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mFreSR™

用于人胚胎干细胞和诱导多能干细胞的无血清冻存液

产品号 #(选择产品)

产品号 #05854_C

用于人胚胎干细胞和诱导多能干细胞的无血清冻存液

产品优势

  • 易使用
  • 无血清,专为与TeSR™维持培养基配合使用而优化
  • 细胞复苏效率高
  • 预筛选成分,确保批次间一致性
You may notice that your reagent packaging looks slightly different from images displayed here or from previous orders. Due to pandemic-related plasticware shortages, we are temporarily using alternative bottles for this product. Rest assured that the products themselves and how you should use them have not changed.

总览

mFreSR™是一种无血清的冻存液,用于人胚胎和诱导多能干细胞(ESC和iPSC)的冻存。mFreSR™含有DMSO,成分完整且随时可用。与mTeSR™ 1或mTeSR™ Plus配合使用时,mFreSR™无需使用饲养层和血清。使用mFreSR™冻存的人胚胎干细胞和诱导多能干细胞的复苏效率高于使用血清的传统复苏方法。

细胞类型
多能干细胞
 
种属

 
应用
冻存
 
品牌
mFreSR,TeSR
 
研究领域
干细胞生物学
 
制剂类别
无血清
 

实验数据

mFreSR™ Improves thawing efficiencies 5- to 10-fold over other reported methods

Figure 1. mFreSR™ Improves Thawing Efficiencies 5- to 10-Fold over Other Reported Methods

H9 hESCs were cryopreserved in mFreSR™ at the indicated passage number. Thawing efficiencies were analyzed by counting the number of surviving clumps after thawing.

产品说明书及文档

请在《产品说明书》中查找相关支持信息和使用说明,或浏览下方更多实验方案。

Document Type
Product Name
Catalog #
Lot #
Language
Product Name
mFreSR™
Catalog #
05855, 05854
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
mFreSR™
Catalog #
05855, 05854
Lot #
All
Language
English

应用领域

本产品专为以下研究领域设计,适用于工作流程中的高亮阶段。探索这些工作流程,了解更多我们为各研究领域提供的其他配套产品。

相关材料与文献

技术资料 (14)

文献 (14)

Functional Maturation of Human Stem Cell-Derived Neurons in Long-Term Cultures. Lam RS et al. PloS one 2017

Abstract

Differentiated neurons can be rapidly acquired, within days, by inducing stem cells to express neurogenic transcription factors. We developed a protocol to maintain long-term cultures of human neurons, called iNGNs, which are obtained by inducing Neurogenin-1 and Neurogenin-2 expression in induced pluripotent stem cells. We followed the functional development of iNGNs over months and they showed many hallmark properties for neuronal maturation, including robust electrical and synaptic activity. Using iNGNs expressing a variant of channelrhodopsin-2, called CatCh, we could control iNGN activity with blue light stimulation. In combination with optogenetic tools, iNGNs offer opportunities for studies that require precise spatial and temporal resolution. iNGNs developed spontaneous network activity, and these networks had excitatory glutamatergic synapses, which we characterized with single-cell synaptic recordings. AMPA glutamatergic receptor activity was especially dominant in postsynaptic recordings, whereas NMDA glutamatergic receptor activity was absent from postsynaptic recordings but present in extrasynaptic recordings. Our results on long-term cultures of iNGNs could help in future studies elucidating mechanisms of human synaptogenesis and neurotransmission, along with the ability to scale-up the size of the cultures.
Generation of spinocerebellar ataxia type 3 patient-derived induced pluripotent stem cell line SCA3.B11. Hansen SK et al. Stem Cell Research 2016 MAR

Abstract

Spinocerebellar ataxia type 3 (SCA3) is a dominantly inherited neurodegenerative disease caused by an expansion of the CAG-repeat in ATXN3. In this study, induced pluripotent stem cells (iPSCs) were generated from SCA3 patient dermal fibroblasts by electroporation with episomal plasmids encoding L-MYC, LIN28, SOX2, KLF4, OCT4 and short hairpin RNA targeting P53. The resulting iPSCs had normal karyotype, were free of integrated episomal plasmids, expressed pluripotency markers, could differentiate into the three germ layers in vitro and retained the disease-causing ATXN3 mutation. Potentially, this iPSC line could be a useful tool for the investigation of SCA3 disease mechanisms.
Manifestation of Huntington's disease pathology in human induced pluripotent stem cell-derived neurons. Nekrasov ED et al. Molecular Neurodegeneration 2016 DEC

Abstract

Background: Huntington's disease (HD) is an incurable hereditary neurodegenerative disorder, which manifests itself as a loss of GABAergic medium spiny (GABA MS) neurons in the striatum and caused by an expansion of the CAG repeat in exon 1 of the huntingtin gene. There is no cure for HD, existing pharmaceutical can only relieve its symptoms. Results: Here, induced pluripotent stem cells were established from patients with low CAG repeat expansion in the huntingtin gene, and were then efficiently differentiated into GABA MS-like neurons (GMSLNs) under defined culture conditions. The generated HD GMSLNs recapitulated disease pathology in vitro, as evidenced by mutant huntingtin protein aggregation, increased number of lysosomes/autophagosomes, nuclear indentations, and enhanced neuronal death during cell aging. Moreover, store-operated channel (SOC) currents were detected in the differentiated neurons, and enhanced calcium entry was reproducibly demonstrated in all HD GMSLNs genotypes. Additionally, the quinazoline derivative, EVP4593, reduced the number of lysosomes/autophagosomes and SOC currents in HD GMSLNs and exerted neuroprotective effects during cell aging. Conclusions: Our data is the first to demonstrate the direct link of nuclear morphology and SOC calcium deregulation to mutant huntingtin protein expression in iPSCs-derived neurons with disease-mimetic hallmarks, providing a valuable tool for identification of candidate anti-HD drugs. Our experiments demonstrated that EVP4593 may be a promising anti-HD drug. [ABSTRACT FROM AUTHOR]

更多信息

更多信息
种属 Human
配方类别 Serum-Free
质量保证:

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