说明书及文档

请在《产品说明书》中查找相关支持信息和使用说明，或浏览下方更多实验方案。

Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet
Product Name
ImmunoCult™ Mouse Th1 Differentiation Supplement
Catalog #
10953
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
ImmunoCult™ Mouse Th1 Differentiation Supplement
Catalog #
10953
Lot #
All
Language
English

应用领域

本产品专为以下研究领域设计，适用于工作流程中的高亮阶段。探索这些工作流程，了解更多我们为各研究领域提供的其他配套产品。

Research Area
Workflow Stages

更多信息

更多信息
种属 Mouse
Contains • Recombinant mouse interleukin 2 (IL-2) • Recombinant mouse interleukin 12 (IL-12) • Rat anti-mouse interleukin 4 (anti-IL-4)
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产品仅供研究使用，不用于针对人或动物的诊断或治疗。 欲获悉更多关于STEMCELL的质控信息，请访问 STEMCELL.CN/COMPLIANCE.
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